Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20170

Experiment: NAG (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20165 and BPHYT_RS20170 are separated by 168 nucleotidesBPHYT_RS20170 and BPHYT_RS20175 are separated by 56 nucleotidesBPHYT_RS20175 and BPHYT_RS20180 overlap by 4 nucleotides BPHYT_RS20165: BPHYT_RS20165 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 66,730 to 67,968 _RS20165 BPHYT_RS20170: BPHYT_RS20170 - sugar transporter, at 68,137 to 70,359 _RS20170 BPHYT_RS20175: BPHYT_RS20175 - protein-tyrosine-phosphatase, at 70,416 to 70,868 _RS20175 BPHYT_RS20180: BPHYT_RS20180 - sugar ABC transporter substrate-binding protein, at 70,865 to 71,917 _RS20180 Position (kb) 68 69 70 71Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 67.514 kb on - strand, within BPHYT_RS20165at 67.676 kb on - strand, within BPHYT_RS20165at 67.728 kb on + strand, within BPHYT_RS20165at 68.162 kb on + strandat 68.450 kb on - strand, within BPHYT_RS20170at 68.727 kb on + strand, within BPHYT_RS20170at 69.303 kb on - strand, within BPHYT_RS20170at 69.871 kb on - strand, within BPHYT_RS20170at 70.860 kb on - strandat 70.889 kb on + strandat 71.069 kb on + strand, within BPHYT_RS20180at 71.182 kb on + strand, within BPHYT_RS20180at 71.251 kb on + strand, within BPHYT_RS20180

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Per-strain Table

Position Strand Gene LocusTag Fraction NAG (N)
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67,514 - BPHYT_RS20165 0.63 -0.8
67,676 - BPHYT_RS20165 0.76 -0.4
67,728 + BPHYT_RS20165 0.81 +1.2
68,162 + -1.9
68,450 - BPHYT_RS20170 0.14 -2.6
68,727 + BPHYT_RS20170 0.27 -0.6
69,303 - BPHYT_RS20170 0.52 -1.1
69,871 - BPHYT_RS20170 0.78 -0.6
70,860 - +0.2
70,889 + +1.2
71,069 + BPHYT_RS20180 0.19 -0.2
71,182 + BPHYT_RS20180 0.30 +5.1
71,251 + BPHYT_RS20180 0.37 -0.4

Or see this region's nucleotide sequence