Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_03214

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_03212 and H281DRAFT_03213 are separated by 164 nucleotidesH281DRAFT_03213 and H281DRAFT_03214 are separated by 306 nucleotidesH281DRAFT_03214 and H281DRAFT_03215 are separated by 463 nucleotidesH281DRAFT_03215 and H281DRAFT_03216 overlap by 4 nucleotides H281DRAFT_03212: H281DRAFT_03212 - hypothetical protein, at 259,577 to 259,837 _03212 H281DRAFT_03213: H281DRAFT_03213 - hypothetical protein, at 260,002 to 260,334 _03213 H281DRAFT_03214: H281DRAFT_03214 - 4-hydroxyphenylpyruvate dioxygenase, at 260,641 to 262,524 _03214 H281DRAFT_03215: H281DRAFT_03215 - 3-dehydroquinate dehydratase, at 262,988 to 263,443 _03215 H281DRAFT_03216: H281DRAFT_03216 - shikimate dehydrogenase (EC 1.1.1.25), at 263,440 to 264,342 _03216 Position (kb) 260 261 262 263Strain fitness (log2 ratio) -2 -1 0 1at 259.817 kb on - strandat 260.067 kb on - strand, within H281DRAFT_03213at 260.075 kb on + strand, within H281DRAFT_03213at 260.176 kb on - strand, within H281DRAFT_03213at 260.232 kb on + strand, within H281DRAFT_03213at 260.334 kb on + strandat 260.334 kb on + strandat 260.335 kb on - strandat 260.486 kb on + strandat 260.498 kb on - strandat 260.498 kb on - strandat 260.763 kb on - strandat 260.809 kb on - strandat 260.876 kb on + strand, within H281DRAFT_03214at 260.969 kb on - strand, within H281DRAFT_03214at 260.988 kb on + strand, within H281DRAFT_03214at 260.989 kb on - strand, within H281DRAFT_03214at 261.302 kb on + strand, within H281DRAFT_03214at 261.380 kb on + strand, within H281DRAFT_03214at 261.531 kb on - strand, within H281DRAFT_03214at 261.677 kb on + strand, within H281DRAFT_03214at 261.805 kb on + strand, within H281DRAFT_03214at 261.806 kb on - strand, within H281DRAFT_03214at 261.830 kb on + strand, within H281DRAFT_03214at 262.061 kb on - strand, within H281DRAFT_03214at 262.231 kb on + strand, within H281DRAFT_03214at 262.318 kb on + strand, within H281DRAFT_03214at 262.413 kb on - strandat 262.480 kb on + strandat 262.699 kb on + strandat 262.896 kb on + strandat 262.946 kb on - strandat 263.004 kb on + strandat 263.226 kb on - strand, within H281DRAFT_03215at 263.294 kb on - strand, within H281DRAFT_03215at 263.336 kb on + strand, within H281DRAFT_03215at 263.337 kb on - strand, within H281DRAFT_03215at 263.388 kb on - strand, within H281DRAFT_03215

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM
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259,817 - -0.2
260,067 - H281DRAFT_03213 0.20 -0.5
260,075 + H281DRAFT_03213 0.22 -1.7
260,176 - H281DRAFT_03213 0.52 -0.6
260,232 + H281DRAFT_03213 0.69 +0.2
260,334 + +0.1
260,334 + -0.3
260,335 - +0.4
260,486 + +0.3
260,498 - +1.5
260,498 - +0.8
260,763 - -1.0
260,809 - +1.0
260,876 + H281DRAFT_03214 0.12 -0.3
260,969 - H281DRAFT_03214 0.17 -0.1
260,988 + H281DRAFT_03214 0.18 +1.0
260,989 - H281DRAFT_03214 0.18 +1.0
261,302 + H281DRAFT_03214 0.35 +0.4
261,380 + H281DRAFT_03214 0.39 +0.4
261,531 - H281DRAFT_03214 0.47 +0.5
261,677 + H281DRAFT_03214 0.55 +0.2
261,805 + H281DRAFT_03214 0.62 +1.4
261,806 - H281DRAFT_03214 0.62 -1.3
261,830 + H281DRAFT_03214 0.63 +1.2
262,061 - H281DRAFT_03214 0.75 +0.0
262,231 + H281DRAFT_03214 0.84 -0.3
262,318 + H281DRAFT_03214 0.89 +1.1
262,413 - +0.1
262,480 + +0.2
262,699 + -2.1
262,896 + +0.8
262,946 - +0.4
263,004 + -0.1
263,226 - H281DRAFT_03215 0.52 -0.1
263,294 - H281DRAFT_03215 0.67 +0.4
263,336 + H281DRAFT_03215 0.76 -0.1
263,337 - H281DRAFT_03215 0.77 +1.1
263,388 - H281DRAFT_03215 0.88 +0.5

Or see this region's nucleotide sequence