Experiment: MoYLS4 with Lomefloxacin 0.00156 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0117 and DvMF_0118 overlap by 1 nucleotides DvMF_0118 and DvMF_0119 are separated by 6 nucleotides DvMF_0119 and ispG are separated by 428 nucleotides
DvMF_0117: DvMF_0117 - ABC-3 protein (RefSeq), at 127,653 to 128,483
_0117
DvMF_0118: DvMF_0118 - ABC transporter related (RefSeq), at 128,483 to 129,409
_0118
DvMF_0119: DvMF_0119 - periplasmic solute binding protein (RefSeq), at 129,416 to 130,450
_0119
DvMF_0120: ispG - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (RefSeq), at 130,879 to 131,970
ispG
Position (kb) 129
130
131 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 128.702 kb on + strand, within DvMF_0118 at 128.712 kb on - strand, within DvMF_0118 at 128.734 kb on - strand, within DvMF_0118 at 128.784 kb on - strand, within DvMF_0118 at 128.784 kb on - strand, within DvMF_0118 at 128.802 kb on + strand, within DvMF_0118 at 129.442 kb on - strand at 129.493 kb on - strand at 129.582 kb on + strand, within DvMF_0119 at 129.676 kb on + strand, within DvMF_0119 at 129.684 kb on - strand, within DvMF_0119 at 129.767 kb on + strand, within DvMF_0119 at 129.767 kb on + strand, within DvMF_0119 at 129.843 kb on + strand, within DvMF_0119 at 130.103 kb on - strand, within DvMF_0119 at 130.431 kb on + strand at 130.432 kb on + strand at 130.566 kb on + strand at 130.717 kb on + strand at 130.731 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Lomefloxacin 0.00156 mM remove 128,702 + DvMF_0118 0.24 -0.2 128,712 - DvMF_0118 0.25 -2.5 128,734 - DvMF_0118 0.27 -0.1 128,784 - DvMF_0118 0.32 -0.3 128,784 - DvMF_0118 0.32 -1.6 128,802 + DvMF_0118 0.34 -1.8 129,442 - -3.5 129,493 - +0.1 129,582 + DvMF_0119 0.16 -0.6 129,676 + DvMF_0119 0.25 -1.9 129,684 - DvMF_0119 0.26 -1.6 129,767 + DvMF_0119 0.34 -1.9 129,767 + DvMF_0119 0.34 -1.0 129,843 + DvMF_0119 0.41 -1.4 130,103 - DvMF_0119 0.66 -1.5 130,431 + -2.3 130,432 + -2.8 130,566 + -0.8 130,717 + -1.7 130,731 - -0.8
Or see this region's nucleotide sequence