Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01595 and MMJJ_RS01600 are separated by 91 nucleotides MMJJ_RS01600 and MMJJ_RS01605 are separated by 101 nucleotides MMJJ_RS01605 and MMJJ_RS01610 are separated by 75 nucleotides
MMJJ_RS01595: MMJJ_RS01595 - U32 family peptidase, at 310,095 to 311,306
_RS01595
MMJJ_RS01600: MMJJ_RS01600 - radical SAM protein, at 311,398 to 312,291
_RS01600
MMJJ_RS01605: MMJJ_RS01605 - ACT domain-containing protein, at 312,393 to 312,665
_RS01605
MMJJ_RS01610: MMJJ_RS01610 - methanogenesis marker 17 protein, at 312,741 to 313,292
_RS01610
Position (kb) 311
312
313 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 310.512 kb on + strand, within MMJJ_RS01595 at 310.553 kb on + strand, within MMJJ_RS01595 at 310.556 kb on - strand, within MMJJ_RS01595 at 310.568 kb on + strand, within MMJJ_RS01595 at 310.623 kb on - strand, within MMJJ_RS01595 at 310.639 kb on - strand, within MMJJ_RS01595 at 310.672 kb on - strand, within MMJJ_RS01595 at 310.703 kb on + strand, within MMJJ_RS01595 at 310.732 kb on - strand, within MMJJ_RS01595 at 310.733 kb on + strand, within MMJJ_RS01595 at 310.801 kb on - strand, within MMJJ_RS01595 at 310.802 kb on + strand, within MMJJ_RS01595 at 310.852 kb on + strand, within MMJJ_RS01595 at 310.912 kb on + strand, within MMJJ_RS01595 at 310.956 kb on - strand, within MMJJ_RS01595 at 310.957 kb on + strand, within MMJJ_RS01595 at 311.014 kb on - strand, within MMJJ_RS01595 at 311.014 kb on - strand, within MMJJ_RS01595 at 311.019 kb on - strand, within MMJJ_RS01595 at 311.047 kb on + strand, within MMJJ_RS01595 at 311.079 kb on - strand, within MMJJ_RS01595 at 311.162 kb on - strand, within MMJJ_RS01595 at 311.162 kb on - strand, within MMJJ_RS01595 at 311.219 kb on - strand at 311.286 kb on - strand at 311.286 kb on - strand at 311.293 kb on - strand at 311.293 kb on - strand at 311.294 kb on + strand at 311.294 kb on + strand at 311.304 kb on - strand at 311.305 kb on + strand at 311.305 kb on + strand at 311.316 kb on - strand at 311.335 kb on - strand at 311.338 kb on - strand at 311.338 kb on - strand at 311.338 kb on - strand at 311.357 kb on - strand at 311.364 kb on + strand at 311.364 kb on + strand at 311.370 kb on - strand at 311.409 kb on - strand at 311.410 kb on + strand at 311.433 kb on - strand at 311.621 kb on - strand, within MMJJ_RS01600 at 311.775 kb on + strand, within MMJJ_RS01600 at 311.885 kb on + strand, within MMJJ_RS01600 at 311.997 kb on + strand, within MMJJ_RS01600 at 312.094 kb on - strand, within MMJJ_RS01600 at 312.134 kb on - strand, within MMJJ_RS01600 at 312.144 kb on + strand at 312.144 kb on + strand, within MMJJ_RS01600 at 312.216 kb on - strand at 312.217 kb on + strand at 312.249 kb on - strand at 312.265 kb on - strand at 312.554 kb on + strand, within MMJJ_RS01605 at 312.615 kb on + strand, within MMJJ_RS01605 at 312.687 kb on + strand at 312.726 kb on + strand at 313.153 kb on - strand, within MMJJ_RS01610 at 313.193 kb on - strand, within MMJJ_RS01610 at 313.268 kb on - strand at 313.285 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 310,512 + MMJJ_RS01595 0.34 +0.9 310,553 + MMJJ_RS01595 0.38 +0.5 310,556 - MMJJ_RS01595 0.38 +0.6 310,568 + MMJJ_RS01595 0.39 -1.1 310,623 - MMJJ_RS01595 0.44 -0.7 310,639 - MMJJ_RS01595 0.45 +0.5 310,672 - MMJJ_RS01595 0.48 +0.3 310,703 + MMJJ_RS01595 0.50 +1.6 310,732 - MMJJ_RS01595 0.53 +2.7 310,733 + MMJJ_RS01595 0.53 +0.4 310,801 - MMJJ_RS01595 0.58 -1.1 310,802 + MMJJ_RS01595 0.58 -1.2 310,852 + MMJJ_RS01595 0.62 +1.9 310,912 + MMJJ_RS01595 0.67 -0.5 310,956 - MMJJ_RS01595 0.71 -0.2 310,957 + MMJJ_RS01595 0.71 +0.6 311,014 - MMJJ_RS01595 0.76 -1.5 311,014 - MMJJ_RS01595 0.76 -0.9 311,019 - MMJJ_RS01595 0.76 +0.4 311,047 + MMJJ_RS01595 0.79 -1.1 311,079 - MMJJ_RS01595 0.81 +0.6 311,162 - MMJJ_RS01595 0.88 -0.7 311,162 - MMJJ_RS01595 0.88 +0.2 311,219 - -1.7 311,286 - +0.1 311,286 - +0.4 311,293 - +0.4 311,293 - -0.3 311,294 + -1.1 311,294 + +0.4 311,304 - +0.5 311,305 + -1.0 311,305 + -1.4 311,316 - +0.6 311,335 - +1.1 311,338 - +1.8 311,338 - +0.6 311,338 - -0.3 311,357 - +1.4 311,364 + -1.5 311,364 + -0.1 311,370 - +0.1 311,409 - -2.0 311,410 + -2.5 311,433 - -2.9 311,621 - MMJJ_RS01600 0.25 -2.4 311,775 + MMJJ_RS01600 0.42 -2.1 311,885 + MMJJ_RS01600 0.54 -1.7 311,997 + MMJJ_RS01600 0.67 -1.7 312,094 - MMJJ_RS01600 0.78 -1.3 312,134 - MMJJ_RS01600 0.82 -2.6 312,144 + -0.9 312,144 + MMJJ_RS01600 0.83 -2.5 312,216 - +2.2 312,217 + +0.6 312,249 - +0.7 312,265 - -1.3 312,554 + MMJJ_RS01605 0.59 -1.2 312,615 + MMJJ_RS01605 0.81 -1.5 312,687 + -0.7 312,726 + +0.6 313,153 - MMJJ_RS01610 0.75 -2.1 313,193 - MMJJ_RS01610 0.82 -2.1 313,268 - -1.9 313,285 - -1.9
Or see this region's nucleotide sequence