Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05030 and H281DRAFT_05031 are separated by 107 nucleotides H281DRAFT_05031 and H281DRAFT_05032 are separated by 22 nucleotides H281DRAFT_05032 and H281DRAFT_05033 are separated by 149 nucleotides H281DRAFT_05033 and H281DRAFT_05034 are separated by 230 nucleotides
H281DRAFT_05030: H281DRAFT_05030 - transcriptional regulator, TetR family, at 219,102 to 219,689
_05030
H281DRAFT_05031: H281DRAFT_05031 - Cytochrome P460, at 219,797 to 220,306
_05031
H281DRAFT_05032: H281DRAFT_05032 - Pimeloyl-ACP methyl ester carboxylesterase, at 220,329 to 221,360
_05032
H281DRAFT_05033: H281DRAFT_05033 - protein of unknown function (DUF4148), at 221,510 to 221,929
_05033
H281DRAFT_05034: H281DRAFT_05034 - hypothetical protein, at 222,160 to 223,359
_05034
Position (kb)
220
221
222 Strain fitness (log2 ratio)
-1
0
1
2 at 220.426 kb on + strand at 220.427 kb on - strand at 220.691 kb on + strand, within H281DRAFT_05032 at 220.837 kb on + strand, within H281DRAFT_05032 at 221.109 kb on - strand, within H281DRAFT_05032 at 221.109 kb on - strand, within H281DRAFT_05032 at 221.111 kb on - strand, within H281DRAFT_05032 at 221.111 kb on - strand, within H281DRAFT_05032 at 221.111 kb on - strand, within H281DRAFT_05032 at 221.111 kb on - strand, within H281DRAFT_05032 at 221.111 kb on - strand, within H281DRAFT_05032 at 221.365 kb on - strand at 221.365 kb on - strand at 221.365 kb on - strand at 221.477 kb on - strand at 221.523 kb on + strand at 221.638 kb on + strand, within H281DRAFT_05033 at 221.670 kb on + strand at 221.983 kb on + strand at 221.987 kb on + strand at 221.988 kb on - strand at 222.017 kb on - strand at 222.328 kb on + strand, within H281DRAFT_05034 at 222.329 kb on - strand, within H281DRAFT_05034
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x remove 220,426 + -0.0 220,427 - +0.6 220,691 + H281DRAFT_05032 0.35 -0.4 220,837 + H281DRAFT_05032 0.49 -0.1 221,109 - H281DRAFT_05032 0.76 -0.0 221,109 - H281DRAFT_05032 0.76 -1.2 221,111 - H281DRAFT_05032 0.76 +0.1 221,111 - H281DRAFT_05032 0.76 -1.2 221,111 - H281DRAFT_05032 0.76 -1.3 221,111 - H281DRAFT_05032 0.76 +0.5 221,111 - H281DRAFT_05032 0.76 +0.1 221,365 - +2.4 221,365 - -0.2 221,365 - -0.1 221,477 - +0.3 221,523 + -0.1 221,638 + H281DRAFT_05033 0.30 +0.4 221,670 + -0.2 221,983 + +0.8 221,987 + -0.8 221,988 - +0.3 222,017 - +0.8 222,328 + H281DRAFT_05034 0.14 -1.4 222,329 - H281DRAFT_05034 0.14 +0.5
Or see this region's nucleotide sequence