Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_03420

Experiment: Chloramphenicol 0.0025 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_03415 and AO353_03420 overlap by 4 nucleotidesAO353_03420 and AO353_03425 are separated by 224 nucleotides AO353_03415: AO353_03415 - glucokinase, at 727,854 to 728,804 _03415 AO353_03420: AO353_03420 - phosphogluconate dehydratase, at 728,801 to 730,627 _03420 AO353_03425: AO353_03425 - glyceraldehyde-3-phosphate dehydrogenase, at 730,852 to 731,853 _03425 Position (kb) 728 729 730 731Strain fitness (log2 ratio) -2 -1 0 1 2at 727.852 kb on + strandat 727.852 kb on + strandat 727.852 kb on + strandat 727.852 kb on + strandat 727.897 kb on + strandat 727.898 kb on - strandat 727.936 kb on + strandat 727.936 kb on + strandat 727.936 kb on + strandat 727.937 kb on - strandat 727.937 kb on - strandat 727.937 kb on - strandat 727.937 kb on - strandat 727.937 kb on - strandat 727.937 kb on - strandat 727.937 kb on - strandat 728.029 kb on + strandat 728.029 kb on + strand, within AO353_03415at 728.029 kb on + strand, within AO353_03415at 728.029 kb on + strand, within AO353_03415at 728.029 kb on + strand, within AO353_03415at 728.029 kb on + strand, within AO353_03415at 728.029 kb on + strand, within AO353_03415at 728.029 kb on + strand, within AO353_03415at 728.030 kb on - strand, within AO353_03415at 728.030 kb on - strand, within AO353_03415at 728.030 kb on - strand, within AO353_03415at 728.030 kb on - strand, within AO353_03415at 728.030 kb on - strand, within AO353_03415at 728.030 kb on - strand, within AO353_03415at 728.030 kb on - strand, within AO353_03415at 728.207 kb on - strand, within AO353_03415at 728.269 kb on + strand, within AO353_03415at 728.269 kb on + strand, within AO353_03415at 728.270 kb on - strand, within AO353_03415at 728.270 kb on - strand, within AO353_03415at 728.270 kb on - strand, within AO353_03415at 728.270 kb on - strand, within AO353_03415at 728.582 kb on + strand, within AO353_03415at 728.582 kb on + strand, within AO353_03415at 728.671 kb on + strand, within AO353_03415at 728.671 kb on + strand, within AO353_03415at 728.672 kb on - strand, within AO353_03415at 728.701 kb on + strandat 728.701 kb on + strand, within AO353_03415at 728.701 kb on + strand, within AO353_03415at 728.701 kb on + strand, within AO353_03415at 728.796 kb on + strandat 728.797 kb on - strandat 728.797 kb on - strandat 728.797 kb on - strandat 728.797 kb on - strandat 728.797 kb on - strandat 728.806 kb on + strandat 728.806 kb on + strandat 728.806 kb on + strandat 728.806 kb on + strandat 728.807 kb on - strandat 728.807 kb on - strandat 728.807 kb on - strandat 728.827 kb on + strandat 729.957 kb on + strand, within AO353_03420at 729.958 kb on - strand, within AO353_03420at 729.964 kb on + strand, within AO353_03420at 729.964 kb on + strand, within AO353_03420at 729.964 kb on + strand, within AO353_03420at 729.964 kb on + strand, within AO353_03420at 729.965 kb on - strand, within AO353_03420at 729.985 kb on - strand, within AO353_03420at 730.039 kb on - strand, within AO353_03420at 730.405 kb on + strand, within AO353_03420at 730.405 kb on + strand, within AO353_03420at 730.405 kb on + strand, within