Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21960 and BPHYT_RS21965 are separated by 15 nucleotides BPHYT_RS21965 and BPHYT_RS21970 overlap by 4 nucleotides BPHYT_RS21970 and BPHYT_RS21975 are separated by 219 nucleotides
BPHYT_RS21960: BPHYT_RS21960 - nucleoside transporter, at 483,861 to 485,138
_RS21960
BPHYT_RS21965: BPHYT_RS21965 - cytidine deaminase, at 485,154 to 485,543
_RS21965
BPHYT_RS21970: BPHYT_RS21970 - nucleoside hydrolase, at 485,540 to 486,505
_RS21970
BPHYT_RS21975: BPHYT_RS21975 - 2-dehydropantoate 2-reductase, at 486,725 to 487,660
_RS21975
Position (kb) 485
486
487 Strain fitness (log2 ratio) -2
-1
0
1 at 484.593 kb on - strand, within BPHYT_RS21960 at 484.866 kb on + strand, within BPHYT_RS21960 at 485.015 kb on + strand at 485.535 kb on + strand at 485.536 kb on - strand at 485.540 kb on - strand at 485.822 kb on - strand, within BPHYT_RS21970 at 486.266 kb on + strand, within BPHYT_RS21970 at 486.267 kb on - strand, within BPHYT_RS21970 at 486.342 kb on + strand, within BPHYT_RS21970 at 486.636 kb on + strand at 486.736 kb on - strand at 486.738 kb on + strand at 487.046 kb on - strand, within BPHYT_RS21975 at 487.132 kb on + strand, within BPHYT_RS21975
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 484,593 - BPHYT_RS21960 0.57 -0.4 484,866 + BPHYT_RS21960 0.79 -0.2 485,015 + +0.2 485,535 + -1.3 485,536 - -1.7 485,540 - -1.6 485,822 - BPHYT_RS21970 0.29 -1.3 486,266 + BPHYT_RS21970 0.75 -2.3 486,267 - BPHYT_RS21970 0.75 -1.3 486,342 + BPHYT_RS21970 0.83 +1.2 486,636 + +0.0 486,736 - +0.6 486,738 + +0.4 487,046 - BPHYT_RS21975 0.34 -0.5 487,132 + BPHYT_RS21975 0.43 -0.3
Or see this region's nucleotide sequence