Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04062 and H281DRAFT_04063 are separated by 229 nucleotides H281DRAFT_04063 and H281DRAFT_04064 are separated by 535 nucleotides
H281DRAFT_04062: H281DRAFT_04062 - amino acid/amide ABC transporter membrane protein 1, HAAT family, at 163,181 to 164,131
_04062
H281DRAFT_04063: H281DRAFT_04063 - amino acid/amide ABC transporter substrate-binding protein, HAAT family, at 164,361 to 165,563
_04063
H281DRAFT_04064: H281DRAFT_04064 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 166,099 to 167,058
_04064
Position (kb)
164
165
166 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 163.434 kb on + strand, within H281DRAFT_04062 at 163.435 kb on - strand, within H281DRAFT_04062 at 163.875 kb on + strand, within H281DRAFT_04062 at 163.970 kb on + strand, within H281DRAFT_04062 at 163.971 kb on - strand, within H281DRAFT_04062 at 164.046 kb on + strand at 164.056 kb on + strand at 164.056 kb on + strand at 164.056 kb on + strand at 164.057 kb on - strand at 164.057 kb on - strand at 164.057 kb on - strand at 164.057 kb on - strand at 164.079 kb on + strand at 164.079 kb on + strand at 164.079 kb on + strand at 164.080 kb on - strand at 164.106 kb on - strand at 164.125 kb on + strand at 164.125 kb on + strand at 164.126 kb on - strand at 164.133 kb on + strand at 164.134 kb on - strand at 164.134 kb on - strand at 164.143 kb on + strand at 164.229 kb on + strand at 164.229 kb on + strand at 164.363 kb on - strand at 164.363 kb on - strand at 164.410 kb on + strand at 164.411 kb on - strand at 164.411 kb on - strand at 164.411 kb on - strand at 164.411 kb on - strand at 164.417 kb on + strand at 164.480 kb on - strand at 164.480 kb on - strand at 164.557 kb on + strand, within H281DRAFT_04063 at 164.557 kb on + strand, within H281DRAFT_04063 at 164.558 kb on - strand, within H281DRAFT_04063 at 164.569 kb on + strand, within H281DRAFT_04063 at 164.569 kb on + strand, within H281DRAFT_04063 at 164.570 kb on - strand, within H281DRAFT_04063 at 164.570 kb on - strand, within H281DRAFT_04063 at 164.570 kb on - strand, within H281DRAFT_04063 at 164.584 kb on + strand, within H281DRAFT_04063 at 164.584 kb on + strand, within H281DRAFT_04063 at 164.585 kb on - strand, within H281DRAFT_04063 at 164.585 kb on - strand, within H281DRAFT_04063 at 164.617 kb on + strand, within H281DRAFT_04063 at 164.617 kb on + strand, within H281DRAFT_04063 at 164.617 kb on + strand, within H281DRAFT_04063 at 164.618 kb on - strand, within H281DRAFT_04063 at 164.618 kb on - strand, within H281DRAFT_04063 at 164.727 kb on - strand, within H281DRAFT_04063 at 165.071 kb on - strand, within H281DRAFT_04063 at 165.148 kb on + strand, within H281DRAFT_04063 at 165.149 kb on - strand, within H281DRAFT_04063 at 165.149 kb on - strand at 165.149 kb on - strand, within H281DRAFT_04063 at 165.568 kb on + strand at 165.569 kb on - strand at 165.569 kb on - strand at 165.569 kb on - strand at 165.569 kb on - strand at 165.593 kb on + strand at 165.633 kb on + strand at 165.634 kb on - strand at 165.634 kb on - strand at 165.657 kb on + strand at 165.658 kb on - strand at 165.658 kb on - strand at 165.835 kb on - strand at 165.845 kb on + strand