AO353_03420at 730.406 kb on - strand, within AO353_03420at 730.406 kb on - strand, within AO353_03420at 730.406 kb on - strand, within AO353_03420at 730.408 kb on - strand, within AO353_03420at 730.408 kb on - strand, within AO353_03420at 730.561 kb on - strandat 730.631 kb on + strandat 730.631 kb on + strandat 730.631 kb on + strandat 730.631 kb on + strandat 730.632 kb on - strandat 730.632 kb on - strandat 730.632 kb on - strandat 730.632 kb on - strandat 730.671 kb on + strandat 730.672 kb on - strandat 730.672 kb on - strandat 730.758 kb on - strandat 730.851 kb on + strandat 730.851 kb on + strandat 730.852 kb on - strandat 730.852 kb on - strandat 730.860 kb on - strandat 730.860 kb on - strandat 730.915 kb on + strandat 730.915 kb on + strandat 730.915 kb on + strandat 730.915 kb on + strandat 730.915 kb on + strandat 730.915 kb on + strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.918 kb on - strandat 730.941 kb on - strandat 730.941 kb on - strandat 730.971 kb on + strand, within AO353_03425at 730.999 kb on + strand, within AO353_03425at 730.999 kb on + strand, within AO353_03425at 730.999 kb on + strand, within AO353_03425at 730.999 kb on + strand, within AO353_03425at 730.999 kb on + strand, within AO353_03425at 730.999 kb on + strand, within AO353_03425at 731.000 kb on - strand, within AO353_03425at 731.000 kb on - strand, within AO353_03425at 731.000 kb on - strand, within AO353_03425at 731.188 kb on - strand, within AO353_03425at 731.188 kb on - strand, within AO353_03425at 731.188 kb on - strand, within AO353_03425at 731.188 kb on - strand, within AO353_03425at 731.188 kb on - strand, within AO353_03425at 731.188 kb on - strand, within AO353_03425at 731.188 kb on - strand, within AO353_03425at 731.190 kb on + strand, within AO353_03425at 731.214 kb on + strand, within AO353_03425at 731.214 kb on + strand, within AO353_03425at 731.215 kb on - strand, within AO353_03425at 731.215 kb on - strandat 731.254 kb on + strand, within AO353_03425at 731.398 kb on + strand, within AO353_03425at 731.398 kb on + strand, within AO353_03425at 731.398 kb on + strand, within AO353_03425at 731.398 kb on + strand, within AO353_03425at 731.398 kb on + strand, within AO353_03425at 731.398 kb on + strand, within AO353_03425at 731.399 kb on - strand, within AO353_03425at 731.399 kb on - strand, within AO353_03425at 731.399 kb on - strand, within AO353_03425at 731.418 kb on + strand, within AO353_03425at 731.419 kb on - strand, within AO353_03425at 731.419 kb on - strand, within AO353_03425at 731.428 kb on + strand, within AO353_03425

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloramphenicol 0.0025 mg/ml
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727,852 + -1.5
727,852 + -0.2
727,852 + -0.6
727,852 + -0.6
727,897 + +0.1
727,898 - -0.3
727,936 + +0.4
727,936 + -0.3
727,936 + -1.6
727,937 - +0.2
727,937 - -1.6
727,937 - -0.5
727,937 - +0.7
727,937 - +0.3
727,937 - +1.2
727,937 - +1.6
728,029 + -0.1
728,029 + AO353_03415 0.18 -0.1
728,029 + AO353_03415 0.18 +0.6
728,029 + AO353_03415 0.18 -1.1
728,029 + AO353_03415 0.18 -0.7
728,029 + AO353_03415 0.18 +0.4
728,029 + AO353_03415 0.18 +0.3
728,029 + AO353_03415 0.18 +0.0
