Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1313 and Pf6N2E2_1314 are separated by 14 nucleotides Pf6N2E2_1314 and Pf6N2E2_1315 are separated by 144 nucleotides Pf6N2E2_1315 and Pf6N2E2_1316 are separated by 252 nucleotides
Pf6N2E2_1313 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 1,547,280 to 1,548,083
_1313
Pf6N2E2_1314 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 1,548,098 to 1,548,895
_1314
Pf6N2E2_1315 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68), at 1,549,040 to 1,549,462
_1315
Pf6N2E2_1316 - P-hydroxyphenylacetate hydroxylase C1:reductase component, at 1,549,715 to 1,550,656
_1316
Position (kb)
1549
1550 Strain fitness (log2 ratio)
-1
0
1 at 1548.182 kb on + strand, within Pf6N2E2_1314 at 1548.450 kb on + strand, within Pf6N2E2_1314 at 1548.450 kb on + strand, within Pf6N2E2_1314 at 1548.450 kb on + strand, within Pf6N2E2_1314 at 1548.451 kb on - strand, within Pf6N2E2_1314 at 1548.451 kb on - strand, within Pf6N2E2_1314 at 1548.678 kb on + strand, within Pf6N2E2_1314 at 1548.770 kb on + strand, within Pf6N2E2_1314 at 1548.770 kb on + strand, within Pf6N2E2_1314 at 1548.770 kb on + strand, within Pf6N2E2_1314 at 1548.771 kb on - strand, within Pf6N2E2_1314 at 1548.811 kb on + strand, within Pf6N2E2_1314 at 1548.891 kb on - strand at 1549.104 kb on - strand, within Pf6N2E2_1315 at 1549.125 kb on + strand, within Pf6N2E2_1315 at 1549.497 kb on + strand at 1549.508 kb on + strand at 1549.702 kb on + strand at 1549.703 kb on - strand at 1549.859 kb on + strand, within Pf6N2E2_1316 at 1550.046 kb on + strand, within Pf6N2E2_1316 at 1550.046 kb on + strand, within Pf6N2E2_1316 at 1550.047 kb on - strand, within Pf6N2E2_1316 at 1550.047 kb on - strand, within Pf6N2E2_1316 at 1550.059 kb on - strand, within Pf6N2E2_1316 at 1550.059 kb on - strand, within Pf6N2E2_1316 at 1550.085 kb on + strand, within Pf6N2E2_1316 at 1550.085 kb on + strand, within Pf6N2E2_1316
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,548,182 + Pf6N2E2_1314 0.11 +0.6 1,548,450 + Pf6N2E2_1314 0.44 -0.3 1,548,450 + Pf6N2E2_1314 0.44 -0.4 1,548,450 + Pf6N2E2_1314 0.44 -0.7 1,548,451 - Pf6N2E2_1314 0.44 -0.4 1,548,451 - Pf6N2E2_1314 0.44 +0.2 1,548,678 + Pf6N2E2_1314 0.73 -1.0 1,548,770 + Pf6N2E2_1314 0.84 -0.1 1,548,770 + Pf6N2E2_1314 0.84 +0.0 1,548,770 + Pf6N2E2_1314 0.84 +1.2 1,548,771 - Pf6N2E2_1314 0.84 +1.0 1,548,811 + Pf6N2E2_1314 0.89 +0.8 1,548,891 - +0.4 1,549,104 - Pf6N2E2_1315 0.15 -1.6 1,549,125 + Pf6N2E2_1315 0.20 -0.1 1,549,497 + +0.4 1,549,508 + -0.9 1,549,702 + -0.2 1,549,703 - +0.2 1,549,859 + Pf6N2E2_1316 0.15 +0.3 1,550,046 + Pf6N2E2_1316 0.35 -0.6 1,550,046 + Pf6N2E2_1316 0.35 -1.1 1,550,047 - Pf6N2E2_1316 0.35 -0.0 1,550,047 - Pf6N2E2_1316 0.35 -1.0 1,550,059 - Pf6N2E2_1316 0.37 -0.6 1,550,059 - Pf6N2E2_1316 0.37 -0.2 1,550,085 + Pf6N2E2_1316 0.39 +0.9 1,550,085 + Pf6N2E2_1316 0.39 -0.2
Or see this region's nucleotide sequence