Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4414 and Pf6N2E2_4415 are separated by 207 nucleotides Pf6N2E2_4415 and Pf6N2E2_4416 overlap by 4 nucleotides Pf6N2E2_4416 and Pf6N2E2_4417 are separated by 2 nucleotides Pf6N2E2_4417 and Pf6N2E2_4418 are separated by 13 nucleotides
Pf6N2E2_4414 - FIG00953808: hypothetical protein, at 5,148,368 to 5,148,691
_4414
Pf6N2E2_4415 - Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase, at 5,148,899 to 5,149,603
_4415
Pf6N2E2_4416 - Tyrosine recombinase XerC, at 5,149,600 to 5,150,499
_4416
Pf6N2E2_4417 - Protein of unknown function DUF484, at 5,150,502 to 5,151,224
_4417
Pf6N2E2_4418 - Diaminopimelate epimerase (EC 5.1.1.7), at 5,151,238 to 5,152,068
_4418
Position (kb)
5149
5150
5151 Strain fitness (log2 ratio)
-2
-1
0
1 at 5148.671 kb on + strand at 5148.671 kb on + strand at 5148.671 kb on + strand at 5148.671 kb on + strand at 5148.671 kb on + strand at 5148.685 kb on + strand at 5148.685 kb on + strand at 5148.686 kb on - strand at 5148.693 kb on + strand at 5148.837 kb on + strand at 5148.837 kb on + strand at 5148.837 kb on + strand at 5148.837 kb on + strand at 5148.838 kb on - strand at 5148.927 kb on - strand at 5149.180 kb on - strand, within Pf6N2E2_4415 at 5149.180 kb on - strand, within Pf6N2E2_4415 at 5149.523 kb on + strand, within Pf6N2E2_4415 at 5149.523 kb on + strand, within Pf6N2E2_4415 at 5149.523 kb on + strand, within Pf6N2E2_4415 at 5149.685 kb on + strand at 5149.720 kb on + strand, within Pf6N2E2_4416 at 5149.721 kb on - strand, within Pf6N2E2_4416 at 5150.040 kb on + strand, within Pf6N2E2_4416 at 5150.078 kb on + strand, within Pf6N2E2_4416 at 5150.079 kb on - strand, within Pf6N2E2_4416 at 5150.079 kb on - strand, within Pf6N2E2_4416 at 5150.253 kb on - strand, within Pf6N2E2_4416 at 5150.259 kb on - strand, within Pf6N2E2_4416 at 5150.509 kb on - strand at 5150.566 kb on + strand at 5150.566 kb on + strand at 5150.567 kb on - strand at 5150.599 kb on + strand, within Pf6N2E2_4417 at 5150.599 kb on + strand, within Pf6N2E2_4417 at 5150.600 kb on - strand, within Pf6N2E2_4417 at 5150.600 kb on - strand, within Pf6N2E2_4417 at 5150.600 kb on - strand, within Pf6N2E2_4417 at 5150.820 kb on - strand, within Pf6N2E2_4417 at 5150.979 kb on + strand, within Pf6N2E2_4417 at 5150.980 kb on - strand, within Pf6N2E2_4417 at 5150.980 kb on - strand, within Pf6N2E2_4417 at 5151.261 kb on - strand at 5151.262 kb on + strand at 5151.263 kb on - strand at 5151.263 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,148,671 + +0.7 5,148,671 + -0.2 5,148,671 + -0.0 5,148,671 + +0.1 5,148,671 + -1.1 5,148,685 + +0.3 5,148,685 + +0.7 5,148,686 - +0.5 5,148,693 + +1.3 5,148,837 + +0.7 5,148,837 + -0.2 5,148,837 + +0.8 5,148,837 + -0.0 5,148,838 - +0.6 5,148,927 - -0.3 5,149,180 - Pf6N2E2_4415 0.40 +0.4 5,149,180 - Pf6N2E2_4415 0.40 -1.3 5,149,523 + Pf6N2E2_4415 0.89 +0.3 5,149,523 + Pf6N2E2_4415 0.89 +0.5 5,149,523 + Pf6N2E2_4415 0.89 -0.3 5,149,685 + -0.2 5,149,720 + Pf6N2E2_4416 0.13 +0.2 5,149,721 - Pf6N2E2_4416 0.13 -2.1 5,150,040 + Pf6N2E2_4416 0.49 -2.4 5,150,078 + Pf6N2E2_4416 0.53 -0.5 5,150,079 - Pf6N2E2_4416 0.53 -1.8 5,150,079 - Pf6N2E2_4416 0.53 -2.1 5,150,253 - Pf6N2E2_4416 0.73 +0.2 5,150,259 - Pf6N2E2_4416 0.73 -2.2 5,150,509 - +0.6 5,150,566 + -1.0 5,150,566 + +0.1 5,150,567 - -1.3 5,150,599 + Pf6N2E2_4417 0.13 -1.4 5,150,599 + Pf6N2E2_4417 0.13 -2.7 5,150,600 - Pf6N2E2_4417 0.14 -1.2 5,150,600 - Pf6N2E2_4417 0.14 +0.7 5,150,600 - Pf6N2E2_4417 0.14 -0.5 5,150,820 - Pf6N2E2_4417 0.44 -1.4 5,150,979 + Pf6N2E2_4417 0.66 -0.1 5,150,980 - Pf6N2E2_4417 0.66 +0.0 5,150,980 - Pf6N2E2_4417 0.66 -0.2 5,151,261 - -1.2 5,151,262 + +0.7 5,151,263 - -0.2 5,151,263 - -0.2
Or see this region's nucleotide sequence