Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00177

Experiment: PYE with Gentamicin 0.0005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00174 and CCNA_00175 are separated by 11 nucleotidesCCNA_00175 and CCNA_00176 overlap by 86 nucleotidesCCNA_00176 and CCNA_00177 overlap by 32 nucleotidesCCNA_00177 and CCNA_00178 overlap by 4 nucleotidesCCNA_00178 and CCNA_00179 overlap by 4 nucleotides CCNA_00174: CCNA_00174 - type II secretion pathway protein F, at 189,809 to 191,020 _00174 CCNA_00175: CCNA_00175 - type II secretion pathway protein G, at 191,032 to 191,484 _00175 CCNA_00176: CCNA_00176 - type II secretion pathway protein H, at 191,399 to 191,956 _00176 CCNA_00177: CCNA_00177 - type II secretion pathway protein I, at 191,925 to 192,308 _00177 CCNA_00178: CCNA_00178 - type II secretion pathway protein J, at 192,305 to 192,895 _00178 CCNA_00179: CCNA_00179 - type II secretion pathway protein K, at 192,892 to 193,866 _00179 Position (kb) 191 192 193Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 191.009 kb on + strandat 191.022 kb on - strandat 191.037 kb on - strandat 191.081 kb on + strand, within CCNA_00175at 191.081 kb on + strand, within CCNA_00175at 191.089 kb on - strand, within CCNA_00175at 191.089 kb on - strand, within CCNA_00175at 191.096 kb on + strand, within CCNA_00175at 191.097 kb on + strand, within CCNA_00175at 191.099 kb on - strand, within CCNA_00175at 191.220 kb on - strand, within CCNA_00175at 191.229 kb on - strand, within CCNA_00175at 191.268 kb on + strand, within CCNA_00175at 191.300 kb on + strand, within CCNA_00175at 191.300 kb on + strand, within CCNA_00175at 191.307 kb on - strand, within CCNA_00175at 191.312 kb on - strand, within CCNA_00175at 191.312 kb on - strand, within CCNA_00175at 191.357 kb on - strand, within CCNA_00175at 191.357 kb on - strand, within CCNA_00175at 191.357 kb on - strand, within CCNA_00175at 191.357 kb on - strand, within CCNA_00175at 191.410 kb on - strandat 191.434 kb on - strandat 191.452 kb on + strandat 191.493 kb on - strand, within CCNA_00176at 191.493 kb on - strand, within CCNA_00176at 191.494 kb on + strand, within CCNA_00176at 191.502 kb on - strand, within CCNA_00176at 191.513 kb on + strand, within CCNA_00176at 191.518 kb on + strand, within CCNA_00176at 191.523 kb on + strand, within CCNA_00176at 191.523 kb on + strand, within CCNA_00176at 191.523 kb on + strand, within CCNA_00176at 191.531 kb on - strand, within CCNA_00176at 191.531 kb on - strand, within CCNA_00176at 191.531 kb on - strand, within CCNA_00176at 191.533 kb on + strand, within CCNA_00176at 191.539 kb on + strand, within CCNA_00176at 191.547 kb on - strand, within CCNA_00176at 191.572 kb on + strand, within CCNA_00176at 191.584 kb on + strand, within CCNA_00176at 191.585 kb on + strand, within