Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01950 and MMP_RS01955 are separated by 188 nucleotides MMP_RS01955 and MMP_RS01960 are separated by 12 nucleotides MMP_RS01960 and MMP_RS01965 are separated by 18 nucleotides MMP_RS01965 and MMP_RS01970 are separated by 268 nucleotides
MMP_RS01950: MMP_RS01950 - hypothetical protein, at 361,472 to 363,307
_RS01950
MMP_RS01955: MMP_RS01955 - hypothetical protein, at 363,496 to 364,086
_RS01955
MMP_RS01960: MMP_RS01960 - hypothetical protein, at 364,099 to 364,338
_RS01960
MMP_RS01965: MMP_RS01965 - DUF1616 domain-containing protein, at 364,357 to 364,705
_RS01965
MMP_RS01970: MMP_RS01970 - TIGR04279 domain-containing protein, at 364,974 to 366,938
_RS01970
Position (kb) 363
364
365 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 363.101 kb on + strand, within MMP_RS01950 at 363.114 kb on - strand, within MMP_RS01950 at 363.115 kb on + strand, within MMP_RS01950 at 363.115 kb on + strand, within MMP_RS01950 at 363.122 kb on + strand, within MMP_RS01950 at 363.125 kb on + strand at 363.263 kb on - strand at 363.269 kb on + strand at 363.272 kb on - strand at 363.272 kb on - strand at 363.273 kb on + strand at 363.273 kb on + strand at 363.282 kb on + strand at 363.284 kb on - strand at 363.380 kb on - strand at 363.394 kb on + strand at 363.404 kb on + strand at 363.415 kb on + strand at 363.423 kb on - strand at 363.423 kb on - strand at 363.423 kb on - strand at 363.423 kb on - strand at 363.424 kb on + strand at 363.426 kb on + strand at 363.592 kb on - strand, within MMP_RS01955 at 363.593 kb on + strand, within MMP_RS01955 at 363.593 kb on + strand, within MMP_RS01955 at 363.827 kb on - strand, within MMP_RS01955 at 363.830 kb on - strand, within MMP_RS01955 at 364.269 kb on - strand, within MMP_RS01960 at 364.276 kb on - strand, within MMP_RS01960 at 364.321 kb on - strand at 364.321 kb on - strand at 364.322 kb on + strand at 364.324 kb on + strand at 364.324 kb on + strand at 364.326 kb on - strand at 364.466 kb on + strand, within MMP_RS01965 at 364.481 kb on + strand, within MMP_RS01965 at 364.555 kb on + strand, within MMP_RS01965 at 364.566 kb on - strand, within MMP_RS01965 at 364.589 kb on + strand, within MMP_RS01965 at 364.628 kb on + strand, within MMP_RS01965 at 364.676 kb on - strand at 364.838 kb on - strand at 364.838 kb on - strand at 364.839 kb on + strand at 364.843 kb on - strand at 364.898 kb on + strand at 364.899 kb on - strand at 364.900 kb on + strand at 364.905 kb on - strand at 364.906 kb on + strand at 364.908 kb on + strand at 364.916 kb on - strand at 364.917 kb on + strand at 364.917 kb on + strand at 364.917 kb on + strand at 364.917 kb on + strand at 364.918 kb on - strand at 364.918 kb on - strand at 364.918 kb on - strand at 364.918 kb on - strand at 364.918 kb on - strand at 364.918 kb on - strand at 364.919 kb on + strand at 364.919 kb on + strand at 364.919 kb on + strand at 364.919 kb on + strand at 364.919 kb on + strand at 364.922 kb on - strand at 364.923 kb on + strand at 364.931 kb on - strand at 364.949 kb on - strand at 364.949 kb on - strand at 364.949 kb on - strand at 364.949 kb on - strand at 364.958 kb on - strand at 364.971 kb on + strand at 365.012 kb on + strand at 365.023 kb on - strand at 365.023 kb on - strand at 365.024 kb on + strand at 365.027 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 363,101 + MMP_RS01950 0.89 -1.8 363,114 - MMP_RS01950 0.89 -1.1 363,115 + MMP_RS01950 0.89 -0.4 363,115 + MMP_RS01950 0.89 -3.5 363,122 + MMP_RS01950 0.90 -1.5 363,125 + -1.4 363,263 - -1.6 363,269 + -4.2 363,272 - -2.4 363,272 - -2.0 363,273 + -3.0 363,273 + -2.6 363,282 + -2.5 363,284 - -3.0 363,380 - -2.6 363,394 + -2.0 363,404 + -1.6 363,415 + -1.1 363,423 - -3.0 363,423 - -1.8 363,423 - -2.6 363,423 - -0.8 363,424 + -1.9 363,426 + -2.3 363,592 - MMP_RS01955 0.16 -2.5 363,593 + MMP_RS01955 0.16 -3.7 363,593 + MMP_RS01955 0.16 -1.3 363,827 - MMP_RS01955 0.56 -2.4 363,830 - MMP_RS01955 0.57 -0.8 364,269 - MMP_RS01960 0.71 -1.1 364,276 - MMP_RS01960 0.74 -2.0 364,321 - +0.1 364,321 - +1.1 364,322 + +1.3 364,324 + -1.8 364,324 + -2.6 364,326 - -2.7 364,466 + MMP_RS01965 0.31 -0.5 364,481 + MMP_RS01965 0.36 -0.1 364,555 + MMP_RS01965 0.57 +0.7 364,566 - MMP_RS01965 0.60 -0.6 364,589 + MMP_RS01965 0.66 -0.1 364,628 + MMP_RS01965 0.78 -1.7 364,676 - -0.6 364,838 - +0.1 364,838 - +0.4 364,839 + +0.3 364,843 - +0.3 364,898 + -1.6 364,899 - -2.3 364,900 + -0.3 364,905 - -1.9 364,906 + -3.0 364,908 + -1.6 364,916 - -2.7 364,917 + +0.9 364,917 + +1.3 364,917 + -1.7 364,917 + -1.8 364,918 - -3.0 364,918 - -3.4 364,918 - -2.2 364,918 - -1.5 364,918 - -2.9 364,918 - -0.3 364,919 + -1.6 364,919 + -1.8 364,919 + -2.2 364,919 + -2.1 364,919 + -2.6 364,922 - -1.3 364,923 + -1.8 364,931 - -2.1 364,949 - -1.3 364,949 - -2.4 364,949 - -1.8 364,949 - -2.1 364,958 - -2.9 364,971 + -2.9 365,012 + -1.6 365,023 - -1.6 365,023 - -3.0 365,024 + -3.1 365,027 - -2.2
Or see this region's nucleotide sequence