Experiment: casaminos (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS21570 and AZOBR_RS21575 overlap by 4 nucleotides AZOBR_RS21575 and AZOBR_RS21580 are separated by 15 nucleotides AZOBR_RS21580 and AZOBR_RS21585 are separated by 7 nucleotides AZOBR_RS21585 and AZOBR_RS21590 are separated by 13 nucleotides
AZOBR_RS21570: AZOBR_RS21570 - N-acetylneuraminate synthase, at 1,616,129 to 1,617,181
_RS21570
AZOBR_RS21575: AZOBR_RS21575 - spore coat protein, at 1,617,178 to 1,617,936
_RS21575
AZOBR_RS21580: AZOBR_RS21580 - hypothetical protein, at 1,617,952 to 1,618,185
_RS21580
AZOBR_RS21585: AZOBR_RS21585 - alpha/beta hydrolase, at 1,618,193 to 1,619,011
_RS21585
AZOBR_RS21590: AZOBR_RS21590 - hypothetical protein, at 1,619,025 to 1,619,303
_RS21590
Position (kb)
1617
1618
1619 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1617.002 kb on + strand, within AZOBR_RS21570 at 1617.003 kb on - strand, within AZOBR_RS21570 at 1617.696 kb on + strand, within AZOBR_RS21575 at 1617.697 kb on - strand, within AZOBR_RS21575 at 1617.700 kb on + strand, within AZOBR_RS21575 at 1618.260 kb on - strand at 1618.260 kb on - strand at 1618.514 kb on + strand, within AZOBR_RS21585 at 1618.514 kb on + strand, within AZOBR_RS21585 at 1618.514 kb on + strand, within AZOBR_RS21585 at 1618.515 kb on - strand at 1618.945 kb on + strand at 1618.946 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (N) remove 1,617,002 + AZOBR_RS21570 0.83 -0.9 1,617,003 - AZOBR_RS21570 0.83 -2.2 1,617,696 + AZOBR_RS21575 0.68 -3.2 1,617,697 - AZOBR_RS21575 0.68 -2.4 1,617,700 + AZOBR_RS21575 0.69 -1.6 1,618,260 - -0.8 1,618,260 - -1.0 1,618,514 + AZOBR_RS21585 0.39 -0.3 1,618,514 + AZOBR_RS21585 0.39 +0.1 1,618,514 + AZOBR_RS21585 0.39 +0.3 1,618,515 - -0.7 1,618,945 + +0.3 1,618,946 - -1.7
Or see this region's nucleotide sequence