Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05433

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05431 and H281DRAFT_05432 overlap by 8 nucleotidesH281DRAFT_05432 and H281DRAFT_05433 are separated by 73 nucleotidesH281DRAFT_05433 and H281DRAFT_05434 are separated by 63 nucleotides H281DRAFT_05431: H281DRAFT_05431 - amino acid/amide ABC transporter membrane protein 2, HAAT family /amino acid/amide ABC transporter ATP-binding protein 1, HAAT family, at 6,731 to 8,593 _05431 H281DRAFT_05432: H281DRAFT_05432 - amino acid/amide ABC transporter membrane protein 1, HAAT family, at 8,586 to 9,419 _05432 H281DRAFT_05433: H281DRAFT_05433 - amino acid/amide ABC transporter substrate-binding protein, HAAT family (TC 3.A.1.4.-), at 9,493 to 10,656 _05433 H281DRAFT_05434: H281DRAFT_05434 - Predicted amidohydrolase, at 10,720 to 11,616 _05434 Position (kb) 9 10 11Strain fitness (log2 ratio) -2 -1 0 1 2 3at 8.823 kb on + strandat 8.823 kb on + strand, within H281DRAFT_05432at 8.824 kb on - strand, within H281DRAFT_05432at 8.979 kb on - strand, within H281DRAFT_05432at 9.257 kb on + strand, within H281DRAFT_05432at 9.258 kb on - strand, within H281DRAFT_05432at 9.258 kb on - strand, within H281DRAFT_05432at 9.289 kb on + strand, within H281DRAFT_05432at 9.338 kb on + strandat 9.357 kb on + strandat 9.358 kb on - strandat 9.374 kb on + strandat 9.374 kb on + strandat 9.454 kb on - strandat 9.454 kb on - strandat 9.717 kb on - strand, within H281DRAFT_05433at 9.839 kb on + strand, within H281DRAFT_05433at 9.854 kb on + strand, within H281DRAFT_05433at 9.854 kb on + strand, within H281DRAFT_05433at 9.855 kb on - strand, within H281DRAFT_05433at 9.918 kb on - strand, within H281DRAFT_05433at 10.064 kb on + strand, within H281DRAFT_05433at 10.064 kb on + strand, within H281DRAFT_05433at 10.125 kb on + strand, within H281DRAFT_05433at 10.161 kb on - strand, within H281DRAFT_05433at 10.161 kb on - strand, within H281DRAFT_05433at 10.241 kb on + strand, within H281DRAFT_05433at 10.241 kb on + strand, within H281DRAFT_05433at 10.271 kb on + strand, within H281DRAFT_05433at 10.282 kb on - strand, within H281DRAFT_05433at 10.282 kb on - strand, within H281DRAFT_05433at 10.283 kb on + strand, within H281DRAFT_05433at 10.284 kb on - strand, within H281DRAFT_05433at 10.287 kb on + strand, within H281DRAFT_05433at 10.287 kb on + strand, within H281DRAFT_05433at 10.287 kb on + strand, within H281DRAFT_05433at 10.647 kb on - strandat 11.008 kb on - strand, within H281DRAFT_05434at 11.064 kb on + strand, within H281DRAFT_05434at 11.113 kb on + strand, within H281DRAFT_05434at 11.135 kb on + strand, within H281DRAFT_05434at 11.135 kb on + strand, within H281DRAFT_05434at 11.136 kb on - strand, within H281DRAFT_05434at 11.169 kb on + strand, within H281DRAFT_05434at 11.574 kb on + strandat 11.575 kb on - strandat 11.575 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM
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8,823 + +2.7
8,823 + H281DRAFT_05432 0.28 -0.2
8,824 - H281DRAFT_05432 0.29 +0.3
8,979 - H281DRAFT_05432 0.47 -2.0
9,257 + H281DRAFT_05432 0.80 -0.5
9,258 - H281DRAFT_05432 0.81 +0.3
9,258 - H281DRAFT_05432 0.81 +0.0
9,289 + H281DRAFT_05432 0.84 -0.7
9,338 + +0.4
9,357 + +3.3
9,358 - -0.4
9,374 + -0.4
9,374 + -0.4
9,454 - +0.5
9,454 - +0.5
9,717 - H281DRAFT_05433 0.19 -0.5
9,839 + H281DRAFT_05433 0.30 -0.4
9,854 + H281DRAFT_05433 0.31 -0.3
9,854 + H281DRAFT_05433 0.31 -0.5
9,855 - H281DRAFT_05433 0.31 +0.6
9,918 - H281DRAFT_05433 0.37 -0.4
10,064 + H281DRAFT_05433 0.49 +0.1
10,064 + H281DRAFT_05433 0.49 +0.5
10,125 + H281DRAFT_05433 0.54 +0.4
10,161 - H281DRAFT_05433 0.57 -1.1
10,161 - H281DRAFT_05433 0.57 +0.2
10,241 + H281DRAFT_05433 0.64 +0.1
10,241 + H281DRAFT_05433 0.64 +0.6
10,271 + H281DRAFT_05433 0.67 +0.2
10,282 - H281DRAFT_05433 0.68 -1.1
10,282 - H281DRAFT_05433 0.68 -0.6
10,283 + H281DRAFT_05433 0.68 +1.4
10,284 - H281DRAFT_05433 0.68 -0.2
10,287 + H281DRAFT_05433 0.68 +0.0
10,287 + H281DRAFT_05433 0.68 -2.5
10,287 + H281DRAFT_05433 0.68 -0.3
10,647 - +0.3
11,008 - H281DRAFT_05434 0.32 -0.0
11,064 + H281DRAFT_05434 0.38 +0.6
11,113 + H281DRAFT_05434 0.44 +2.6
11,135 + H281DRAFT_05434 0.46 -1.1
11,135 + H281DRAFT_05434 0.46 -0.8
11,136 - H281DRAFT_05434 0.46 +0.8
11,169 + H281DRAFT_05434 0.50 -0.4
11,574 + -0.5
11,575 - -0.6
11,575 - +0.0

Or see this region's nucleotide sequence