Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00565 and MMJJ_RS00570 are separated by 272 nucleotides MMJJ_RS00570 and MMJJ_RS00575 are separated by 88 nucleotides MMJJ_RS00575 and MMJJ_RS00580 are separated by 22 nucleotides
MMJJ_RS00565: MMJJ_RS00565 - serine--tRNA ligase, at 105,104 to 106,648
_RS00565
MMJJ_RS00570: MMJJ_RS00570 - ribonuclease P, at 106,921 to 107,313
_RS00570
MMJJ_RS00575: MMJJ_RS00575 - carboxylating nicotinate-nucleotide diphosphorylase, at 107,402 to 108,241
_RS00575
MMJJ_RS00580: MMJJ_RS00580 - 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit CofG, at 108,264 to 109,322
_RS00580
Position (kb)
106
107
108 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 106.647 kb on + strand at 106.661 kb on - strand at 106.661 kb on - strand at 106.661 kb on - strand at 106.662 kb on + strand at 106.664 kb on - strand at 106.686 kb on - strand at 106.697 kb on - strand at 106.739 kb on + strand at 106.744 kb on + strand at 106.745 kb on - strand at 106.745 kb on - strand at 106.750 kb on - strand at 106.751 kb on + strand at 106.751 kb on + strand at 106.762 kb on - strand at 106.762 kb on - strand at 106.762 kb on - strand at 106.762 kb on - strand at 106.766 kb on - strand at 106.766 kb on - strand at 106.784 kb on - strand at 106.784 kb on - strand at 106.784 kb on - strand at 106.784 kb on - strand at 106.785 kb on + strand at 106.785 kb on + strand at 106.802 kb on - strand at 106.803 kb on + strand at 106.803 kb on + strand at 106.803 kb on + strand at 106.803 kb on + strand at 106.810 kb on + strand at 106.814 kb on + strand at 106.834 kb on - strand at 106.837 kb on - strand at 106.837 kb on - strand at 106.846 kb on + strand at 106.900 kb on - strand at 106.905 kb on - strand at 106.905 kb on - strand at 106.906 kb on + strand at 106.922 kb on - strand at 106.923 kb on + strand at 107.034 kb on - strand, within MMJJ_RS00570 at 107.035 kb on + strand, within MMJJ_RS00570 at 107.038 kb on - strand, within MMJJ_RS00570 at 107.316 kb on - strand at 107.316 kb on - strand at 107.329 kb on - strand at 107.329 kb on - strand at 107.330 kb on + strand at 107.330 kb on + strand at 107.331 kb on - strand at 107.331 kb on - strand at 107.332 kb on + strand at 107.332 kb on + strand at 107.337 kb on + strand at 107.347 kb on - strand at 107.359 kb on - strand at 107.359 kb on - strand at 107.359 kb on - strand at 107.368 kb on - strand at 107.369 kb on + strand at 107.369 kb on + strand at 107.382 kb on + strand at 107.383 kb on - strand at 107.454 kb on - strand at 108.022 kb on + strand at 108.054 kb on - strand, within MMJJ_RS00575 at 108.066 kb on - strand, within MMJJ_RS00575 at 108.142 kb on - strand, within MMJJ_RS00575 at 108.179 kb on + strand at 108.239 kb on - strand at 108.264 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 106,647 + -0.6 106,661 - +0.9 106,661 - +1.7 106,661 - +0.9 106,662 + -1.3 106,664 - -1.0 106,686 - +2.4 106,697 - +0.6 106,739 + -0.2 106,744 + +1.0 106,745 - -0.3 106,745 - +0.7 106,750 - +0.4 106,751 + +0.5 106,751 + -1.2 106,762 - +0.4 106,762 - +1.0 106,762 - +0.2 106,762 - -0.3 106,766 - +0.6 106,766 - +1.2 106,784 - +1.0 106,784 - +1.6 106,784 - -1.2 106,784 - +0.5 106,785 + +0.4 106,785 + -0.4 106,802 - -1.0 106,803 + +1.0 106,803 + +0.7 106,803 + +0.2 106,803 + -0.2 106,810 + -0.7 106,814 + -0.5 106,834 - +0.5 106,837 - -0.5 106,837 - +0.8 106,846 + -0.3 106,900 - +0.1 106,905 - +0.6 106,905 - -2.5 106,906 + -1.3 106,922 - +0.8 106,923 + +0.5 107,034 - MMJJ_RS00570 0.29 -3.0 107,035 + MMJJ_RS00570 0.29 -1.6 107,038 - MMJJ_RS00570 0.30 -3.1 107,316 - -2.2 107,316 - -2.2 107,329 - -0.1 107,329 - +1.1 107,330 + +1.0 107,330 + +1.1 107,331 - -1.5 107,331 - -1.1 107,332 + +0.4 107,332 + -3.4 107,337 + +0.3 107,347 - +0.6 107,359 - -0.6 107,359 - -1.5 107,359 - -3.6 107,368 - +0.7 107,369 + -0.9 107,369 + -0.9 107,382 + +0.1 107,383 - -1.6 107,454 - -3.8 108,022 + -1.5 108,054 - MMJJ_RS00575 0.78 +0.1 108,066 - MMJJ_RS00575 0.79 -2.9 108,142 - MMJJ_RS00575 0.88 -1.1 108,179 + -1.2 108,239 - -0.1 108,264 + -2.1
Or see this region's nucleotide sequence