Experiment: MoYLS4 with Lomefloxacin 0.00156 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0027 and DvMF_0028 are separated by 137 nucleotides DvMF_0028 and DvMF_0029 are separated by 202 nucleotides DvMF_0029 and DvMF_0030 are separated by 756 nucleotides
DvMF_0027: DvMF_0027 - indolepyruvate/phenylpyruvate decarboxylase (RefSeq), at 34,593 to 36,311
_0027
DvMF_0028: DvMF_0028 - hypothetical protein (RefSeq), at 36,449 to 37,012
_0028
DvMF_0029: DvMF_0029 - hypothetical protein (RefSeq), at 37,215 to 37,841
_0029
DvMF_0030: DvMF_0030 - hypothetical protein (RefSeq), at 38,598 to 38,939
_0030
Position (kb) 37
38 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 36.346 kb on + strand at 36.902 kb on - strand, within DvMF_0028 at 37.041 kb on - strand at 37.177 kb on + strand at 37.487 kb on + strand, within DvMF_0029 at 37.843 kb on + strand at 37.977 kb on - strand at 37.997 kb on + strand at 38.069 kb on + strand at 38.140 kb on - strand at 38.251 kb on + strand at 38.371 kb on + strand at 38.400 kb on + strand at 38.469 kb on - strand at 38.482 kb on - strand at 38.558 kb on - strand at 38.569 kb on - strand at 38.663 kb on + strand, within DvMF_0030 at 38.703 kb on + strand, within DvMF_0030 at 38.788 kb on - strand, within DvMF_0030
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Lomefloxacin 0.00156 mM remove 36,346 + -1.0 36,902 - DvMF_0028 0.80 -0.8 37,041 - +0.4 37,177 + -2.7 37,487 + DvMF_0029 0.43 +0.1 37,843 + +2.2 37,977 - -2.6 37,997 + -0.3 38,069 + -0.7 38,140 - -0.5 38,251 + -1.8 38,371 + -2.1 38,400 + -3.4 38,469 - -0.5 38,482 - -1.2 38,558 - -1.2 38,569 - -0.2 38,663 + DvMF_0030 0.19 -0.4 38,703 + DvMF_0030 0.31 -2.5 38,788 - DvMF_0030 0.56 -0.2
Or see this region's nucleotide sequence