Experiment: BHIS with sodium fluoride 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0102 and BT0103 are separated by 173 nucleotides BT0103 and BT0104 overlap by 4 nucleotides BT0104 and BT0105 overlap by 4 nucleotides
BT0102: BT0102 - conserved hypothetical protein (NCBI ptt file), at 92,986 to 94,995
BT0102
BT0103: BT0103 - conserved hypothetical protein (NCBI ptt file), at 95,169 to 96,704
BT0103
BT0104: BT0104 - hypothetical protein (NCBI ptt file), at 96,701 to 96,889
BT0104
BT0105: BT0105 - DNA topoisomerase I (Omega-protein) (NCBI ptt file), at 96,886 to 98,967
BT0105
Position (kb) 95
96
97 Strain fitness (log2 ratio) -2
-1
0
1
2 at 94.270 kb on - strand, within BT0102 at 94.315 kb on - strand, within BT0102 at 94.442 kb on + strand, within BT0102 at 94.635 kb on + strand, within BT0102 at 94.636 kb on - strand, within BT0102 at 94.816 kb on + strand at 94.976 kb on + strand at 95.057 kb on - strand at 95.074 kb on - strand at 95.111 kb on + strand at 95.112 kb on - strand at 95.231 kb on - strand at 95.406 kb on - strand, within BT0103 at 95.644 kb on - strand, within BT0103 at 95.644 kb on - strand, within BT0103 at 95.644 kb on - strand, within BT0103 at 95.644 kb on - strand, within BT0103 at 95.695 kb on + strand, within BT0103 at 95.748 kb on + strand, within BT0103 at 96.140 kb on - strand, within BT0103 at 96.332 kb on - strand, within BT0103 at 96.332 kb on - strand, within BT0103 at 96.337 kb on + strand, within BT0103 at 96.417 kb on + strand, within BT0103 at 96.418 kb on - strand, within BT0103 at 96.426 kb on + strand, within BT0103 at 96.429 kb on - strand, within BT0103 at 96.830 kb on + strand, within BT0104 at 96.883 kb on - strand at 97.075 kb on - strand at 97.161 kb on - strand, within BT0105 at 97.161 kb on - strand, within BT0105 at 97.199 kb on + strand, within BT0105 at 97.406 kb on + strand, within BT0105 at 97.422 kb on + strand, within BT0105 at 97.423 kb on - strand, within BT0105 at 97.499 kb on + strand, within BT0105 at 97.500 kb on - strand, within BT0105 at 97.500 kb on - strand, within BT0105 at 97.543 kb on + strand, within BT0105
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with sodium fluoride 30 mM remove 94,270 - BT0102 0.64 +1.6 94,315 - BT0102 0.66 -1.1 94,442 + BT0102 0.72 +1.3 94,635 + BT0102 0.82 -2.1 94,636 - BT0102 0.82 -0.9 94,816 + -0.7 94,976 + -2.0 95,057 - -0.6 95,074 - -1.0 95,111 + -2.1 95,112 - +0.6 95,231 - +1.3 95,406 - BT0103 0.15 +1.5 95,644 - BT0103 0.31 -2.1 95,644 - BT0103 0.31 -1.8 95,644 - BT0103 0.31 -0.7 95,644 - BT0103 0.31 +0.2 95,695 + BT0103 0.34 -1.8 95,748 + BT0103 0.38 -1.0 96,140 - BT0103 0.63 +1.9 96,332 - BT0103 0.76 -0.1 96,332 - BT0103 0.76 -0.0 96,337 + BT0103 0.76 +0.4 96,417 + BT0103 0.81 -1.5 96,418 - BT0103 0.81 +0.3 96,426 + BT0103 0.82 +0.5 96,429 - BT0103 0.82 +1.3 96,830 + BT0104 0.68 +1.2 96,883 - +0.1 97,075 - +1.3 97,161 - BT0105 0.13 -0.3 97,161 - BT0105 0.13 -2.0 97,199 + BT0105 0.15 -2.7 97,406 + BT0105 0.25 -2.4 97,422 + BT0105 0.26 +1.0 97,423 - BT0105 0.26 +0.3 97,499 + BT0105 0.29 +0.0 97,500 - BT0105 0.29 +0.0 97,500 - BT0105 0.29 +0.7 97,543 + BT0105 0.32 +1.9
Or see this region's nucleotide sequence