Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0798 and thiH are separated by 84 nucleotides thiH and thiG are separated by 119 nucleotides thiG and DvMF_0801 overlap by 1 nucleotides
DvMF_0798: DvMF_0798 - thiamine biosynthesis protein ThiF (RefSeq), at 962,598 to 963,218
_0798
DvMF_0799: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 963,303 to 964,547
thiH
DvMF_0800: thiG - thiazole synthase (RefSeq), at 964,667 to 965,467
thiG
DvMF_0801: DvMF_0801 - thiamine biosynthesis protein ThiS (RefSeq), at 965,467 to 965,667
_0801
Position (kb) 963
964
965 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 962.417 kb on - strand at 962.504 kb on + strand at 962.583 kb on - strand at 962.859 kb on + strand, within DvMF_0798 at 963.068 kb on + strand, within DvMF_0798 at 963.186 kb on - strand at 963.734 kb on + strand, within thiH at 963.885 kb on + strand, within thiH at 963.893 kb on - strand, within thiH at 963.967 kb on + strand, within thiH at 963.980 kb on + strand, within thiH at 964.032 kb on - strand, within thiH at 964.065 kb on - strand, within thiH at 964.590 kb on - strand at 964.801 kb on - strand, within thiG at 964.877 kb on - strand, within thiG at 965.230 kb on + strand, within thiG at 965.238 kb on + strand, within thiG at 965.297 kb on + strand, within thiG at 965.317 kb on + strand, within thiG at 965.348 kb on - strand, within thiG at 965.356 kb on + strand, within thiG at 965.356 kb on + strand, within thiG at 965.493 kb on - strand, within DvMF_0801 at 965.525 kb on + strand, within DvMF_0801 at 965.542 kb on - strand, within DvMF_0801
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 962,417 - +0.0 962,504 + -0.4 962,583 - -0.2 962,859 + DvMF_0798 0.42 -2.7 963,068 + DvMF_0798 0.76 -2.8 963,186 - -3.5 963,734 + thiH DvMF_0799 0.35 -3.7 963,885 + thiH DvMF_0799 0.47 -3.5 963,893 - thiH DvMF_0799 0.47 -2.8 963,967 + thiH DvMF_0799 0.53 -2.6 963,980 + thiH DvMF_0799 0.54 -2.2 964,032 - thiH DvMF_0799 0.59 -2.9 964,065 - thiH DvMF_0799 0.61 -2.6 964,590 - -0.4 964,801 - thiG DvMF_0800 0.17 -3.0 964,877 - thiG DvMF_0800 0.26 -2.6 965,230 + thiG DvMF_0800 0.70 -2.7 965,238 + thiG DvMF_0800 0.71 -2.6 965,297 + thiG DvMF_0800 0.79 -2.5 965,317 + thiG DvMF_0800 0.81 -2.9 965,348 - thiG DvMF_0800 0.85 -5.1 965,356 + thiG DvMF_0800 0.86 -3.5 965,356 + thiG DvMF_0800 0.86 -4.4 965,493 - DvMF_0801 0.13 -3.5 965,525 + DvMF_0801 0.29 -3.9 965,542 - DvMF_0801 0.37 -3.2
Or see this region's nucleotide sequence