Experiment: MoYLS4 with Lomefloxacin 0.00156 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2617 and DvMF_2618 are separated by 299 nucleotides DvMF_2618 and DvMF_2619 are separated by 24 nucleotides DvMF_2619 and DvMF_2620 are separated by 158 nucleotides
DvMF_2617: DvMF_2617 - nitrogenase molybdenum-iron protein beta chain (RefSeq), at 3,305,945 to 3,307,321
_2617
DvMF_2618: DvMF_2618 - Dinitrogenase iron-molybdenum cofactor biosynthesis protein (RefSeq), at 3,307,621 to 3,308,880
_2618
DvMF_2619: DvMF_2619 - ferredoxin, 2Fe-2S (RefSeq), at 3,308,905 to 3,309,207
_2619
DvMF_2620: DvMF_2620 - nitrogenase MoFe cofactor biosynthesis protein NifE (RefSeq), at 3,309,366 to 3,310,790
_2620
Position (kb)
3307
3308
3309 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3306.634 kb on - strand, within DvMF_2617 at 3306.634 kb on - strand, within DvMF_2617 at 3306.668 kb on - strand, within DvMF_2617 at 3306.729 kb on + strand, within DvMF_2617 at 3306.739 kb on + strand, within DvMF_2617 at 3306.858 kb on - strand, within DvMF_2617 at 3307.114 kb on + strand, within DvMF_2617 at 3307.228 kb on - strand at 3307.230 kb on - strand at 3307.574 kb on - strand at 3307.585 kb on - strand at 3307.828 kb on + strand, within DvMF_2618 at 3307.836 kb on - strand, within DvMF_2618 at 3307.836 kb on - strand, within DvMF_2618 at 3308.016 kb on + strand, within DvMF_2618 at 3308.031 kb on + strand, within DvMF_2618 at 3308.153 kb on - strand, within DvMF_2618 at 3308.717 kb on - strand, within DvMF_2618 at 3308.848 kb on + strand at 3308.964 kb on - strand, within DvMF_2619 at 3309.124 kb on + strand, within DvMF_2619 at 3309.302 kb on - strand at 3309.504 kb on - strand at 3309.549 kb on + strand, within DvMF_2620 at 3309.551 kb on + strand, within DvMF_2620 at 3309.571 kb on - strand, within DvMF_2620 at 3309.585 kb on - strand, within DvMF_2620 at 3309.606 kb on + strand, within DvMF_2620
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Lomefloxacin 0.00156 mM remove 3,306,634 - DvMF_2617 0.50 -1.4 3,306,634 - DvMF_2617 0.50 -2.8 3,306,668 - DvMF_2617 0.53 -1.0 3,306,729 + DvMF_2617 0.57 -0.2 3,306,739 + DvMF_2617 0.58 -3.8 3,306,858 - DvMF_2617 0.66 -1.8 3,307,114 + DvMF_2617 0.85 -1.5 3,307,228 - -1.4 3,307,230 - -1.6 3,307,574 - +0.7 3,307,585 - -0.9 3,307,828 + DvMF_2618 0.16 -1.5 3,307,836 - DvMF_2618 0.17 -0.4 3,307,836 - DvMF_2618 0.17 -0.7 3,308,016 + DvMF_2618 0.31 -1.9 3,308,031 + DvMF_2618 0.33 -1.7 3,308,153 - DvMF_2618 0.42 -1.9 3,308,717 - DvMF_2618 0.87 -3.2 3,308,848 + -0.6 3,308,964 - DvMF_2619 0.19 +1.1 3,309,124 + DvMF_2619 0.72 -2.1 3,309,302 - -1.0 3,309,504 - -1.1 3,309,549 + DvMF_2620 0.13 -0.3 3,309,551 + DvMF_2620 0.13 -0.8 3,309,571 - DvMF_2620 0.14 -2.0 3,309,585 - DvMF_2620 0.15 -2.3 3,309,606 + DvMF_2620 0.17 -2.7
Or see this region's nucleotide sequence