Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1916 and Pf6N2E2_1917 overlap by 11 nucleotides Pf6N2E2_1917 and Pf6N2E2_1918 are separated by 498 nucleotides Pf6N2E2_1918 and Pf6N2E2_1919 are separated by 205 nucleotides
Pf6N2E2_1916 - Transcriptional regulator, LysR family, at 2,270,692 to 2,270,970
_1916
Pf6N2E2_1917 - hypothetical protein, at 2,270,960 to 2,271,241
_1917
Pf6N2E2_1918 - Major facilitator family transporter, at 2,271,740 to 2,272,900
_1918
Pf6N2E2_1919 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,273,106 to 2,274,578
_1919
Position (kb)
2271
2272
2273 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2270.839 kb on - strand, within Pf6N2E2_1916 at 2270.839 kb on - strand, within Pf6N2E2_1916 at 2270.839 kb on - strand, within Pf6N2E2_1916 at 2270.860 kb on + strand, within Pf6N2E2_1916 at 2270.861 kb on - strand, within Pf6N2E2_1916 at 2270.861 kb on - strand, within Pf6N2E2_1916 at 2271.155 kb on - strand, within Pf6N2E2_1917 at 2271.156 kb on + strand, within Pf6N2E2_1917 at 2271.157 kb on - strand, within Pf6N2E2_1917 at 2271.157 kb on - strand, within Pf6N2E2_1917 at 2271.256 kb on + strand at 2271.257 kb on - strand at 2271.257 kb on - strand at 2271.272 kb on - strand at 2271.398 kb on + strand at 2271.425 kb on - strand at 2271.441 kb on - strand at 2271.571 kb on - strand at 2271.725 kb on - strand at 2271.725 kb on - strand at 2271.725 kb on - strand at 2271.727 kb on - strand at 2271.727 kb on - strand at 2271.737 kb on - strand at 2271.828 kb on - strand at 2271.828 kb on - strand at 2271.828 kb on - strand at 2271.828 kb on - strand at 2271.828 kb on - strand at 2271.828 kb on - strand at 2271.840 kb on + strand at 2271.841 kb on - strand at 2271.841 kb on - strand at 2271.846 kb on - strand at 2271.928 kb on + strand, within Pf6N2E2_1918 at 2271.968 kb on - strand, within Pf6N2E2_1918 at 2272.102 kb on + strand, within Pf6N2E2_1918 at 2272.102 kb on + strand, within Pf6N2E2_1918 at 2272.102 kb on + strand, within Pf6N2E2_1918 at 2272.103 kb on - strand, within Pf6N2E2_1918 at 2272.128 kb on - strand, within Pf6N2E2_1918 at 2272.216 kb on - strand, within Pf6N2E2_1918 at 2272.216 kb on - strand, within Pf6N2E2_1918 at 2272.216 kb on - strand, within Pf6N2E2_1918 at 2272.221 kb on + strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.231 kb on - strand, within Pf6N2E2_1918 at 2272.398 kb on + strand, within Pf6N2E2_1918 at 2272.436 kb on - strand at 2272.605 kb on + strand, within Pf6N2E2_1918 at 2272.628 kb on - strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.737 kb on + strand, within Pf6N2E2_1918 at 2272.737 kb on + strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.847 kb on - strand at 2272.973 kb on + strand at 2272.973 kb on + strand at 2273.018 kb on + strand at 2273.019 kb on - strand at 2273.127 kb on + strand at 2273.149 kb on + strand at 2273.149 kb on + strand at 2273.149 kb on + strand at 2273.150 kb on - strand at 2273.334 kb on + strand, within Pf6N2E2_1919 at 2273.431 kb on - strand, within Pf6N2E2_1919 at 2273.431 kb on - strand, within Pf6N2E2_1919 at 2273.431 kb on - strand, within Pf6N2E2_1919 at 2273.482 kb on - strand, within Pf6N2E2_1919 at 2273.482 kb on - strand, within Pf6N2E2_1919 at 2273.617 kb on - strand, within Pf6N2E2_1919 at 2273.659 kb on + strand, within Pf6N2E2_1919 at 2273.660 kb on - strand, within Pf6N2E2_1919 at 2273.664 kb on - strand, within Pf6N2E2_1919 at 2273.664 kb on - strand, within Pf6N2E2_1919
