Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01875 and MMJJ_RS01880 are separated by 22 nucleotides MMJJ_RS01880 and MMJJ_RS01885 are separated by 52 nucleotides MMJJ_RS01885 and MMJJ_RS01890 are separated by 80 nucleotides
MMJJ_RS01875: MMJJ_RS01875 - global nitrogen regulator NrpR, at 360,058 to 361,668
_RS01875
MMJJ_RS01880: MMJJ_RS01880 - RlmE family RNA methyltransferase, at 361,691 to 362,467
_RS01880
MMJJ_RS01885: MMJJ_RS01885 - KH domain-containing protein, at 362,520 to 363,074
_RS01885
MMJJ_RS01890: MMJJ_RS01890 - serine protein kinase RIO, at 363,155 to 363,976
_RS01890
Position (kb) 361
362
363 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 360.900 kb on - strand, within MMJJ_RS01875 at 360.911 kb on - strand, within MMJJ_RS01875 at 360.912 kb on + strand, within MMJJ_RS01875 at 360.912 kb on + strand, within MMJJ_RS01875 at 361.046 kb on - strand, within MMJJ_RS01875 at 361.085 kb on - strand at 361.112 kb on + strand, within MMJJ_RS01875 at 361.245 kb on + strand at 361.449 kb on + strand, within MMJJ_RS01875 at 361.679 kb on + strand at 361.740 kb on + strand at 361.743 kb on + strand at 361.785 kb on + strand, within MMJJ_RS01880 at 361.848 kb on - strand, within MMJJ_RS01880 at 362.107 kb on - strand, within MMJJ_RS01880 at 362.278 kb on + strand, within MMJJ_RS01880 at 362.280 kb on + strand, within MMJJ_RS01880 at 362.340 kb on - strand, within MMJJ_RS01880 at 362.341 kb on + strand, within MMJJ_RS01880 at 362.478 kb on + strand at 362.481 kb on + strand at 362.482 kb on - strand at 362.491 kb on - strand at 362.491 kb on - strand at 362.492 kb on + strand at 362.505 kb on + strand at 362.516 kb on + strand at 362.516 kb on + strand at 362.522 kb on + strand at 362.535 kb on + strand at 362.550 kb on + strand at 362.716 kb on - strand, within MMJJ_RS01885 at 363.112 kb on + strand at 363.114 kb on - strand at 363.118 kb on - strand at 363.118 kb on - strand at 363.118 kb on - strand at 363.118 kb on - strand at 363.118 kb on - strand at 363.119 kb on + strand at 363.125 kb on - strand at 363.154 kb on + strand at 363.157 kb on + strand at 363.160 kb on + strand at 363.284 kb on + strand at 363.343 kb on + strand, within MMJJ_RS01890 at 363.370 kb on - strand, within MMJJ_RS01890 at 363.398 kb on - strand, within MMJJ_RS01890 at 363.398 kb on - strand, within MMJJ_RS01890
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 360,900 - MMJJ_RS01875 0.52 -2.6 360,911 - MMJJ_RS01875 0.53 -2.3 360,912 + MMJJ_RS01875 0.53 -2.5 360,912 + MMJJ_RS01875 0.53 -2.3 361,046 - MMJJ_RS01875 0.61 -2.8 361,085 - -1.3 361,112 + MMJJ_RS01875 0.65 -2.3 361,245 + -1.5 361,449 + MMJJ_RS01875 0.86 -3.0 361,679 + -1.1 361,740 + -3.7 361,743 + -3.1 361,785 + MMJJ_RS01880 0.12 +0.9 361,848 - MMJJ_RS01880 0.20 -2.8 362,107 - MMJJ_RS01880 0.54 -1.5 362,278 + MMJJ_RS01880 0.76 -2.6 362,280 + MMJJ_RS01880 0.76 -1.5 362,340 - MMJJ_RS01880 0.84 -3.2 362,341 + MMJJ_RS01880 0.84 -1.5 362,478 + -0.6 362,481 + +0.1 362,482 - +0.9 362,491 - +1.0 362,491 - +1.4 362,492 + +0.6 362,505 + -1.3 362,516 + -2.7 362,516 + +1.1 362,522 + -0.8 362,535 + -0.7 362,550 + +0.2 362,716 - MMJJ_RS01885 0.35 -0.5 363,112 + -1.5 363,114 - -0.5 363,118 - +0.5 363,118 - -0.5 363,118 - -0.5 363,118 - -0.2 363,118 - -0.8 363,119 + -1.1 363,125 - -0.2 363,154 + -0.2 363,157 + -0.3 363,160 + +0.1 363,284 + -1.5 363,343 + MMJJ_RS01890 0.23 -1.3 363,370 - MMJJ_RS01890 0.26 -1.3 363,398 - MMJJ_RS01890 0.30 -1.3 363,398 - MMJJ_RS01890 0.30 -2.1
Or see this region's nucleotide sequence