Experiment: PYE with Nalidixic 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00422 and CCNA_03919 are separated by 1 nucleotides CCNA_03919 and CCNA_00423 are separated by 233 nucleotides CCNA_00423 and CCNA_00424 are separated by 246 nucleotides
CCNA_00422: CCNA_00422 - SDR pteridine reductase, at 432,336 to 433,088
_00422
CCNA_03919: CCNA_03919 - hypothetical protein, at 433,090 to 433,170
_03919
CCNA_00423: CCNA_00423 - phosphohydrolase, MutT-nudix family, at 433,404 to 433,874
_00423
CCNA_00424: CCNA_00424 - aldehyde dehydrogenase, at 434,121 to 435,560
_00424
Position (kb) 433
434 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 432.404 kb on - strand at 432.435 kb on - strand, within CCNA_00422 at 432.452 kb on + strand, within CCNA_00422 at 432.460 kb on - strand, within CCNA_00422 at 432.533 kb on + strand, within CCNA_00422 at 432.536 kb on - strand, within CCNA_00422 at 432.545 kb on + strand, within CCNA_00422 at 432.587 kb on - strand, within CCNA_00422 at 432.588 kb on - strand at 432.591 kb on + strand, within CCNA_00422 at 432.591 kb on + strand, within CCNA_00422 at 432.598 kb on - strand, within CCNA_00422 at 432.616 kb on + strand, within CCNA_00422 at 432.621 kb on + strand, within CCNA_00422 at 432.626 kb on + strand, within CCNA_00422 at 432.724 kb on + strand, within CCNA_00422 at 432.732 kb on - strand, within CCNA_00422 at 432.760 kb on - strand, within CCNA_00422 at 432.793 kb on + strand, within CCNA_00422 at 432.824 kb on - strand, within CCNA_00422 at 432.903 kb on - strand, within CCNA_00422 at 432.920 kb on - strand, within CCNA_00422 at 432.944 kb on - strand, within CCNA_00422 at 432.963 kb on + strand, within CCNA_00422 at 432.984 kb on + strand, within CCNA_00422 at 433.007 kb on - strand, within CCNA_00422 at 433.039 kb on - strand at 433.062 kb on + strand at 433.067 kb on + strand at 433.070 kb on - strand at 433.070 kb on - strand at 433.070 kb on - strand at 433.070 kb on - strand at 433.075 kb on - strand at 433.102 kb on + strand, within CCNA_03919 at 433.116 kb on + strand, within CCNA_03919 at 433.124 kb on - strand, within CCNA_03919 at 433.125 kb on - strand, within CCNA_03919 at 433.136 kb on + strand, within CCNA_03919 at 433.136 kb on + strand, within CCNA_03919 at 433.144 kb on - strand, within CCNA_03919 at 433.144 kb on - strand, within CCNA_03919 at 433.148 kb on - strand, within CCNA_03919 at 433.172 kb on + strand at 433.205 kb on - strand at 433.233 kb on - strand at 433.245 kb on + strand at 433.253 kb on - strand at 433.264 kb on + strand at 433.268 kb on - strand at 433.268 kb on - strand at 433.304 kb on + strand at 433.306 kb on + strand at 433.306 kb on + strand at 433.306 kb on + strand at 433.306 kb on + strand at 433.306 kb on + strand at 433.319 kb on + strand at 433.338 kb on + strand at 433.338 kb on + strand at 433.338 kb on + strand at 433.338 kb on + strand at 433.346 kb on - strand at 433.346 kb on - strand at 433.378 kb on + strand at 433.390 kb on + strand at 433.390 kb on + strand at 433.397 kb on - strand at 433.406 kb on + strand at 433.406 kb on + strand at 433.414 kb on - strand at 433.445 kb on + strand at 433.445 kb on + strand at 433.452 kb on + strand, within CCNA_00423 at 433.453 kb on - strand, within CCNA_00423 at 433.453 kb on - strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.482 kb on - strand, within CCNA_00423 at 433.514 kb on + strand, within CCNA_00423 at 433.514 kb on + strand, within CCNA_00423 at 433.522 kb on - strand, within CCNA_00423 at 433.538 kb on + strand, within CCNA_00423 at 433.541 kb on - strand, within CCNA_00423 at 433.546 kb on - strand, within CCNA_00423 at 433.552 kb on - strand, within CCNA_00423 at 433.559 kb on - strand, within CCNA_00423 at 433.560 kb on - strand, within CCNA_00423 at 433.668 kb on + strand, within CCNA_00423 at 433.683 kb on + strand, within CCNA_00423 at 433.696 kb on - strand, within CCNA_00423 at 433.707 kb on + strand, within CCNA_00423 at 433.707 kb on + strand, within CCNA_00423 at 433.741 kb on + strand, within CCNA_00423 at 433.749 kb on - strand, within CCNA_00423 at 433.815 kb on - strand, within CCNA_00423 at 433.839 kb on + strand at 433.844 kb on + strand at 433.846 kb on + strand at 433.851 kb on + strand at 433.851 kb on + strand at 433.855 kb on + strand at 433.859 kb on - strand at 433.863 kb on - strand at 433.870 kb on + strand at 433.873 kb on - strand at 433.875 kb on + strand at 433.878 kb on + strand at 433.899 kb on - strand at 433.915 kb on - strand at 433.925 kb on - strand at 433.926 kb on + strand at 433.949 kb on + strand at 433.949 kb on + strand at 433.949 kb on + strand at 433.949 kb on + strand at 433.949 kb on + strand at 433.949 kb on + strand at 433.957 kb on - strand at 433.957 kb on - strand at 433.957 kb on - strand at 433.957 kb on - strand at 433.957 kb on - strand at 433.957 kb on - strand at 433.965 kb on - strand at 433.965 kb on - strand at 433.965 kb on - strand at 433.996 kb on + strand at 433.997 kb on - strand at 434.033 kb on + strand at 434.041 kb on - strand at 434.041 kb on - strand at 434.041 kb on - strand at 434.060 kb on + strand at 434.105 kb on + strand at 434.105 kb on + strand at 434.105 kb on + strand at 434.105 kb on + strand at 434.105 kb on + strand at 434.113 kb on - strand at 434.113 kb on - strand at 434.113 kb on - strand at 434.128 kb on + strand at 434.138 kb on + strand at 434.146 kb on - strand at 434.206 kb on + strand at 434.213 kb on + strand at 434.213 kb on + strand at 434.247 kb on + strand at 434.258 kb on + strand at 434.260 kb on - strand at 434.282 kb on - strand, within CCNA_00424 at 434.288 kb on - strand, within CCNA_00424 at 434.296 kb on + strand, within CCNA_00424 at 434.303 kb on - strand, within CCNA_00424 at 434.305 kb on + strand, within CCNA_00424 at 434.305 kb on + strand, within CCNA_00424 at 434.305 kb on + strand, within CCNA_00424 at 434.357 kb on - strand, within CCNA_00424 at 434.372 kb on + strand, within CCNA_00424 at 434.372 kb on + strand, within CCNA_00424 at 434.372 kb on + strand, within CCNA_00424 at 434.372 kb on + strand, within CCNA_00424 at 434.372 kb on + strand, within CCNA_00424 at 434.386 kb on - strand, within CCNA_00424 at 434.410 kb on + strand, within CCNA_00424 at 434.410 kb on + strand, within CCNA_00424 at 434.434 kb on + strand, within CCNA_00424 at 434.446 kb on + strand, within CCNA_00424 at 