Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3746 and Pf6N2E2_3747 overlap by 4 nucleotides Pf6N2E2_3747 and Pf6N2E2_3748 overlap by 4 nucleotides Pf6N2E2_3748 and Pf6N2E2_3749 are separated by 67 nucleotides
Pf6N2E2_3746 - General secretion pathway protein D, at 4,360,838 to 4,362,772
_3746
Pf6N2E2_3747 - hypothetical protein, at 4,362,769 to 4,363,293
_3747
Pf6N2E2_3748 - Cytochrome B561, at 4,363,290 to 4,363,835
_3748
Pf6N2E2_3749 - Chitin binding protein, at 4,363,903 to 4,365,366
_3749
Position (kb)
4362
4363
4364 Strain fitness (log2 ratio)
-1
0
1 at 4362.292 kb on - strand, within Pf6N2E2_3746 at 4362.292 kb on - strand, within Pf6N2E2_3746 at 4362.292 kb on - strand, within Pf6N2E2_3746 at 4362.501 kb on + strand, within Pf6N2E2_3746 at 4362.501 kb on + strand, within Pf6N2E2_3746 at 4362.501 kb on + strand, within Pf6N2E2_3746 at 4362.501 kb on + strand, within Pf6N2E2_3746 at 4362.502 kb on - strand, within Pf6N2E2_3746 at 4362.502 kb on - strand, within Pf6N2E2_3746 at 4362.502 kb on - strand, within Pf6N2E2_3746 at 4362.735 kb on - strand at 4362.735 kb on - strand at 4362.796 kb on + strand at 4362.796 kb on + strand at 4362.800 kb on + strand at 4362.800 kb on + strand at 4362.800 kb on + strand at 4362.800 kb on + strand at 4362.801 kb on - strand at 4362.801 kb on - strand at 4362.977 kb on + strand, within Pf6N2E2_3747 at 4362.978 kb on - strand, within Pf6N2E2_3747 at 4363.053 kb on + strand, within Pf6N2E2_3747 at 4363.053 kb on + strand, within Pf6N2E2_3747 at 4363.053 kb on + strand, within Pf6N2E2_3747 at 4363.054 kb on - strand, within Pf6N2E2_3747 at 4363.054 kb on - strand, within Pf6N2E2_3747 at 4363.230 kb on - strand, within Pf6N2E2_3747 at 4363.347 kb on + strand, within Pf6N2E2_3748 at 4363.348 kb on - strand, within Pf6N2E2_3748 at 4363.433 kb on + strand, within Pf6N2E2_3748 at 4363.564 kb on + strand, within Pf6N2E2_3748 at 4363.564 kb on + strand, within Pf6N2E2_3748 at 4363.564 kb on + strand, within Pf6N2E2_3748 at 4363.565 kb on - strand, within Pf6N2E2_3748 at 4363.565 kb on - strand, within Pf6N2E2_3748 at 4363.750 kb on + strand, within Pf6N2E2_3748 at 4363.751 kb on - strand, within Pf6N2E2_3748 at 4363.751 kb on - strand, within Pf6N2E2_3748 at 4363.773 kb on + strand, within Pf6N2E2_3748 at 4363.773 kb on + strand, within Pf6N2E2_3748 at 4363.822 kb on + strand at 4363.823 kb on - strand at 4363.904 kb on + strand at 4363.904 kb on + strand at 4363.904 kb on + strand at 4363.904 kb on + strand at 4363.904 kb on + strand at 4363.904 kb on + strand at 4363.905 kb on - strand at 4363.905 kb on - strand at 4363.905 kb on - strand at 4363.973 kb on + strand at 4363.974 kb on - strand at 4364.060 kb on + strand, within Pf6N2E2_3749 at 4364.151 kb on + strand, within Pf6N2E2_3749 at 4364.151 kb on + strand, within Pf6N2E2_3749
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,362,292 - Pf6N2E2_3746 0.75 -0.4 4,362,292 - Pf6N2E2_3746 0.75 +0.5 4,362,292 - Pf6N2E2_3746 0.75 -0.4 4,362,501 + Pf6N2E2_3746 0.86 +0.6 4,362,501 + Pf6N2E2_3746 0.86 +0.8 4,362,501 + Pf6N2E2_3746 0.86 -0.4 4,362,501 + Pf6N2E2_3746 0.86 +0.2 4,362,502 - Pf6N2E2_3746 0.86 -0.2 4,362,502 - Pf6N2E2_3746 0.86 +0.3 4,362,502 - Pf6N2E2_3746 0.86 +0.5 4,362,735 - -0.2 4,362,735 - -0.8 4,362,796 + +0.1 4,362,796 + -0.8 4,362,800 + +0.5 4,362,800 + +0.2 4,362,800 + +0.5 4,362,800 + +0.6 4,362,801 - +1.3 4,362,801 - -0.1 4,362,977 + Pf6N2E2_3747 0.40 +0.0 4,362,978 - Pf6N2E2_3747 0.40 -0.9 4,363,053 + Pf6N2E2_3747 0.54 -1.7 4,363,053 + Pf6N2E2_3747 0.54 -0.4 4,363,053 + Pf6N2E2_3747 0.54 -0.5 4,363,054 - Pf6N2E2_3747 0.54 -0.2 4,363,054 - Pf6N2E2_3747 0.54 -0.6 4,363,230 - Pf6N2E2_3747 0.88 +0.2 4,363,347 + Pf6N2E2_3748 0.10 +0.3 4,363,348 - Pf6N2E2_3748 0.11 -0.2 4,363,433 + Pf6N2E2_3748 0.26 +0.9 4,363,564 + Pf6N2E2_3748 0.50 +0.4 4,363,564 + Pf6N2E2_3748 0.50 -1.0 4,363,564 + Pf6N2E2_3748 0.50 +0.3 4,363,565 - Pf6N2E2_3748 0.50 +0.6 4,363,565 - Pf6N2E2_3748 0.50 +0.2 4,363,750 + Pf6N2E2_3748 0.84 +0.3 4,363,751 - Pf6N2E2_3748 0.84 +0.2 4,363,751 - Pf6N2E2_3748 0.84 -0.2 4,363,773 + Pf6N2E2_3748 0.88 -0.6 4,363,773 + Pf6N2E2_3748 0.88 +0.3 4,363,822 + +0.3 4,363,823 - -0.2 4,363,904 + +0.7 4,363,904 + -0.1 4,363,904 + -0.6 4,363,904 + +0.2 4,363,904 + -1.6 4,363,904 + +0.1 4,363,905 - +0.5 4,363,905 - -1.3 4,363,905 - -1.2 4,363,973 + +0.4 4,363,974 - -0.0 4,364,060 + Pf6N2E2_3749 0.11 +0.3 4,364,151 + Pf6N2E2_3749 0.17 +0.7 4,364,151 + Pf6N2E2_3749 0.17 -0.9
Or see this region's nucleotide sequence