CCNA_00176at 191.594 kb on - strand, within CCNA_00176at 191.594 kb on - strand, within CCNA_00176at 191.618 kb on + strand, within CCNA_00176at 191.672 kb on + strand, within CCNA_00176at 191.677 kb on + strand, within CCNA_00176at 191.703 kb on - strand, within CCNA_00176at 191.753 kb on + strand, within CCNA_00176at 191.761 kb on - strand, within CCNA_00176at 191.788 kb on + strand, within CCNA_00176at 191.796 kb on - strand, within CCNA_00176at 191.807 kb on - strand, within CCNA_00176at 191.843 kb on - strand, within CCNA_00176at 191.849 kb on + strand, within CCNA_00176at 191.857 kb on - strand, within CCNA_00176at 191.882 kb on - strand, within CCNA_00176at 191.919 kb on + strandat 191.962 kb on + strandat 192.018 kb on + strand, within CCNA_00177at 192.080 kb on + strand, within CCNA_00177at 192.138 kb on + strand, within CCNA_00177at 192.138 kb on + strand, within CCNA_00177at 192.146 kb on - strand, within CCNA_00177at 192.146 kb on - strand, within CCNA_00177at 192.146 kb on - strand, within CCNA_00177at 192.146 kb on - strand, within CCNA_00177at 192.184 kb on + strand, within CCNA_00177at 192.198 kb on + strand, within CCNA_00177at 192.251 kb on - strand, within CCNA_00177at 192.251 kb on - strand, within CCNA_00177at 192.290 kb on + strandat 192.290 kb on + strandat 192.290 kb on + strandat 192.290 kb on + strandat 192.290 kb on + strandat 192.290 kb on + strandat 192.298 kb on - strandat 192.298 kb on - strandat 192.298 kb on - strandat 192.310 kb on + strandat 192.312 kb on + strandat 192.320 kb on - strandat 192.341 kb on + strandat 192.349 kb on - strandat 192.366 kb on - strand, within CCNA_00178at 192.385 kb on - strand, within CCNA_00178at 192.395 kb on - strand, within CCNA_00178at 192.435 kb on + strand, within CCNA_00178at 192.486 kb on - strand, within CCNA_00178at 192.553 kb on - strand, within CCNA_00178at 192.579 kb on + strand, within CCNA_00178at 192.592 kb on - strand, within CCNA_00178at 192.622 kb on - strand, within CCNA_00178at 192.631 kb on + strandat 192.650 kb on - strand, within CCNA_00178at 192.652 kb on + strand, within CCNA_00178at 192.653 kb on + strand, within CCNA_00178at 192.653 kb on + strand, within CCNA_00178at 192.660 kb on - strand, within CCNA_00178at 192.661 kb on - strand, within CCNA_00178at 192.694 kb on - strand, within CCNA_00178at 192.696 kb on - strand, within CCNA_00178at 192.699 kb on - strand, within CCNA_00178at 192.699 kb on - strand, within CCNA_00178at 192.702 kb on + strand, within CCNA_00178at 192.729 kb on - strand, within CCNA_00178at 192.758 kb on - strand, within CCNA_00178at 192.769 kb on + strand, within CCNA_00178at 192.771 kb on - strand, within CCNA_00178at 192.776 kb on - strand, within CCNA_00178at 192.784 kb on - strand, within CCNA_00178at 192.818 kb on + strand, within