Experiment: BHIS with sodium fluoride 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1940 and BT1941 are separated by 268 nucleotides BT1941 and BT1942 are separated by 31 nucleotides
BT1940: BT1940 - hypothetical protein (NCBI ptt file), at 2,441,541 to 2,443,151
BT1940
BT1941: BT1941 - hypothetical protein (NCBI ptt file), at 2,443,420 to 2,444,250
BT1941
BT1942: BT1942 - hypothetical protein (NCBI ptt file), at 2,444,282 to 2,444,722
BT1942
Position (kb)
2443
2444
2445 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2442.425 kb on + strand, within BT1940 at 2442.425 kb on + strand, within BT1940 at 2442.426 kb on - strand, within BT1940 at 2442.436 kb on - strand, within BT1940 at 2442.443 kb on - strand, within BT1940 at 2442.478 kb on + strand, within BT1940 at 2442.479 kb on - strand, within BT1940 at 2442.480 kb on + strand, within BT1940 at 2442.480 kb on + strand, within BT1940 at 2442.480 kb on + strand, within BT1940 at 2442.481 kb on - strand, within BT1940 at 2442.481 kb on - strand, within BT1940 at 2442.481 kb on - strand, within BT1940 at 2442.563 kb on - strand, within BT1940 at 2442.683 kb on + strand, within BT1940 at 2442.736 kb on + strand, within BT1940 at 2442.737 kb on - strand, within BT1940 at 2442.796 kb on + strand, within BT1940 at 2442.829 kb on - strand, within BT1940 at 2442.860 kb on + strand, within BT1940 at 2442.860 kb on + strand, within BT1940 at 2442.860 kb on + strand, within BT1940 at 2442.861 kb on - strand, within BT1940 at 2442.861 kb on - strand, within BT1940 at 2442.862 kb on + strand, within BT1940 at 2442.910 kb on + strand, within BT1940 at 2442.911 kb on - strand, within BT1940 at 2442.920 kb on + strand, within BT1940 at 2442.920 kb on + strand, within BT1940 at 2442.921 kb on - strand, within BT1940 at 2442.921 kb on - strand, within BT1940 at 2442.988 kb on + strand, within BT1940 at 2442.988 kb on + strand, within BT1940 at 2442.989 kb on - strand, within BT1940 at 2443.051 kb on + strand at 2443.052 kb on - strand at 2443.083 kb on - strand at 2443.088 kb on + strand at 2443.088 kb on + strand at 2443.089 kb on - strand at 2443.108 kb on - strand at 2443.113 kb on + strand at 2443.114 kb on - strand at 2443.122 kb on + strand at 2443.471 kb on + strand at 2443.508 kb on - strand, within BT1941 at 2443.531 kb on + strand, within BT1941 at 2443.599 kb on - strand, within BT1941 at 2443.618 kb on + strand, within BT1941 at 2443.652 kb on - strand, within BT1941 at 2443.733 kb on + strand, within BT1941 at 2443.733 kb on + strand, within BT1941 at 2443.733 kb on + strand, within BT1941 at 2443.741 kb on + strand, within BT1941 at 2443.808 kb on + strand, within BT1941 at 2443.809 kb on - strand, within BT1941 at 2443.809 kb on - strand, within BT1941 at 2443.827 kb on - strand, within BT1941 at 2443.852 kb on + strand, within BT1941 at 2443.853 kb on - strand, within BT1941 at 2443.854 kb on + strand, within BT1941 at 2443.855 kb on - strand, within BT1941 at 2443.855 kb on - strand, within BT1941 at 2443.881 kb on - strand, within BT1941 at 2443.900 kb on - strand, within BT1941 at 2443.900 kb on - strand, within BT1941 at 2443.907 kb on + strand, within BT1941 at 2443.934 kb on - strand, within BT1941 at 2443.956 kb on + strand, within BT1941 at 2443.998 kb on - strand, within BT1941 at 2444.045 kb on - strand, within BT1941 at 2444.064 kb on - strand, within BT1941 at 2444.134 kb on - strand, within BT1941 at 2444.135 kb on + strand, within BT1941 at 2444.212 kb on + strand at 2444.255 kb on + strand at 2444.279 kb on - strand at 2444.315 kb on + strand at 2444.316 kb on - strand at 