Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1423 and DvMF_1424 are separated by 321 nucleotides DvMF_1424 and pyrB are separated by 14 nucleotides pyrB and DvMF_1426 overlap by 4 nucleotides
DvMF_1423: DvMF_1423 - hypothetical protein (RefSeq), at 1,739,952 to 1,740,854
_1423
DvMF_1424: DvMF_1424 - amidohydrolase (RefSeq), at 1,741,176 to 1,742,612
_1424
DvMF_1425: pyrB - aspartate carbamoyltransferase catalytic subunit (RefSeq), at 1,742,627 to 1,743,604
pyrB
DvMF_1426: DvMF_1426 - dihydroorotase, multifunctional complex type (RefSeq), at 1,743,601 to 1,744,881
_1426
Position (kb)
1741
1742
1743 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1740.178 kb on + strand, within DvMF_1423 at 1740.247 kb on - strand, within DvMF_1423 at 1740.358 kb on + strand, within DvMF_1423 at 1740.358 kb on + strand, within DvMF_1423 at 1740.358 kb on + strand, within DvMF_1423 at 1740.489 kb on + strand, within DvMF_1423 at 1740.570 kb on - strand, within DvMF_1423 at 1740.813 kb on - strand at 1740.844 kb on + strand at 1740.849 kb on + strand at 1740.868 kb on + strand at 1740.970 kb on + strand at 1741.119 kb on + strand at 1742.691 kb on + strand at 1742.777 kb on - strand, within pyrB at 1742.886 kb on + strand, within pyrB at 1742.904 kb on - strand, within pyrB at 1743.023 kb on - strand, within pyrB at 1743.083 kb on - strand, within pyrB at 1743.091 kb on + strand, within pyrB at 1743.151 kb on - strand, within pyrB at 1743.176 kb on - strand, within pyrB at 1743.200 kb on - strand, within pyrB at 1743.268 kb on - strand, within pyrB at 1743.441 kb on - strand, within pyrB at 1743.492 kb on + strand, within pyrB at 1743.542 kb on - strand at 1743.575 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 1,740,178 + DvMF_1423 0.25 -0.3 1,740,247 - DvMF_1423 0.33 -0.2 1,740,358 + DvMF_1423 0.45 +0.1 1,740,358 + DvMF_1423 0.45 +0.3 1,740,358 + DvMF_1423 0.45 -0.0 1,740,489 + DvMF_1423 0.59 +0.0 1,740,570 - DvMF_1423 0.68 -0.0 1,740,813 - -0.3 1,740,844 + -0.6 1,740,849 + +0.0 1,740,868 + -0.3 1,740,970 + -0.2 1,741,119 + -0.9 1,742,691 + -4.1 1,742,777 - pyrB DvMF_1425 0.15 -5.7 1,742,886 + pyrB DvMF_1425 0.26 -5.1 1,742,904 - pyrB DvMF_1425 0.28 -3.4 1,743,023 - pyrB DvMF_1425 0.40 -4.5 1,743,083 - pyrB DvMF_1425 0.47 -2.7 1,743,091 + pyrB DvMF_1425 0.47 -4.3 1,743,151 - pyrB DvMF_1425 0.54 -4.3 1,743,176 - pyrB DvMF_1425 0.56 -7.0 1,743,200 - pyrB DvMF_1425 0.59 -3.4 1,743,268 - pyrB DvMF_1425 0.66 -3.8 1,743,441 - pyrB DvMF_1425 0.83 -4.3 1,743,492 + pyrB DvMF_1425 0.88 -5.0 1,743,542 - -4.7 1,743,575 - -0.1
Or see this region's nucleotide sequence