Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03135 and BPHYT_RS03140 overlap by 4 nucleotides BPHYT_RS03140 and BPHYT_RS03145 are separated by 23 nucleotides BPHYT_RS03145 and BPHYT_RS03150 are separated by 504 nucleotides
BPHYT_RS03135: BPHYT_RS03135 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 701,210 to 702,034
_RS03135
BPHYT_RS03140: BPHYT_RS03140 - 5'-nucleotidase, at 702,031 to 702,933
_RS03140
BPHYT_RS03145: BPHYT_RS03145 - threonine dehydratase, at 702,957 to 704,480
_RS03145
BPHYT_RS03150: BPHYT_RS03150 - Phosphoglycerate dehydrogenase (EC:1.1.1.95) <small>(from data)</small>, at 704,985 to 709,079
_RS03150
Position (kb) 702
703
704
705 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 701.982 kb on + strand at 701.983 kb on - strand at 702.265 kb on - strand, within BPHYT_RS03140 at 702.270 kb on + strand, within BPHYT_RS03140 at 702.270 kb on + strand, within BPHYT_RS03140 at 702.270 kb on + strand, within BPHYT_RS03140 at 702.271 kb on - strand, within BPHYT_RS03140 at 702.271 kb on - strand, within BPHYT_RS03140 at 702.271 kb on - strand, within BPHYT_RS03140 at 702.631 kb on + strand, within BPHYT_RS03140 at 702.632 kb on - strand, within BPHYT_RS03140 at 702.828 kb on - strand, within BPHYT_RS03140 at 702.851 kb on + strand at 702.954 kb on + strand at 702.954 kb on + strand at 702.955 kb on - strand at 702.955 kb on - strand at 704.539 kb on + strand at 704.540 kb on - strand at 704.708 kb on - strand at 704.830 kb on + strand at 704.835 kb on - strand at 704.835 kb on - strand at 704.835 kb on - strand at 704.835 kb on - strand at 704.836 kb on + strand at 704.837 kb on - strand at 704.837 kb on - strand at 704.895 kb on + strand at 704.968 kb on + strand at 704.968 kb on + strand at 705.075 kb on + strand at 705.076 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 701,982 + +0.4 701,983 - -0.1 702,265 - BPHYT_RS03140 0.26 -0.2 702,270 + BPHYT_RS03140 0.26 +0.1 702,270 + BPHYT_RS03140 0.26 -0.2 702,270 + BPHYT_RS03140 0.26 -2.3 702,271 - BPHYT_RS03140 0.27 +0.3 702,271 - BPHYT_RS03140 0.27 -0.0 702,271 - BPHYT_RS03140 0.27 -0.0 702,631 + BPHYT_RS03140 0.66 +1.5 702,632 - BPHYT_RS03140 0.67 -0.2 702,828 - BPHYT_RS03140 0.88 -1.6 702,851 + +0.1 702,954 + +0.5 702,954 + +0.3 702,955 - +1.2 702,955 - +0.1 704,539 + -0.1 704,540 - -1.0 704,708 - -0.3 704,830 + -0.7 704,835 - -4.7 704,835 - -3.2 704,835 - -1.1 704,835 - -5.8 704,836 + -0.2 704,837 - -3.7 704,837 - -4.5 704,895 + -0.0 704,968 + -0.0 704,968 + -0.0 705,075 + -5.1 705,076 - -2.2
Or see this region's nucleotide sequence