2444.316 kb on - strand at 2444.462 kb on - strand, within BT1942 at 2444.477 kb on - strand, within BT1942 at 2444.504 kb on + strand, within BT1942 at 2444.556 kb on + strand, within BT1942 at 2444.607 kb on - strand, within BT1942 at 2444.607 kb on - strand, within BT1942 at 2444.607 kb on - strand, within BT1942 at 2444.626 kb on + strand, within BT1942 at 2444.653 kb on + strand, within BT1942 at 2444.675 kb on - strand, within BT1942 at 2444.713 kb on - strand at 2444.762 kb on + strand at 2444.762 kb on + strand at 2444.832 kb on + strand at 2444.837 kb on + strand at 2444.956 kb on - strand at 2444.992 kb on - strand at 2445.009 kb on - strand at 2445.047 kb on - strand at 2445.080 kb on - strand at 2445.136 kb on + strand at 2445.137 kb on - strand at 2445.180 kb on + strand at 2445.188 kb on + strand at 2445.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with sodium fluoride 30 mM remove 2,442,425 + BT1940 0.55 -2.0 2,442,425 + BT1940 0.55 -1.5 2,442,426 - BT1940 0.55 -0.2 2,442,436 - BT1940 0.56 -3.2 2,442,443 - BT1940 0.56 +0.0 2,442,478 + BT1940 0.58 -0.1 2,442,479 - BT1940 0.58 -1.0 2,442,480 + BT1940 0.58 +1.0 2,442,480 + BT1940 0.58 +0.9 2,442,480 + BT1940 0.58 -1.0 2,442,481 - BT1940 0.58 +1.1 2,442,481 - BT1940 0.58 -1.2 2,442,481 - BT1940 0.58 -2.1 2,442,563 - BT1940 0.63 -0.2 2,442,683 + BT1940 0.71 -0.4 2,442,736 + BT1940 0.74 +1.4 2,442,737 - BT1940 0.74 +0.4 2,442,796 + BT1940 0.78 -1.2 2,442,829 - BT1940 0.80 +0.1 2,442,860 + BT1940 0.82 -1.3 2,442,860 + BT1940 0.82 -0.2 2,442,860 + BT1940 0.82 +0.4 2,442,861 - BT1940 0.82 +0.3 2,442,861 - BT1940 0.82 -0.2 2,442,862 + BT1940 0.82 -1.6 2,442,910 + BT1940 0.85 -2.0 2,442,911 - BT1940 0.85 -0.3 2,442,920 + BT1940 0.86 +0.2 2,442,920 + BT1940 0.86 -0.1 2,442,921 - BT1940 0.86 +1.4 2,442,921 - BT1940 0.86 +1.4 2,442,988 + BT1940 0.90 +0.2 2,442,988 + BT1940 0.90 -0.0 2,442,989 - BT1940 0.90 +0.4 2,443,051 + -2.6 2,443,052 - -0.2 2,443,083 - -0.1 2,443,088 + -0.6 2,443,088 + -1.3 2,443,089 - +0.8 2,443,108 - -0.3 2,443,113 + -0.5 2,443,114 - -1.9 2,443,122 + +0.5 2,443,471 + -0.1 2,443,508 - BT1941 0.11 -2.2 2,443,531 + BT1941 0.13 -1.5 2,443,599 - BT1941 0.22 -1.5 2,443,618 + BT1941 0.24 -1.6 2,443,652 - BT1941 0.28 -0.2 2,443,733 + BT1941 0.38 -3.3 2,443,733 + BT1941 0.38 -2.5 2,443,733 + BT1941 0.38 -0.3 2,443,741 + BT1941 0.39 -1.0 2,443,808 + BT1941 0.47 -1.1 2,443,809 - BT1941 0.47 -0.2 2,443,809 - BT1941 0.47 -0.1 2,443,827 - BT1941 0.49 -1.6 2,443,852 + BT1941 0.52 -3.6 2,443,853 - BT1941 0.52 -1.8 2,443,854 + BT1941 0.52 +0.5 2,443,855 - BT1941 0.52 -2.0 2,443,855 - BT1941 0.52 -0.6 2,443,881 - BT1941 0.55 -1.1 2,443,900 - BT1941 0.58 -2.8 2,443,900 - BT1941 0.58 -1.2 2,443,907 + BT1941 0.59 -1.2 2,443,934 - BT1941 0.62 +1.0 2,443,956 + BT1941 0.65 -0.7 2,443,998 - BT1941 0.70 +1.4 2,444,045 - BT1941 0.75 +0.8 2,444,064 - BT1941 0.77 -0.3 2,444,134 - BT1941 0.86 -1.3 2,444,135 + BT1941 0.86 -0.1 2,444,212 + -0.1 2,444,255 + +0.4 2,444,279 - -0.6 2,444,315 + -2.8 2,444,316 - -1.8 2,444,316 - +0.1 2,444,462 - BT1942 0.41 -1.0 2,444,477 - BT1942 0.44 -0.2 2,444,504 + BT1942 0.50 -1.8 2,444,556 + BT1942 0.62 +0.0 2,444,607 - BT1942 0.74 -0.2 2,444,607 - BT1942 0.74 +0.1 2,444,607 - BT1942 0.74 +0.5 2,444,626 + BT1942 0.78 -0.1 2,444,653 + BT1942 0.84 -0.0 2,444,675 - BT1942 0.89 -2.0 2,444,713 - -1.1 2,444,762 + +0.8 2,444,762 + +1.1 2,444,832 + -1.5 2,444,837 + -0.1 2,444,956 - -1.6 2,444,992 - +0.7 2,445,009 - -1.8 2,445,047 - -0.6 2,445,080 - -0.0 2,445,136 + -0.6 2,445,137 - +0.0 2,445,180 + -1.0 2,445,188 + -1.3 2,445,218 - -0.1
Or see this region's nucleotide sequence