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 2,270,839 - Pf6N2E2_1916 0.53 -0.4 2,270,839 - Pf6N2E2_1916 0.53 +0.2 2,270,839 - Pf6N2E2_1916 0.53 -1.0 2,270,860 + Pf6N2E2_1916 0.60 +1.0 2,270,861 - Pf6N2E2_1916 0.61 -0.1 2,270,861 - Pf6N2E2_1916 0.61 +0.2 2,271,155 - Pf6N2E2_1917 0.69 +0.6 2,271,156 + Pf6N2E2_1917 0.70 +0.9 2,271,157 - Pf6N2E2_1917 0.70 -1.1 2,271,157 - Pf6N2E2_1917 0.70 +0.3 2,271,256 + +0.8 2,271,257 - +0.6 2,271,257 - +0.4 2,271,272 - +0.7 2,271,398 + +0.5 2,271,425 - +0.1 2,271,441 - -0.8 2,271,571 - -0.4 2,271,725 - -0.1 2,271,725 - +0.0 2,271,725 - +0.2 2,271,727 - +0.1 2,271,727 - +0.3 2,271,737 - -0.9 2,271,828 - -0.3 2,271,828 - +0.1 2,271,828 - +0.8 2,271,828 - +0.3 2,271,828 - -0.4 2,271,828 - +0.3 2,271,840 + +0.1 2,271,841 - -0.1 2,271,841 - -2.3 2,271,846 - +0.4 2,271,928 + Pf6N2E2_1918 0.16 -0.2 2,271,968 - Pf6N2E2_1918 0.20 -0.3 2,272,102 + Pf6N2E2_1918 0.31 +0.3 2,272,102 + Pf6N2E2_1918 0.31 +1.0 2,272,102 + Pf6N2E2_1918 0.31 -0.6 2,272,103 - Pf6N2E2_1918 0.31 +0.4 2,272,128 - Pf6N2E2_1918 0.33 +0.7 2,272,216 - Pf6N2E2_1918 0.41 -0.1 2,272,216 - Pf6N2E2_1918 0.41 -0.0 2,272,216 - Pf6N2E2_1918 0.41 -0.1 2,272,221 + Pf6N2E2_1918 0.41 -0.1 2,272,222 - Pf6N2E2_1918 0.42 -0.4 2,272,222 - Pf6N2E2_1918 0.42 +0.4 2,272,222 - Pf6N2E2_1918 0.42 +0.0 2,272,222 - Pf6N2E2_1918 0.42 -0.8 2,272,231 - Pf6N2E2_1918 0.42 -0.1 2,272,398 + Pf6N2E2_1918 0.57 +0.8 2,272,436 - +1.9 2,272,605 + Pf6N2E2_1918 0.75 +0.4 2,272,628 - Pf6N2E2_1918 0.76 +0.4 2,272,665 + Pf6N2E2_1918 0.80 +0.5 2,272,665 + Pf6N2E2_1918 0.80 +0.3 2,272,665 + Pf6N2E2_1918 0.80 -0.2 2,272,665 + Pf6N2E2_1918 0.80 +0.2 2,272,665 + Pf6N2E2_1918 0.80 +0.0 2,272,666 - Pf6N2E2_1918 0.80 +0.7 2,272,666 - Pf6N2E2_1918 0.80 -0.1 2,272,666 - Pf6N2E2_1918 0.80 +0.6 2,272,666 - Pf6N2E2_1918 0.80 +0.3 2,272,737 + Pf6N2E2_1918 0.86 -0.3 2,272,737 + Pf6N2E2_1918 0.86 -0.4 2,272,738 - Pf6N2E2_1918 0.86 +0.0 2,272,738 - Pf6N2E2_1918 0.86 +0.2 2,272,738 - Pf6N2E2_1918 0.86 -1.2 2,272,738 - Pf6N2E2_1918 0.86 -0.7 2,272,738 - Pf6N2E2_1918 0.86 +0.7 2,272,738 - Pf6N2E2_1918 0.86 +0.5 2,272,738 - Pf6N2E2_1918 0.86 -1.2 2,272,847 - -1.2 2,272,973 + -0.9 2,272,973 + +0.2 2,273,018 + +0.6 2,273,019 - -0.0 2,273,127 + -0.1 2,273,149 + +1.1 2,273,149 + +0.7 2,273,149 + -1.1 2,273,150 - -0.0 2,273,334 + Pf6N2E2_1919 0.15 -0.7 2,273,431 - Pf6N2E2_1919 0.22 -0.4 2,273,431 - Pf6N2E2_1919 0.22 -0.3 2,273,431 - Pf6N2E2_1919 0.22 +0.4 2,273,482 - Pf6N2E2_1919 0.26 -0.4 2,273,482 - Pf6N2E2_1919 0.26 +1.0 2,273,617 - Pf6N2E2_1919 0.35 -0.1 2,273,659 + Pf6N2E2_1919 0.38 +0.1 2,273,660 - Pf6N2E2_1919 0.38 -0.8 2,273,664 - Pf6N2E2_1919 0.38 +0.5 2,273,664 - Pf6N2E2_1919 0.38 +0.9
Or see this region's nucleotide sequence