Experiment: MoYLS4 with Lomefloxacin 0.00156 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt valS and DvMF_2766 are separated by 383 nucleotides DvMF_2766 and DvMF_2767 are separated by 93 nucleotides DvMF_2767 and DvMF_2768 are separated by 269 nucleotides DvMF_2768 and DvMF_2769 are separated by 69 nucleotides
DvMF_2765: valS - valyl-tRNA synthetase (RefSeq), at 3,497,253 to 3,499,904
valS
DvMF_2766: DvMF_2766 - hypothetical protein (RefSeq), at 3,500,288 to 3,500,746
_2766
DvMF_2767: DvMF_2767 - uroporphyrin-III C-methyltransferase (RefSeq), at 3,500,840 to 3,502,351
_2767
DvMF_2768: DvMF_2768 - MOSC domain containing protein (RefSeq), at 3,502,621 to 3,503,055
_2768
DvMF_2769: DvMF_2769 - phosphoribosylglycinamide formyltransferase (RefSeq), at 3,503,125 to 3,503,808
_2769
Position (kb)
3500
3501
3502
3503 Strain fitness (log2 ratio)
-2
-1
0
1 at 3500.333 kb on + strand at 3500.426 kb on - strand, within DvMF_2766 at 3500.564 kb on + strand, within DvMF_2766 at 3500.683 kb on + strand, within DvMF_2766 at 3500.686 kb on - strand, within DvMF_2766 at 3502.471 kb on + strand at 3502.497 kb on + strand at 3502.623 kb on - strand at 3503.120 kb on + strand at 3503.327 kb on + strand, within DvMF_2769
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Lomefloxacin 0.00156 mM remove 3,500,333 + -0.1 3,500,426 - DvMF_2766 0.30 -1.9 3,500,564 + DvMF_2766 0.60 -0.6 3,500,683 + DvMF_2766 0.86 -1.3 3,500,686 - DvMF_2766 0.87 -1.0 3,502,471 + -1.7 3,502,497 + -0.4 3,502,623 - -1.6 3,503,120 + -0.7 3,503,327 + DvMF_2769 0.30 -0.5
Or see this region's nucleotide sequence