CCNA_00178at 192.824 kb on + strand, within CCNA_00178at 192.832 kb on - strand, within CCNA_00178at 192.876 kb on + strandat 192.876 kb on + strandat 192.876 kb on - strandat 192.878 kb on - strandat 192.884 kb on - strandat 192.884 kb on - strandat 192.884 kb on - strandat 192.886 kb on + strandat 192.912 kb on - strandat 192.919 kb on + strandat 192.942 kb on - strandat 193.022 kb on + strand, within CCNA_00179at 193.041 kb on - strand, within CCNA_00179at 193.046 kb on - strand, within CCNA_00179at 193.052 kb on + strand, within CCNA_00179at 193.055 kb on - strand, within CCNA_00179at 193.112 kb on - strand, within CCNA_00179at 193.112 kb on - strand, within CCNA_00179at 193.113 kb on - strand, within CCNA_00179at 193.117 kb on - strand, within CCNA_00179at 193.123 kb on - strand, within CCNA_00179at 193.123 kb on - strand, within CCNA_00179at 193.164 kb on + strand, within CCNA_00179at 193.177 kb on - strand, within CCNA_00179at 193.183 kb on - strand, within CCNA_00179at 193.184 kb on - strand, within CCNA_00179at 193.191 kb on - strand, within CCNA_00179at 193.202 kb on + strand, within CCNA_00179at 193.215 kb on - strand, within CCNA_00179at 193.265 kb on + strand, within CCNA_00179at 193.275 kb on + strand, within CCNA_00179at 193.283 kb on - strand, within CCNA_00179at 193.290 kb on - strand, within CCNA_00179

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Gentamicin 0.0005 mg/ml
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191,009 + -0.6
191,022 - +0.6
191,037 - +0.3
191,081 + CCNA_00175 0.11 +1.1
191,081 + CCNA_00175 0.11 -0.7
191,089 - CCNA_00175 0.13 +0.7
191,089 - CCNA_00175 0.13 +1.1
191,096 + CCNA_00175 0.14 -1.0
191,097 + CCNA_00175 0.14 +0.3
191,099 - CCNA_00175 0.15 +0.2
191,220 - CCNA_00175 0.42 -0.5
191,229 - CCNA_00175 0.43 -0.1
191,268 + CCNA_00175 0.52 -0.9
191,300 + CCNA_00175 0.59 +0.1
191,300 + CCNA_00175 0.59 -0.9
191,307 - CCNA_00175 0.61 +0.4
191,312 - CCNA_00175 0.62 +0.9
191,312 - CCNA_00175 0.62 +0.0
191,357 - CCNA_00175 0.72 -0.4
191,357 - CCNA_00175 0.72 +2.0
191,357 - CCNA_00175 0.72 -2.0
191,357 - CCNA_00175 0.72 +0.1
191,410 - -0.2
191,434 - -0.1
191,452 + +0.7
191,493 - CCNA_00176 0.17 +0.3
191,493 - CCNA_00176 0.17 -0.7
191,494 + CCNA_00176 0.17 -1.4
191,502 - CCNA_00176 0.18 -1.2
191,513 + CCNA_00176 0.20 -1.4
191,518 + CCNA_00176 0.21 -0.2
191,523 + CCNA_00176 0.22 -1.1
191,523 + CCNA_00176 0.22 +1.9
191,523 + CCNA_00176 0.22 -0.9
191,531 - CCNA_00176 0.24 -1.6
191,531 - CCNA_00176 0.24 -0.5
191,531 - CCNA_00176 0.24 +0.4
191,533 + CCNA_00176 0.24 -2.4
191,539 + CCNA_00176 0.25 -0.0
191,547 - CCNA_00176 0.27 -0.8
191,572 + CCNA_00176 0.31 -2.3
191,584 + CCNA_00176 0.33 +0.4
191,585 + CCNA_00176 0.33 +0.4
191,594 - CCNA_00176 0.35 -0.1
191,594 - CCNA_00176 0.35 -0.8
191,618 + CCNA_00176 0.39 +0.1