728,030 - AO353_03415 0.19 -1.5
728,030 - AO353_03415 0.19 -0.1
728,030 - AO353_03415 0.19 -0.7
728,030 - AO353_03415 0.19 -0.4
728,030 - AO353_03415 0.19 -0.4
728,030 - AO353_03415 0.19 -0.7
728,030 - AO353_03415 0.19 -0.3
728,207 - AO353_03415 0.37 +0.3
728,269 + AO353_03415 0.44 -0.1
728,269 + AO353_03415 0.44 +0.6
728,270 - AO353_03415 0.44 -0.1
728,270 - AO353_03415 0.44 -0.3
728,270 - AO353_03415 0.44 +0.4
728,270 - AO353_03415 0.44 -0.1
728,582 + AO353_03415 0.77 -0.3
728,582 + AO353_03415 0.77 +0.3
728,671 + AO353_03415 0.86 -0.5
728,671 + AO353_03415 0.86 -1.6
728,672 - AO353_03415 0.86 -1.0
728,701 + +1.9
728,701 + AO353_03415 0.89 -1.7
728,701 + AO353_03415 0.89 +0.3
728,701 + AO353_03415 0.89 -1.0
728,796 + +0.5
728,797 - -1.3
728,797 - -0.8
728,797 - +0.5
728,797 - -0.3
728,797 - +1.0
728,806 + -0.0
728,806 + -2.1
728,806 + +0.9
728,806 + -1.0
728,807 - -0.3
728,807 - +0.3
728,807 - +0.9
728,827 + -2.2
729,957 + AO353_03420 0.63 +0.1
729,958 - AO353_03420 0.63 -1.9
729,964 + AO353_03420 0.64 -0.8
729,964 + AO353_03420 0.64 -0.9
729,964 + AO353_03420 0.64 -0.8
729,964 + AO353_03420 0.64 -0.9
729,965 - AO353_03420 0.64 -0.1
729,985 - AO353_03420 0.65 +1.3
730,039 - AO353_03420 0.68 +1.3
730,405 + AO353_03420 0.88 +0.9
730,405 + AO353_03420 0.88 -0.2
730,405 + AO353_03420 0.88 -1.8
730,406 - AO353_03420 0.88 -0.9
730,406 - AO353_03420 0.88 -0.8
730,406 - AO353_03420 0.88 +0.4
730,408 - AO353_03420 0.88 -0.7
730,408 - AO353_03420 0.88 -0.5
730,561 - -1.1
730,631 + -1.3
730,631 + -0.5
730,631 + -1.7
730,631 + -1.3
730,632 - -0.9
730,632 - +0.9
730,632 - -1.2
730,632 - -2.5
730,671 + +0.6
730,672 - -0.3
730,672 - -0.0
730,758 - +0.0
730,851 + -0.3
730,851 + +1.6
730,852 - -1.0
730,852 - -0.5
730,860 - +0.3
730,860 - -0.1
730,915 + +0.6
730,915 + +1.9
730,915 + -0.0
730,915 + +1.1
730,915 + +0.1
730,915 + +0.3
730,916 - -0.4
730,916 - +1.3
730,916 - +2.6
730,916 - +0.5
730,916 - -0.5
730,916 - +1.1
730,916 - +0.9
730,916 - -1.0
730,916 - -0.1
730,916 - +0.5
730,918 - -1.3
730,941 - -0.2
730,941 - -0.5
730,971 + AO353_03425 0.12 -0.2
730,999 + AO353_03425 0.15 -0.7
730,999 + AO353_03425 0.15 +0.4
730,999 + AO353_03425 0.15 -1.7
730,999 + AO353_03425 0.15 -1.0
730,999 + AO353_03425 0.15 -0.3
730,999 + AO353_03425 0.15 -1.1
731,000 - AO353_03425 0.15 -0.1
731,000 - AO353_03425 0.15 -1.3
731,000 - AO353_03425 0.15 -0.1
731,188 - AO353_03425 0.34 -0.7
731,188 - AO353_03425 0.34 +0.0
731,188 - AO353_03425 0.34 -0.8
731,188 - AO353_03425 0.34 -0.1
731,188 - AO353_03425 0.34 +1.0
731,188 - AO353_03425 0.34 -0.6
731,188 - AO353_03425 0.34 -0.6
731,190 + AO353_03425 0.34 -0.9
731,214 + AO353_03425 0.36 +0.0
731,214 + AO353_03425 0.36 +0.9
731,215 - AO353_03425 0.36 +0.4
731,215 - +0.9
731,254 + AO353_03425 0.40 +0.8
731,398 + AO353_03425 0.54 +0.1
731,398 + AO353_03425 0.54 +0.9
731,398 + AO353_03425 0.54 -0.1
731,398 + AO353_03425 0.54 +1.4
731,398 + AO353_03425 0.54 -0.7
731,398 + AO353_03425 0.54 +0.7
731,399 - AO353_03425 0.55 -0.5
731,399 - AO353_03425 0.55 -0.7
731,399 - AO353_03425 0.55 -0.0
731,418 + AO353_03425 0.56 +0.4
731,419 - AO353_03425 0.57 -0.6
731,419 - AO353_03425 0.57 -0.3
731,428 + AO353_03425 0.57 +0.3

Or see this region's nucleotide sequence