Experiment: MoYLS4 with Lomefloxacin 0.00156 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1171 and DvMF_1172 are separated by 124 nucleotides DvMF_1172 and DvMF_1173 are separated by 148 nucleotides DvMF_1173 and DvMF_1174 are separated by 340 nucleotides
DvMF_1171: DvMF_1171 - C_GCAxxG_C_C family protein (RefSeq), at 1,448,325 to 1,449,098
_1171
DvMF_1172: DvMF_1172 - Rhodanese domain protein (RefSeq), at 1,449,223 to 1,449,744
_1172
DvMF_1173: DvMF_1173 - hypothetical protein (RefSeq), at 1,449,893 to 1,450,369
_1173
DvMF_1174: DvMF_1174 - cobalamin (vitamin B12) biosynthesis CbiD protein (RefSeq), at 1,450,710 to 1,452,059
_1174
Position (kb)
1449
1450
1451 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1448.919 kb on - strand, within DvMF_1171 at 1448.971 kb on - strand, within DvMF_1171 at 1449.000 kb on + strand, within DvMF_1171 at 1449.244 kb on + strand at 1449.244 kb on + strand at 1449.252 kb on - strand at 1449.252 kb on - strand at 1449.299 kb on - strand, within DvMF_1172 at 1449.346 kb on - strand, within DvMF_1172 at 1449.649 kb on - strand, within DvMF_1172 at 1449.687 kb on + strand, within DvMF_1172 at 1449.695 kb on - strand at 1449.952 kb on + strand, within DvMF_1173 at 1449.960 kb on - strand, within DvMF_1173 at 1450.004 kb on + strand, within DvMF_1173 at 1450.200 kb on - strand, within DvMF_1173 at 1450.872 kb on - strand, within DvMF_1174 at 1451.043 kb on - strand, within DvMF_1174 at 1451.337 kb on - strand, within DvMF_1174
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Lomefloxacin 0.00156 mM remove 1,448,919 - DvMF_1171 0.77 -2.7 1,448,971 - DvMF_1171 0.83 -4.1 1,449,000 + DvMF_1171 0.87 +0.7 1,449,244 + -1.6 1,449,244 + -4.1 1,449,252 - -4.2 1,449,252 - +0.2 1,449,299 - DvMF_1172 0.15 -1.0 1,449,346 - DvMF_1172 0.24 -1.5 1,449,649 - DvMF_1172 0.82 -0.9 1,449,687 + DvMF_1172 0.89 -0.9 1,449,695 - -0.8 1,449,952 + DvMF_1173 0.12 -1.6 1,449,960 - DvMF_1173 0.14 -1.8 1,450,004 + DvMF_1173 0.23 -1.4 1,450,200 - DvMF_1173 0.64 -2.4 1,450,872 - DvMF_1174 0.12 +0.3 1,451,043 - DvMF_1174 0.25 +0.6 1,451,337 - DvMF_1174 0.46 -2.3
Or see this region's nucleotide sequence