at 165.845 kb on + strand at 165.845 kb on + strand at 165.877 kb on + strand at 165.878 kb on - strand at 165.878 kb on - strand at 165.890 kb on + strand at 165.890 kb on + strand at 165.891 kb on - strand at 165.916 kb on - strand at 165.945 kb on + strand at 166.086 kb on + strand at 166.087 kb on - strand at 166.308 kb on + strand, within H281DRAFT_04064 at 166.309 kb on - strand at 166.310 kb on + strand, within H281DRAFT_04064 at 166.310 kb on + strand, within H281DRAFT_04064 at 166.310 kb on + strand, within H281DRAFT_04064 at 166.311 kb on - strand, within H281DRAFT_04064 at 166.311 kb on - strand, within H281DRAFT_04064 at 166.311 kb on - strand at 166.445 kb on + strand, within H281DRAFT_04064 at 166.445 kb on + strand at 166.445 kb on + strand at 166.485 kb on - strand, within H281DRAFT_04064 at 166.485 kb on - strand, within H281DRAFT_04064
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM remove 163,434 + H281DRAFT_04062 0.27 +0.5 163,435 - H281DRAFT_04062 0.27 +0.3 163,875 + H281DRAFT_04062 0.73 -1.1 163,970 + H281DRAFT_04062 0.83 -0.3 163,971 - H281DRAFT_04062 0.83 +0.7 164,046 + +0.1 164,056 + -1.1 164,056 + -1.3 164,056 + +0.5 164,057 - +1.9 164,057 - +0.2 164,057 - -0.2 164,057 - -0.8 164,079 + -1.3 164,079 + +1.3 164,079 + +1.2 164,080 - +0.8 164,106 - -0.1 164,125 + -0.1 164,125 + +0.7 164,126 - +0.5 164,133 + +1.6 164,134 - +0.1 164,134 - +0.5 164,143 + +2.5 164,229 + -1.8 164,229 + -0.4 164,363 - +0.4 164,363 - -0.3 164,410 + -0.1 164,411 - +0.1 164,411 - -0.3 164,411 - +0.8 164,411 - +0.7 164,417 + -0.5 164,480 - +1.0 164,480 - +0.3 164,557 + H281DRAFT_04063 0.16 -0.2 164,557 + H281DRAFT_04063 0.16 -2.2 164,558 - H281DRAFT_04063 0.16 -0.2 164,569 + H281DRAFT_04063 0.17 -0.9 164,569 + H281DRAFT_04063 0.17 +0.5 164,570 - H281DRAFT_04063 0.17 -1.2 164,570 - H281DRAFT_04063 0.17 -0.1 164,570 - H281DRAFT_04063 0.17 +0.2 164,584 + H281DRAFT_04063 0.19 +0.1 164,584 + H281DRAFT_04063 0.19 +0.0 164,585 - H281DRAFT_04063 0.19 +0.3 164,585 - H281DRAFT_04063 0.19 +0.4 164,617 + H281DRAFT_04063 0.21 -1.4 164,617 + H281DRAFT_04063 0.21 +0.4 164,617 + H281DRAFT_04063 0.21 -0.2 164,618 - H281DRAFT_04063 0.21 +0.4 164,618 - H281DRAFT_04063 0.21 +0.1 164,727 - H281DRAFT_04063 0.30 +0.7 165,071 - H281DRAFT_04063 0.59 -0.0 165,148 + H281DRAFT_04063 0.65 -0.6 165,149 - H281DRAFT_04063 0.66 +1.9 165,149 - +1.3 165,149 - H281DRAFT_04063 0.66 -0.9 165,568 + -2.3 165,569 - +0.3 165,569 - -0.1 165,569 - +1.3 165,569 - +0.7 165,593 + +0.1 165,633 + +0.8 165,634 - +0.3 165,634 - +0.6 165,657 + +0.4 165,658 - +0.4 165,658 - -0.1 165,835 - -0.4 165,845 + +0.7 165,845 + +0.1 165,845 + +0.3 165,877 + +0.1 165,878 - +0.1 165,878 - +0.3 165,890 + -0.2 165,890 + +0.1 165,891 - -0.1 165,916 - +0.9 165,945 + +1.9 166,086 + +0.1 166,087 - +0.2 166,308 + H281DRAFT_04064 0.22 -1.7 166,309 - -0.7 166,310 + H281DRAFT_04064 0.22 -1.1 166,310 + H281DRAFT_04064 0.22 -1.7 166,310 + H281DRAFT_04064 0.22 -1.5 166,311 - H281DRAFT_04064 0.22 -0.1 166,311 - H281DRAFT_04064 0.22 -2.4 166,311 - -0.7 166,445 + H281DRAFT_04064 0.36 -1.7 166,445 + +3.7 166,445 + +1.9 166,485 - H281DRAFT_04064 0.40 +0.4 166,485 - H281DRAFT_04064 0.40 -0.7
Or see this region's nucleotide sequence