434.451 kb on + strand, within CCNA_00424 at 434.451 kb on + strand, within CCNA_00424 at 434.451 kb on + strand, within CCNA_00424 at 434.454 kb on - strand, within CCNA_00424 at 434.454 kb on - strand, within CCNA_00424 at 434.458 kb on - strand, within CCNA_00424 at 434.459 kb on - strand, within CCNA_00424 at 434.471 kb on + strand, within CCNA_00424 at 434.523 kb on + strand, within CCNA_00424 at 434.549 kb on - strand, within CCNA_00424 at 434.553 kb on + strand, within CCNA_00424 at 434.561 kb on - strand, within CCNA_00424 at 434.622 kb on + strand, within CCNA_00424 at 434.624 kb on + strand, within CCNA_00424 at 434.624 kb on + strand, within CCNA_00424 at 434.624 kb on + strand, within CCNA_00424 at 434.631 kb on + strand, within CCNA_00424 at 434.632 kb on - strand, within CCNA_00424 at 434.691 kb on - strand at 434.713 kb on - strand, within CCNA_00424 at 434.714 kb on + strand, within CCNA_00424 at 434.722 kb on - strand, within CCNA_00424 at 434.795 kb on - strand, within CCNA_00424 at 434.803 kb on + strand, within CCNA_00424 at 434.814 kb on + strand, within CCNA_00424 at 434.847 kb on + strand, within CCNA_00424 at 434.848 kb on + strand, within CCNA_00424 at 434.848 kb on + strand, within CCNA_00424 at 434.853 kb on + strand, within CCNA_00424 at 434.856 kb on - strand, within CCNA_00424 at 434.870 kb on + strand, within CCNA_00424 at 434.871 kb on + strand, within CCNA_00424
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Nalidixic 0.06 mg/ml remove 432,404 - -1.4 432,435 - CCNA_00422 0.13 -1.0 432,452 + CCNA_00422 0.15 +0.1 432,460 - CCNA_00422 0.16 +0.6 432,533 + CCNA_00422 0.26 -1.3 432,536 - CCNA_00422 0.27 -2.0 432,545 + CCNA_00422 0.28 +0.0 432,587 - CCNA_00422 0.33 -1.4 432,588 - +1.6 432,591 + CCNA_00422 0.34 -0.4 432,591 + CCNA_00422 0.34 -3.0 432,598 - CCNA_00422 0.35 -0.7 432,616 + CCNA_00422 0.37 +0.4 432,621 + CCNA_00422 0.38 -1.2 432,626 + CCNA_00422 0.39 -1.1 432,724 + CCNA_00422 0.52 -0.3 432,732 - CCNA_00422 0.53 -2.1 432,760 - CCNA_00422 0.56 -3.3 432,793 + CCNA_00422 0.61 +0.9 432,824 - CCNA_00422 0.65 -1.4 432,903 - CCNA_00422 0.75 -0.7 432,920 - CCNA_00422 0.78 +0.5 432,944 - CCNA_00422 0.81 -0.1 432,963 + CCNA_00422 0.83 +1.2 432,984 + CCNA_00422 0.86 -0.4 433,007 - CCNA_00422 0.89 -3.1 433,039 - -2.2 433,062 + +1.7 433,067 + -0.4 433,070 - -0.7 433,070 - -4.0 433,070 - +0.4 433,070 - +0.9 433,075 - +1.0 433,102 + CCNA_03919 0.15 +0.2 433,116 + CCNA_03919 0.32 +1.8 433,124 - CCNA_03919 0.42 -0.2 433,125 - CCNA_03919 0.43 -2.7 433,136 + CCNA_03919 0.57 -0.2 433,136 + CCNA_03919 0.57 -0.4 433,144 - CCNA_03919 0.67 +1.2 433,144 - CCNA_03919 0.67 +1.3 433,148 - CCNA_03919 0.72 +2.2 433,172 + -0.5 433,205 - +2.9 433,233 - -0.5 433,245 + +3.9 433,253 - -1.0 433,264 + -1.2 433,268 - +0.8 433,268 - +0.4 433,304 + -1.0 433,306 + -0.5 433,306 + -1.0 433,306 + -1.5 433,306 + -0.2 433,306 + -1.3 433,319 + +0.4 433,338 + -1.5 433,338 + +1.3 433,338 + -0.7 433,338 + -1.5 433,346 - -0.3 433,346 - +1.8 433,378 + -2.4 433,390 + +1.9 433,390 + +0.2 433,397 - +0.4 433,406 + +1.3 433,406 + +0.9 433,414 - -1.2 433,445 + -0.8 433,445 + +1.3 433,452 + CCNA_00423 0.10 -0.6 433,453 - CCNA_00423 0.10 +0.2 433,453 - CCNA_00423 0.10 -0.8 433,474 + CCNA_00423 0.15 +0.3 433,474 + CCNA_00423 0.15 -0.6 433,474 + CCNA_00423 0.15 +0.6 433,474 + CCNA_00423 0.15 -0.7 433,474 + CCNA_00423 0.15 +1.6 433,474 + CCNA_00423 0.15 -0.4 433,474 + CCNA_00423 0.15 +1.8 433,482 - CCNA_00423 0.17 +0.0 433,514 + CCNA_00423 0.23 -1.3 433,514 + CCNA_00423 0.23 -1.0 433,522 - CCNA_00423 0.25 +0.6 433,538 + CCNA_00423 0.28 -2.0 433,541 - CCNA_00423 0.29 +0.5 433,546 - CCNA_00423 0.30 +0.9 433,552 - CCNA_00423 0.31 +0.4 433,559 - CCNA_00423 0.33 -0.4 433,560 - CCNA_00423 0.33 -1.8 433,668 + CCNA_00423 0.56 +0.8 433,683 + CCNA_00423 0.59 -0.7 433,696 - CCNA_00423 0.62 -1.2 433,707 + CCNA_00423 0.64 -2.9 433,707 + CCNA_00423 0.64 +0.5 433,741 + CCNA_00423 0.72 +2.6 433,749 - CCNA_00423 0.73 -1.6 433,815 - CCNA_00423 0.87 -0.7 433,839 + -1.9 433,844 + +1.9 433,846 + -0.2 433,851 + +0.4 433,851 + -0.8 433,855 + -2.0 433,859 - +0.5 433,863 - +3.0 433,870 + +3.3 433,873 - +0.5 433,875 + +1.0 433,878 + -1.8 433,899 - -1.9 433,915 - +0.3 433,925 - -0.3 433,926 + +0.8 433,949 + -0.9 433,949 + -1.4 433,949 + +0.0 433,949 + -1.7 433,949 + +0.0 433,949 + -3.4 433,957 - -1.4 433,957 - -0.7 433,957 - +1.2 433,957 - +0.4 433,957 - +1.8 433,957 - -0.6 433,965 - -0.2 433,965 - +1.2 433,965 - +0.0 433,996 + -0.6 433,997 - -2.4 434,033 + +1.2 434,041 - -1.2 434,041 - -1.1 434,041 - +1.2 434,060 + -1.3 434,105 + +1.6 434,105 + -2.7 434,105 + +1.9 434,105 + -1.5 434,105 + -2.0 434,113 - -0.1 434,113 - +0.9 434,113 - -0.1 434,128 + +0.9 434,138 + +0.4 434,146 - +1.5 434,206 + -0.7 434,213 + -1.9 434,213 + +2.3 434,247 + -2.1 434,258 + +1.2 434,260 - +2.0 434,282 - CCNA_00424 0.11 +3.2 434,288 - CCNA_00424 0.12 +0.2 434,296 + CCNA_00424 0.12 -0.3 434,303 - CCNA_00424 0.13 +0.4 434,305 + CCNA_00424 0.13 -1.5 434,305 + CCNA_00424 0.13 -0.2 434,305 + CCNA_00424 0.13 +2.7 434,357 - CCNA_00424 0.16 +1.4 434,372 + CCNA_00424 0.17 +1.0 434,372 + CCNA_00424 0.17 -2.0 434,372 + CCNA_00424 0.17 -0.2 434,372 + CCNA_00424 0.17 +1.4 434,372 + CCNA_00424 0.17 -2.1 434,386 - CCNA_00424 0.18 -1.4 434,410 + CCNA_00424 0.20 +2.5 434,410 + CCNA_00424 0.20 -0.8 434,434 + CCNA_00424 0.22 +0.6 434,446 + CCNA_00424 0.23 +0.7 434,451 + CCNA_00424 0.23 -0.6 434,451 + CCNA_00424 0.23 +1.1 434,451 + CCNA_00424 0.23 +1.1 434,454 - CCNA_00424 0.23 -0.5 434,454 - CCNA_00424 0.23 +0.6 434,458 - CCNA_00424 0.23 -1.9 434,459 - CCNA_00424 0.23 -0.7 434,471 + CCNA_00424 0.24 +2.1 434,523 + CCNA_00424 0.28 +2.8 434,549 - CCNA_00424 0.30 -2.1 434,553 + CCNA_00424 0.30 +0.4 434,561 - CCNA_00424 0.31 +1.6 434,622 + CCNA_00424 0.35 -1.1 434,624 + CCNA_00424 0.35 +1.2 434,624 + CCNA_00424 0.35 -2.0 434,624 + CCNA_00424 0.35 +1.9 434,631 + CCNA_00424 0.35 +0.5 434,632 - CCNA_00424 0.35 +0.7 434,691 - +3.9 434,713 - CCNA_00424 0.41 +2.9 434,714 + CCNA_00424 0.41 -0.4 434,722 - CCNA_00424 0.42 +0.0 434,795 - CCNA_00424 0.47 +2.7 434,803 + CCNA_00424 0.47 +1.7 434,814 + CCNA_00424 0.48 -2.3 434,847 + CCNA_00424 0.50 +1.9 434,848 + CCNA_00424 0.50 -1.4 434,848 + CCNA_00424 0.50 -2.3 434,853 + CCNA_00424 0.51 +1.3 434,856 - CCNA_00424 0.51 +1.1 434,870 + CCNA_00424 0.52 +2.4 434,871 + CCNA_00424 0.52 +0.3
Or see this region's nucleotide sequence