Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00205 and MMJJ_RS00210 are separated by 10 nucleotides MMJJ_RS00210 and MMJJ_RS00215 are separated by 12 nucleotides
MMJJ_RS00205: MMJJ_RS00205 - adenosylcobinamide-phosphate synthase CbiB, at 38,646 to 39,566
_RS00205
MMJJ_RS00210: MMJJ_RS00210 - DUF530 family protein, at 39,577 to 41,007
_RS00210
MMJJ_RS00215: MMJJ_RS00215 - CCA tRNA nucleotidyltransferase, at 41,020 to 42,354
_RS00215
Position (kb) 39
40
41
42 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 38.580 kb on - strand at 38.665 kb on - strand at 38.665 kb on - strand at 38.755 kb on - strand, within MMJJ_RS00205 at 38.758 kb on - strand, within MMJJ_RS00205 at 38.780 kb on - strand, within MMJJ_RS00205 at 38.784 kb on - strand, within MMJJ_RS00205 at 38.903 kb on - strand, within MMJJ_RS00205 at 38.983 kb on + strand, within MMJJ_RS00205 at 39.090 kb on + strand, within MMJJ_RS00205 at 39.153 kb on - strand, within MMJJ_RS00205 at 39.165 kb on + strand, within MMJJ_RS00205 at 39.175 kb on + strand, within MMJJ_RS00205 at 39.175 kb on + strand, within MMJJ_RS00205 at 39.175 kb on + strand, within MMJJ_RS00205 at 39.180 kb on + strand, within MMJJ_RS00205 at 39.180 kb on + strand, within MMJJ_RS00205 at 39.309 kb on + strand, within MMJJ_RS00205 at 39.777 kb on + strand, within MMJJ_RS00210 at 40.416 kb on - strand at 40.450 kb on - strand at 40.490 kb on + strand, within MMJJ_RS00210 at 40.601 kb on - strand, within MMJJ_RS00210 at 40.759 kb on + strand, within MMJJ_RS00210 at 40.769 kb on - strand, within MMJJ_RS00210 at 41.021 kb on - strand at 41.041 kb on + strand at 41.041 kb on + strand at 41.045 kb on + strand at 41.045 kb on + strand at 41.072 kb on + strand at 41.076 kb on + strand at 41.238 kb on + strand, within MMJJ_RS00215 at 41.502 kb on + strand, within MMJJ_RS00215 at 41.570 kb on + strand, within MMJJ_RS00215 at 41.679 kb on + strand, within MMJJ_RS00215 at 41.875 kb on - strand, within MMJJ_RS00215
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 38,580 - +0.5 38,665 - -1.7 38,665 - -0.9 38,755 - MMJJ_RS00205 0.12 -2.5 38,758 - MMJJ_RS00205 0.12 -0.4 38,780 - MMJJ_RS00205 0.15 -2.3 38,784 - MMJJ_RS00205 0.15 -0.2 38,903 - MMJJ_RS00205 0.28 -1.2 38,983 + MMJJ_RS00205 0.37 -4.2 39,090 + MMJJ_RS00205 0.48 -0.2 39,153 - MMJJ_RS00205 0.55 -1.5 39,165 + MMJJ_RS00205 0.56 -1.7 39,175 + MMJJ_RS00205 0.57 +1.1 39,175 + MMJJ_RS00205 0.57 -1.5 39,175 + MMJJ_RS00205 0.57 -3.5 39,180 + MMJJ_RS00205 0.58 -0.9 39,180 + MMJJ_RS00205 0.58 -1.9 39,309 + MMJJ_RS00205 0.72 -0.5 39,777 + MMJJ_RS00210 0.14 -1.5 40,416 - -0.5 40,450 - +1.4 40,490 + MMJJ_RS00210 0.64 -2.5 40,601 - MMJJ_RS00210 0.72 -2.5 40,759 + MMJJ_RS00210 0.83 -2.1 40,769 - MMJJ_RS00210 0.83 -3.3 41,021 - -1.3 41,041 + +0.4 41,041 + +1.1 41,045 + +0.3 41,045 + +0.1 41,072 + -1.0 41,076 + -1.2 41,238 + MMJJ_RS00215 0.16 -2.1 41,502 + MMJJ_RS00215 0.36 -2.5 41,570 + MMJJ_RS00215 0.41 -0.5 41,679 + MMJJ_RS00215 0.49 -0.9 41,875 - MMJJ_RS00215 0.64 -0.2
Or see this region's nucleotide sequence