Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00265 and MMJJ_RS00270 are separated by 15 nucleotides MMJJ_RS00270 and MMJJ_RS00275 are separated by 53 nucleotides MMJJ_RS00275 and MMJJ_RS00280 are separated by 16 nucleotides MMJJ_RS00280 and MMJJ_RS00285 are separated by 5 nucleotides
MMJJ_RS00265: MMJJ_RS00265 - TIGR02253 family HAD-type hydrolase, at 51,811 to 52,488
_RS00265
MMJJ_RS00270: MMJJ_RS00270 - adenosylcobinamide-GDP ribazoletransferase, at 52,504 to 53,277
_RS00270
MMJJ_RS00275: MMJJ_RS00275 - coenzyme F420-0:L-glutamate ligase, at 53,331 to 54,077
_RS00275
MMJJ_RS00280: MMJJ_RS00280 - shikimate dehydrogenase, at 54,094 to 54,930
_RS00280
MMJJ_RS00285: MMJJ_RS00285 - UPF0254 family protein, at 54,936 to 55,433
_RS00285
Position (kb) 53
54
55 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 52.427 kb on - strand at 52.428 kb on + strand at 52.452 kb on + strand at 52.482 kb on - strand at 52.654 kb on + strand, within MMJJ_RS00270 at 52.655 kb on - strand, within MMJJ_RS00270 at 52.719 kb on + strand, within MMJJ_RS00270 at 52.723 kb on + strand, within MMJJ_RS00270 at 52.810 kb on + strand, within MMJJ_RS00270 at 52.810 kb on + strand, within MMJJ_RS00270 at 52.928 kb on + strand, within MMJJ_RS00270 at 52.934 kb on - strand, within MMJJ_RS00270 at 53.082 kb on + strand at 53.257 kb on - strand at 53.269 kb on + strand at 53.279 kb on - strand at 53.300 kb on + strand at 53.305 kb on - strand at 53.309 kb on - strand at 53.561 kb on - strand, within MMJJ_RS00275 at 53.667 kb on + strand, within MMJJ_RS00275 at 53.838 kb on - strand, within MMJJ_RS00275 at 53.839 kb on + strand, within MMJJ_RS00275 at 53.981 kb on + strand, within MMJJ_RS00275 at 54.006 kb on + strand at 54.108 kb on - strand at 54.130 kb on - strand at 54.131 kb on + strand at 54.298 kb on + strand, within MMJJ_RS00280 at 54.495 kb on - strand, within MMJJ_RS00280 at 54.862 kb on - strand at 54.943 kb on + strand at 54.950 kb on + strand at 54.977 kb on - strand at 54.977 kb on - strand at 54.977 kb on - strand at 54.982 kb on - strand at 54.993 kb on - strand, within MMJJ_RS00285 at 55.018 kb on + strand, within MMJJ_RS00285 at 55.033 kb on + strand, within MMJJ_RS00285 at 55.035 kb on - strand, within MMJJ_RS00285 at 55.035 kb on - strand, within MMJJ_RS00285 at 55.036 kb on + strand, within MMJJ_RS00285 at 55.036 kb on + strand, within MMJJ_RS00285 at 55.067 kb on + strand, within MMJJ_RS00285 at 55.076 kb on + strand, within MMJJ_RS00285
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 52,427 - -0.4 52,428 + +0.3 52,452 + -1.7 52,482 - -0.3 52,654 + MMJJ_RS00270 0.19 -2.2 52,655 - MMJJ_RS00270 0.20 -2.8 52,719 + MMJJ_RS00270 0.28 -2.6 52,723 + MMJJ_RS00270 0.28 -2.4 52,810 + MMJJ_RS00270 0.40 -0.9 52,810 + MMJJ_RS00270 0.40 -3.1 52,928 + MMJJ_RS00270 0.55 -1.3 52,934 - MMJJ_RS00270 0.56 -1.7 53,082 + -0.5 53,257 - +0.8 53,269 + -0.5 53,279 - -0.6 53,300 + +0.4 53,305 - +1.1 53,309 - -0.2 53,561 - MMJJ_RS00275 0.31 -2.6 53,667 + MMJJ_RS00275 0.45 -3.5 53,838 - MMJJ_RS00275 0.68 -1.1 53,839 + MMJJ_RS00275 0.68 -1.5 53,981 + MMJJ_RS00275 0.87 -3.2 54,006 + -1.2 54,108 - +0.7 54,130 - -3.4 54,131 + -1.7 54,298 + MMJJ_RS00280 0.24 -2.3 54,495 - MMJJ_RS00280 0.48 -0.7 54,862 - -1.2 54,943 + -0.8 54,950 + -0.5 54,977 - -3.3 54,977 - -1.8 54,977 - -0.4 54,982 - -2.0 54,993 - MMJJ_RS00285 0.11 -1.8 55,018 + MMJJ_RS00285 0.16 -2.9 55,033 + MMJJ_RS00285 0.19 -0.6 55,035 - MMJJ_RS00285 0.20 -0.8 55,035 - MMJJ_RS00285 0.20 -1.3 55,036 + MMJJ_RS00285 0.20 -4.0 55,036 + MMJJ_RS00285 0.20 -0.8 55,067 + MMJJ_RS00285 0.26 -3.3 55,076 + MMJJ_RS00285 0.28 -3.4
Or see this region's nucleotide sequence