Experiment: MoYLS4 with Lomefloxacin 0.00156 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpsU and DvMF_0499 are separated by 195 nucleotides DvMF_0499 and DvMF_0500 are separated by 15 nucleotides DvMF_0500 and ksgA are separated by 191 nucleotides ksgA and DvMF_0502 are separated by 197 nucleotides DvMF_0502 and DvMF_0503 are separated by 57 nucleotides
DvMF_0498: rpsU - 30S ribosomal protein S21 (RefSeq), at 586,768 to 586,971
rpsU
DvMF_0499: DvMF_0499 - hypothetical protein (RefSeq), at 587,167 to 587,355
_0499
DvMF_0500: DvMF_0500 - histone family protein DNA-binding protein (RefSeq), at 587,371 to 587,643
_0500
DvMF_0501: ksgA - dimethyladenosine transferase (RefSeq), at 587,835 to 588,647
ksgA
DvMF_0502: DvMF_0502 - hypothetical protein (RefSeq), at 588,845 to 589,477
_0502
DvMF_0503: DvMF_0503 - elongation factor G (RefSeq), at 589,535 to 591,631
_0503
Position (kb) 587
588
589 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 587.057 kb on - strand at 587.178 kb on - strand at 587.259 kb on + strand, within DvMF_0499 at 587.482 kb on + strand, within DvMF_0500 at 587.514 kb on + strand, within DvMF_0500 at 587.522 kb on - strand, within DvMF_0500 at 587.590 kb on - strand, within DvMF_0500 at 587.633 kb on + strand at 587.792 kb on - strand at 587.792 kb on - strand at 588.137 kb on + strand, within ksgA at 588.792 kb on + strand at 589.392 kb on - strand, within DvMF_0502 at 589.458 kb on + strand at 589.491 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Lomefloxacin 0.00156 mM remove 587,057 - -1.2 587,178 - -2.2 587,259 + DvMF_0499 0.49 -1.0 587,482 + DvMF_0500 0.41 +0.4 587,514 + DvMF_0500 0.52 -1.3 587,522 - DvMF_0500 0.55 -4.4 587,590 - DvMF_0500 0.80 -0.0 587,633 + +0.7 587,792 - -3.4 587,792 - -0.2 588,137 + ksgA DvMF_0501 0.37 -2.2 588,792 + -0.8 589,392 - DvMF_0502 0.86 -3.1 589,458 + -0.9 589,491 + -0.8
Or see this region's nucleotide sequence