Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04144 and H281DRAFT_04145 are separated by 112 nucleotides H281DRAFT_04145 and H281DRAFT_04146 overlap by 4 nucleotides H281DRAFT_04146 and H281DRAFT_04147 are separated by 46 nucleotides
H281DRAFT_04144: H281DRAFT_04144 - Tryptophan 2,3-dioxygenase holoenzyme /Tryptophan 2,3-dioxygenase apoenzyme, at 253,869 to 254,810
_04144
H281DRAFT_04145: H281DRAFT_04145 - NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family, at 254,923 to 255,660
_04145
H281DRAFT_04146: H281DRAFT_04146 - xylulokinase, at 255,657 to 257,201
_04146
H281DRAFT_04147: H281DRAFT_04147 - L-iditol 2-dehydrogenase, at 257,248 to 258,345
_04147
Position (kb) 255
256
257
258 Strain fitness (log2 ratio) -1
0
1
2 at 254.801 kb on + strand at 254.802 kb on - strand at 254.808 kb on + strand at 254.819 kb on + strand at 254.819 kb on + strand at 254.819 kb on + strand at 254.974 kb on - strand at 255.048 kb on + strand, within H281DRAFT_04145 at 255.103 kb on - strand, within H281DRAFT_04145 at 255.103 kb on - strand, within H281DRAFT_04145 at 255.103 kb on - strand, within H281DRAFT_04145 at 255.103 kb on - strand, within H281DRAFT_04145 at 255.103 kb on - strand, within H281DRAFT_04145 at 255.212 kb on + strand, within H281DRAFT_04145 at 255.651 kb on + strand at 255.652 kb on - strand at 255.652 kb on - strand at 255.712 kb on + strand at 255.734 kb on - strand at 255.881 kb on - strand, within H281DRAFT_04146 at 255.996 kb on + strand, within H281DRAFT_04146 at 256.307 kb on - strand, within H281DRAFT_04146 at 256.382 kb on - strand, within H281DRAFT_04146 at 256.796 kb on - strand, within H281DRAFT_04146 at 256.826 kb on - strand, within H281DRAFT_04146 at 256.894 kb on - strand, within H281DRAFT_04146 at 256.906 kb on + strand, within H281DRAFT_04146 at 256.919 kb on + strand, within H281DRAFT_04146 at 256.925 kb on + strand, within H281DRAFT_04146 at 257.104 kb on + strand at 257.104 kb on + strand at 257.194 kb on + strand at 257.215 kb on - strand at 257.216 kb on + strand at 257.270 kb on - strand at 257.568 kb on + strand, within H281DRAFT_04147 at 257.569 kb on - strand, within H281DRAFT_04147 at 257.570 kb on + strand, within H281DRAFT_04147 at 257.570 kb on + strand, within H281DRAFT_04147 at 257.570 kb on + strand, within H281DRAFT_04147 at 257.571 kb on - strand, within H281DRAFT_04147 at 257.572 kb on + strand, within H281DRAFT_04147 at 257.904 kb on + strand, within H281DRAFT_04147 at 257.905 kb on - strand, within H281DRAFT_04147 at 258.000 kb on - strand, within H281DRAFT_04147
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM remove 254,801 + +0.5 254,802 - -0.8 254,808 + -0.8 254,819 + +0.5 254,819 + -0.6 254,819 + -0.1 254,974 - -1.1 255,048 + H281DRAFT_04145 0.17 +0.5 255,103 - H281DRAFT_04145 0.24 -0.0 255,103 - H281DRAFT_04145 0.24 -1.5 255,103 - H281DRAFT_04145 0.24 +0.6 255,103 - H281DRAFT_04145 0.24 +0.2 255,103 - H281DRAFT_04145 0.24 -0.1 255,212 + H281DRAFT_04145 0.39 -0.4 255,651 + -0.0 255,652 - -0.5 255,652 - +0.9 255,712 + -0.2 255,734 - +0.1 255,881 - H281DRAFT_04146 0.14 -0.1 255,996 + H281DRAFT_04146 0.22 +2.1 256,307 - H281DRAFT_04146 0.42 +0.6 256,382 - H281DRAFT_04146 0.47 -1.2 256,796 - H281DRAFT_04146 0.74 +0.2 256,826 - H281DRAFT_04146 0.76 -0.4 256,894 - H281DRAFT_04146 0.80 +1.1 256,906 + H281DRAFT_04146 0.81 +0.1 256,919 + H281DRAFT_04146 0.82 +0.2 256,925 + H281DRAFT_04146 0.82 +0.2 257,104 + +0.9 257,104 + +0.1 257,194 + -1.7 257,215 - -0.7 257,216 + -1.3 257,270 - +0.6 257,568 + H281DRAFT_04147 0.29 -0.3 257,569 - H281DRAFT_04147 0.29 -0.2 257,570 + H281DRAFT_04147 0.29 +2.0 257,570 + H281DRAFT_04147 0.29 +0.7 257,570 + H281DRAFT_04147 0.29 +0.8 257,571 - H281DRAFT_04147 0.29 -0.8 257,572 + H281DRAFT_04147 0.30 +0.8 257,904 + H281DRAFT_04147 0.60 +1.4 257,905 - H281DRAFT_04147 0.60 +0.0 258,000 - H281DRAFT_04147 0.68 +0.3
Or see this region's nucleotide sequence