191,672 + CCNA_00176 0.49 -0.6
191,677 + CCNA_00176 0.50 -1.7
191,703 - CCNA_00176 0.54 +0.3
191,753 + CCNA_00176 0.63 -0.8
191,761 - CCNA_00176 0.65 -1.3
191,788 + CCNA_00176 0.70 +1.2
191,796 - CCNA_00176 0.71 +0.6
191,807 - CCNA_00176 0.73 -1.4
191,843 - CCNA_00176 0.80 +0.7
191,849 + CCNA_00176 0.81 -1.2
191,857 - CCNA_00176 0.82 -1.4
191,882 - CCNA_00176 0.87 -0.1
191,919 + -0.6
191,962 + -3.3
192,018 + CCNA_00177 0.24 +0.1
192,080 + CCNA_00177 0.40 -0.6
192,138 + CCNA_00177 0.55 +0.0
192,138 + CCNA_00177 0.55 -1.1
192,146 - CCNA_00177 0.58 -1.0
192,146 - CCNA_00177 0.58 -1.8
192,146 - CCNA_00177 0.58 +0.3
192,146 - CCNA_00177 0.58 -0.5
192,184 + CCNA_00177 0.67 -2.3
192,198 + CCNA_00177 0.71 +0.2
192,251 - CCNA_00177 0.85 -0.1
192,251 - CCNA_00177 0.85 -1.1
192,290 + -0.7
192,290 + -1.8
192,290 + -0.1
192,290 + -1.1
192,290 + -1.2
192,290 + -1.3
192,298 - -1.0
192,298 - -2.2
192,298 - +0.6
192,310 + -0.8
192,312 + -0.5
192,320 - +0.9
192,341 + +0.6
192,349 - +0.1
192,366 - CCNA_00178 0.10 +0.1
192,385 - CCNA_00178 0.14 +0.4
192,395 - CCNA_00178 0.15 +0.4
192,435 + CCNA_00178 0.22 -2.1
192,486 - CCNA_00178 0.31 +0.1
192,553 - CCNA_00178 0.42 +0.3
192,579 + CCNA_00178 0.46 -0.7
192,592 - CCNA_00178 0.49 -1.6
192,622 - CCNA_00178 0.54 -0.2
192,631 + +0.7
192,650 - CCNA_00178 0.58 -1.2
192,652 + CCNA_00178 0.59 -1.3
192,653 + CCNA_00178 0.59 -0.4
192,653 + CCNA_00178 0.59 +0.7
192,660 - CCNA_00178 0.60 -0.5
192,661 - CCNA_00178 0.60 -1.8
192,694 - CCNA_00178 0.66 -0.9
192,696 - CCNA_00178 0.66 -0.7
192,699 - CCNA_00178 0.67 -0.6
192,699 - CCNA_00178 0.67 -1.0
192,702 + CCNA_00178 0.67 -1.1
192,729 - CCNA_00178 0.72 +0.1
192,758 - CCNA_00178 0.77 -0.4
192,769 + CCNA_00178 0.79 +0.8
192,771 - CCNA_00178 0.79 +0.4
192,776 - CCNA_00178 0.80 +0.3
192,784 - CCNA_00178 0.81 -0.5
192,818 + CCNA_00178 0.87 -0.8
192,824 + CCNA_00178 0.88 +1.5
192,832 - CCNA_00178 0.89 -1.5
192,876 + +0.3
192,876 + -1.9
192,876 - -0.6
192,878 - -0.9
192,884 - +0.1
192,884 - -0.4
192,884 - -0.7
192,886 + +1.1
192,912 - -0.8
192,919 + -0.5
192,942 - -0.4
193,022 + CCNA_00179 0.13 -0.4
193,041 - CCNA_00179 0.15 -0.1
193,046 - CCNA_00179 0.16 -1.0
193,052 + CCNA_00179 0.16 -0.4
193,055 - CCNA_00179 0.17 +0.9
193,112 - CCNA_00179 0.23 -0.3
193,112 - CCNA_00179 0.23 -1.5
193,113 - CCNA_00179 0.23 +0.0
193,117 - CCNA_00179 0.23 +1.0
193,123 - CCNA_00179 0.24 -1.5
193,123 - CCNA_00179 0.24 -2.0
193,164 + CCNA_00179 0.28 +0.9
193,177 - CCNA_00179 0.29 -0.8
193,183 - CCNA_00179 0.30 -0.6
193,184 - CCNA_00179 0.30 -0.4
193,191 - CCNA_00179 0.31 +0.1
193,202 + CCNA_00179 0.32 -1.9
193,215 - CCNA_00179 0.33 -1.1
193,265 + CCNA_00179 0.38 -0.9
193,275 + CCNA_00179 0.39 +0.1
193,283 - CCNA_00179 0.40 -2.1
193,290 - CCNA_00179 0.41 +0.6

Or see this region's nucleotide sequence