Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01810 and MMJJ_RS01815 are separated by 22 nucleotides MMJJ_RS01815 and MMJJ_RS01820 are separated by 10 nucleotides MMJJ_RS01820 and MMJJ_RS01825 are separated by 105 nucleotides
MMJJ_RS01810: MMJJ_RS01810 - methyl coenzyme M reductase system, component A2, at 347,308 to 348,903
_RS01810
MMJJ_RS01815: MMJJ_RS01815 - DUF134 domain-containing protein, at 348,926 to 349,321
_RS01815
MMJJ_RS01820: MMJJ_RS01820 - MJ1244 family protein, at 349,332 to 349,661
_RS01820
MMJJ_RS01825: MMJJ_RS01825 - DNA repair and recombination protein RadB, at 349,767 to 350,417
_RS01825
Position (kb) 348
349
350 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 348.011 kb on + strand, within MMJJ_RS01810 at 348.036 kb on + strand, within MMJJ_RS01810 at 348.850 kb on - strand at 349.022 kb on - strand, within MMJJ_RS01815 at 349.027 kb on - strand, within MMJJ_RS01815 at 349.046 kb on + strand, within MMJJ_RS01815 at 349.065 kb on + strand, within MMJJ_RS01815 at 349.211 kb on - strand, within MMJJ_RS01815 at 349.252 kb on - strand, within MMJJ_RS01815 at 349.256 kb on - strand, within MMJJ_RS01815 at 349.333 kb on - strand at 349.333 kb on - strand at 349.382 kb on - strand, within MMJJ_RS01820 at 349.382 kb on - strand, within MMJJ_RS01820 at 349.382 kb on - strand, within MMJJ_RS01820 at 349.382 kb on - strand, within MMJJ_RS01820 at 349.383 kb on + strand, within MMJJ_RS01820 at 349.383 kb on + strand, within MMJJ_RS01820 at 349.383 kb on + strand, within MMJJ_RS01820 at 349.383 kb on + strand, within MMJJ_RS01820 at 349.480 kb on + strand, within MMJJ_RS01820 at 349.677 kb on - strand at 349.677 kb on - strand at 349.677 kb on - strand at 349.696 kb on - strand at 349.696 kb on - strand at 349.696 kb on - strand at 349.712 kb on - strand at 349.714 kb on - strand at 349.714 kb on - strand at 349.714 kb on - strand at 349.714 kb on - strand at 349.715 kb on + strand at 349.715 kb on + strand at 349.715 kb on + strand at 349.715 kb on + strand at 349.715 kb on + strand at 349.716 kb on - strand at 349.718 kb on - strand at 349.719 kb on + strand at 349.751 kb on + strand at 349.781 kb on - strand at 349.809 kb on - strand at 349.863 kb on + strand, within MMJJ_RS01825 at 349.863 kb on + strand, within MMJJ_RS01825 at 349.921 kb on + strand, within MMJJ_RS01825 at 349.954 kb on + strand, within MMJJ_RS01825 at 349.954 kb on + strand, within MMJJ_RS01825 at 349.976 kb on - strand, within MMJJ_RS01825 at 350.055 kb on + strand, within MMJJ_RS01825 at 350.093 kb on + strand, within MMJJ_RS01825 at 350.120 kb on - strand, within MMJJ_RS01825 at 350.120 kb on - strand, within MMJJ_RS01825 at 350.121 kb on + strand, within MMJJ_RS01825 at 350.121 kb on + strand, within MMJJ_RS01825 at 350.211 kb on - strand, within MMJJ_RS01825
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 348,011 + MMJJ_RS01810 0.44 -2.2 348,036 + MMJJ_RS01810 0.46 -3.3 348,850 - -0.5 349,022 - MMJJ_RS01815 0.24 -2.2 349,027 - MMJJ_RS01815 0.26 -2.7 349,046 + MMJJ_RS01815 0.30 -1.9 349,065 + MMJJ_RS01815 0.35 -2.1 349,211 - MMJJ_RS01815 0.72 -2.3 349,252 - MMJJ_RS01815 0.82 -2.4 349,256 - MMJJ_RS01815 0.83 -4.1 349,333 - -2.7 349,333 - -2.3 349,382 - MMJJ_RS01820 0.15 -2.2 349,382 - MMJJ_RS01820 0.15 -0.5 349,382 - MMJJ_RS01820 0.15 -1.3 349,382 - MMJJ_RS01820 0.15 -1.2 349,383 + MMJJ_RS01820 0.15 -2.3 349,383 + MMJJ_RS01820 0.15 -3.0 349,383 + MMJJ_RS01820 0.15 -2.5 349,383 + MMJJ_RS01820 0.15 -3.0 349,480 + MMJJ_RS01820 0.45 -1.9 349,677 - +0.1 349,677 - -1.4 349,677 - -0.3 349,696 - -1.3 349,696 - -0.3 349,696 - -3.3 349,712 - -3.4 349,714 - -2.7 349,714 - -2.5 349,714 - -0.9 349,714 - -2.5 349,715 + -2.6 349,715 + -3.6 349,715 + -4.4 349,715 + -3.5 349,715 + -2.7 349,716 - -1.1 349,718 - +0.3 349,719 + +0.3 349,751 + -1.6 349,781 - -2.7 349,809 - -0.1 349,863 + MMJJ_RS01825 0.15 -0.2 349,863 + MMJJ_RS01825 0.15 -1.0 349,921 + MMJJ_RS01825 0.24 -1.4 349,954 + MMJJ_RS01825 0.29 +0.1 349,954 + MMJJ_RS01825 0.29 +0.4 349,976 - MMJJ_RS01825 0.32 -0.2 350,055 + MMJJ_RS01825 0.44 -0.8 350,093 + MMJJ_RS01825 0.50 -1.0 350,120 - MMJJ_RS01825 0.54 -0.7 350,120 - MMJJ_RS01825 0.54 +0.3 350,121 + MMJJ_RS01825 0.54 +0.4 350,121 + MMJJ_RS01825 0.54 +1.7 350,211 - MMJJ_RS01825 0.68 -2.4
Or see this region's nucleotide sequence