Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1855 and Pf6N2E2_1856 are separated by 26 nucleotides Pf6N2E2_1856 and Pf6N2E2_1857 are separated by 224 nucleotides Pf6N2E2_1857 and Pf6N2E2_1858 overlap by 4 nucleotides
Pf6N2E2_1855 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 2,199,703 to 2,200,854
_1855
Pf6N2E2_1856 - phytoene dehydrogenase family protein, at 2,200,881 to 2,202,287
_1856
Pf6N2E2_1857 - hypothetical protein, at 2,202,512 to 2,202,991
_1857
Pf6N2E2_1858 - Cytochrome c oxidase polypeptide III (EC 1.9.3.1), at 2,202,988 to 2,203,623
_1858
Position (kb)
2200
2201
2202
2203 Strain fitness (log2 ratio)
-1
0
1
2 at 2199.961 kb on - strand, within Pf6N2E2_1855 at 2200.006 kb on + strand, within Pf6N2E2_1855 at 2200.007 kb on - strand, within Pf6N2E2_1855 at 2200.159 kb on + strand, within Pf6N2E2_1855 at 2200.160 kb on - strand, within Pf6N2E2_1855 at 2200.160 kb on - strand, within Pf6N2E2_1855 at 2200.160 kb on - strand, within Pf6N2E2_1855 at 2200.160 kb on - strand, within Pf6N2E2_1855 at 2200.160 kb on - strand, within Pf6N2E2_1855 at 2200.160 kb on - strand, within Pf6N2E2_1855 at 2200.418 kb on - strand, within Pf6N2E2_1855 at 2200.511 kb on - strand, within Pf6N2E2_1855 at 2200.511 kb on - strand, within Pf6N2E2_1855 at 2200.553 kb on + strand, within Pf6N2E2_1855 at 2200.553 kb on + strand, within Pf6N2E2_1855 at 2200.554 kb on - strand, within Pf6N2E2_1855 at 2200.637 kb on - strand, within Pf6N2E2_1855 at 2200.640 kb on + strand, within Pf6N2E2_1855 at 2200.640 kb on + strand, within Pf6N2E2_1855 at 2200.640 kb on + strand, within Pf6N2E2_1855 at 2200.640 kb on + strand, within Pf6N2E2_1855 at 2200.640 kb on + strand, within Pf6N2E2_1855 at 2200.641 kb on - strand, within Pf6N2E2_1855 at 2200.641 kb on - strand, within Pf6N2E2_1855 at 2200.641 kb on - strand, within Pf6N2E2_1855 at 2200.747 kb on + strand at 2200.748 kb on - strand at 2200.748 kb on - strand at 2200.749 kb on + strand at 2200.749 kb on + strand at 2200.749 kb on + strand at 2200.750 kb on - strand at 2200.750 kb on - strand at 2200.750 kb on - strand at 2200.777 kb on + strand at 2200.777 kb on + strand at 2200.778 kb on - strand at 2200.804 kb on + strand at 2200.805 kb on - strand at 2200.895 kb on - strand at 2200.910 kb on - strand at 2201.071 kb on + strand, within Pf6N2E2_1856 at 2201.072 kb on - strand, within Pf6N2E2_1856 at 2201.072 kb on - strand, within Pf6N2E2_1856 at 2201.076 kb on - strand, within Pf6N2E2_1856 at 2201.144 kb on - strand, within Pf6N2E2_1856 at 2201.168 kb on + strand, within Pf6N2E2_1856 at 2201.170 kb on + strand, within Pf6N2E2_1856 at 2201.170 kb on + strand, within Pf6N2E2_1856 at 2201.170 kb on + strand, within Pf6N2E2_1856 at 2201.170 kb on + strand, within Pf6N2E2_1856 at 2201.171 kb on - strand, within Pf6N2E2_1856 at 2201.171 kb on - strand, within Pf6N2E2_1856 at 2201.171 kb on - strand, within Pf6N2E2_1856 at 2201.171 kb on - strand, within Pf6N2E2_1856 at 2201.171 kb on - strand, within Pf6N2E2_1856 at 2201.213 kb on + strand, within Pf6N2E2_1856 at 2201.213 kb on + strand, within Pf6N2E2_1856 at 2201.216 kb on - strand, within Pf6N2E2_1856 at 2201.222 kb on - strand, within Pf6N2E2_1856 at 2201.327 kb on - strand, within Pf6N2E2_1856 at 2201.398 kb on - strand, within Pf6N2E2_1856 at 2201.624 kb on - strand, within Pf6N2E2_1856 at 2201.676 kb on + strand, within Pf6N2E2_1856 at 2201.677 kb on - strand, within Pf6N2E2_1856 at 2201.800 kb on - strand, within Pf6N2E2_1856 at 2201.914 kb on + strand, within Pf6N2E2_1856 at 2201.914 kb on + strand, within Pf6N2E2_1856 at 2201.915 kb on - strand, within Pf6N2E2_1856 at 2202.160 kb on - strand at 2202.283 kb on + strand at 2202.370 kb on - strand at 2202.405 kb on + strand at 2202.593 kb on - strand, within Pf6N2E2_1857 at 2202.593 kb on - strand, within Pf6N2E2_1857 at 2202.593 kb on - strand, within Pf6N2E2_1857 at 2202.593 kb on - strand, within Pf6N2E2_1857 at 2202.705 kb on + strand, within Pf6N2E2_1857 at 2202.705 kb on + strand, within Pf6N2E2_1857 at 2202.705 kb on + strand, within Pf6N2E2_1857 at 2202.706 kb on - strand, within Pf6N2E2_1857 at 2202.816 kb on + strand, within Pf6N2E2_1857 at 2202.826 kb on - strand, within Pf6N2E2_1857 at 2202.833 kb on + strand, within Pf6N2E2_1857 at 2202.916 kb on - strand, within Pf6N2E2_1857 at 2203.236 kb on - strand, within Pf6N2E2_1858 at 2203.236 kb on - strand, within Pf6N2E2_1858
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 2,199,961 - Pf6N2E2_1855 0.22 -0.5 2,200,006 + Pf6N2E2_1855 0.26 +0.3 2,200,007 - Pf6N2E2_1855 0.26 -0.5 2,200,159 + Pf6N2E2_1855 0.40 -1.5 2,200,160 - Pf6N2E2_1855 0.40 -0.4 2,200,160 - Pf6N2E2_1855 0.40 +0.6 2,200,160 - Pf6N2E2_1855 0.40 -0.0 2,200,160 - Pf6N2E2_1855 0.40 -0.2 2,200,160 - Pf6N2E2_1855 0.40 +0.5 2,200,160 - Pf6N2E2_1855 0.40 +0.6 2,200,418 - Pf6N2E2_1855 0.62 +0.1 2,200,511 - Pf6N2E2_1855 0.70 +0.6 2,200,511 - Pf6N2E2_1855 0.70 -0.6 2,200,553 + Pf6N2E2_1855 0.74 +0.4 2,200,553 + Pf6N2E2_1855 0.74 +0.4 2,200,554 - Pf6N2E2_1855 0.74 -0.5 2,200,637 - Pf6N2E2_1855 0.81 +0.1 2,200,640 + Pf6N2E2_1855 0.81 -1.1 2,200,640 + Pf6N2E2_1855 0.81 +0.5 2,200,640 + Pf6N2E2_1855 0.81 +0.8 2,200,640 + Pf6N2E2_1855 0.81 +0.2 2,200,640 + Pf6N2E2_1855 0.81 -0.6 2,200,641 - Pf6N2E2_1855 0.81 -0.1 2,200,641 - Pf6N2E2_1855 0.81 -1.2 2,200,641 - Pf6N2E2_1855 0.81 -0.2 2,200,747 + +0.3 2,200,748 - +0.4 2,200,748 - -0.3 2,200,749 + -0.0 2,200,749 + -0.0 2,200,749 + -0.1 2,200,750 - -0.1 2,200,750 - +0.1 2,200,750 - -0.2 2,200,777 + +1.4 2,200,777 + -0.3 2,200,778 - -0.2 2,200,804 + +0.1 2,200,805 - +0.2 2,200,895 - +0.4 2,200,910 - +0.7 2,201,071 + Pf6N2E2_1856 0.14 -0.0 2,201,072 - Pf6N2E2_1856 0.14 -1.0 2,201,072 - Pf6N2E2_1856 0.14 -1.6 2,201,076 - Pf6N2E2_1856 0.14 -0.3 2,201,144 - Pf6N2E2_1856 0.19 +0.2 2,201,168 + Pf6N2E2_1856 0.20 +0.7 2,201,170 + Pf6N2E2_1856 0.21 +0.1 2,201,170 + Pf6N2E2_1856 0.21 -0.0 2,201,170 + Pf6N2E2_1856 0.21 +0.5 2,201,170 + Pf6N2E2_1856 0.21 +0.3 2,201,171 - Pf6N2E2_1856 0.21 -1.6 2,201,171 - Pf6N2E2_1856 0.21 -0.6 2,201,171 - Pf6N2E2_1856 0.21 -1.1 2,201,171 - Pf6N2E2_1856 0.21 -0.9 2,201,171 - Pf6N2E2_1856 0.21 -0.6 2,201,213 + Pf6N2E2_1856 0.24 -0.8 2,201,213 + Pf6N2E2_1856 0.24 -1.0 2,201,216 - Pf6N2E2_1856 0.24 +1.0 2,201,222 - Pf6N2E2_1856 0.24 -1.2 2,201,327 - Pf6N2E2_1856 0.32 +0.8 2,201,398 - Pf6N2E2_1856 0.37 -0.0 2,201,624 - Pf6N2E2_1856 0.53 -1.1 2,201,676 + Pf6N2E2_1856 0.57 +0.7 2,201,677 - Pf6N2E2_1856 0.57 -0.7 2,201,800 - Pf6N2E2_1856 0.65 -0.3 2,201,914 + Pf6N2E2_1856 0.73 -0.0 2,201,914 + Pf6N2E2_1856 0.73 +0.1 2,201,915 - Pf6N2E2_1856 0.73 -0.6 2,202,160 - -0.1 2,202,283 + +1.8 2,202,370 - +0.3 2,202,405 + +0.3 2,202,593 - Pf6N2E2_1857 0.17 +1.0 2,202,593 - Pf6N2E2_1857 0.17 -0.3 2,202,593 - Pf6N2E2_1857 0.17 -0.7 2,202,593 - Pf6N2E2_1857 0.17 +0.0 2,202,705 + Pf6N2E2_1857 0.40 -0.5 2,202,705 + Pf6N2E2_1857 0.40 +0.1 2,202,705 + Pf6N2E2_1857 0.40 +1.4 2,202,706 - Pf6N2E2_1857 0.40 -0.2 2,202,816 + Pf6N2E2_1857 0.63 +0.5 2,202,826 - Pf6N2E2_1857 0.65 -0.3 2,202,833 + Pf6N2E2_1857 0.67 +0.3 2,202,916 - Pf6N2E2_1857 0.84 +1.2 2,203,236 - Pf6N2E2_1858 0.39 +0.8 2,203,236 - Pf6N2E2_1858 0.39 -0.0
Or see this region's nucleotide sequence