Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_81 and Pf6N2E2_82 are separated by 249 nucleotides Pf6N2E2_82 and Pf6N2E2_83 are separated by 361 nucleotides
Pf6N2E2_81 - hypothetical protein, at 126,543 to 126,749
_81
Pf6N2E2_82 - hypothetical protein, at 126,999 to 127,151
_82
Pf6N2E2_83 - transcriptional regulator, AraC family, at 127,513 to 128,382
_83
Position (kb)
126
127
128 Strain fitness (log2 ratio)
-2
-1
0
1 at 126.012 kb on + strand at 126.193 kb on + strand at 126.193 kb on + strand at 126.194 kb on - strand at 126.276 kb on - strand at 126.372 kb on - strand at 126.388 kb on - strand at 126.388 kb on - strand at 126.394 kb on - strand at 126.394 kb on - strand at 126.394 kb on - strand at 126.394 kb on - strand at 126.424 kb on + strand at 126.470 kb on + strand at 126.553 kb on - strand at 126.569 kb on - strand, within Pf6N2E2_81 at 126.569 kb on - strand, within Pf6N2E2_81 at 126.693 kb on - strand, within Pf6N2E2_81 at 126.747 kb on + strand at 126.748 kb on - strand at 126.748 kb on - strand at 126.815 kb on - strand at 126.815 kb on - strand at 126.815 kb on - strand at 126.881 kb on - strand at 126.964 kb on + strand at 126.964 kb on + strand at 126.964 kb on + strand at 126.964 kb on + strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.988 kb on + strand at 126.988 kb on + strand at 126.989 kb on - strand at 126.989 kb on - strand at 126.989 kb on - strand at 126.989 kb on - strand at 126.989 kb on - strand at 126.989 kb on - strand at 127.050 kb on - strand, within Pf6N2E2_82 at 127.093 kb on + strand, within Pf6N2E2_82 at 127.094 kb on - strand, within Pf6N2E2_82 at 127.094 kb on - strand, within Pf6N2E2_82 at 127.226 kb on + strand at 127.302 kb on - strand at 127.349 kb on - strand at 127.397 kb on + strand at 127.398 kb on - strand at 127.398 kb on - strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.529 kb on + strand at 127.529 kb on + strand at 127.529 kb on + strand at 127.529 kb on + strand at 127.529 kb on + strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.568 kb on + strand at 127.569 kb on - strand at 127.580 kb on + strand at 127.580 kb on + strand at 127.581 kb on - strand at 127.581 kb on - strand at 127.649 kb on + strand, within Pf6N2E2_83 at 127.890 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.987 kb on - strand, within Pf6N2E2_83 at 127.987 kb on - strand, within Pf6N2E2_83 at 127.987 kb on - strand, within Pf6N2E2_83 at 127.987 kb on - strand, within Pf6N2E2_83 at 128.140 kb on + strand, within Pf6N2E2_83
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 126,012 + -0.1 126,193 + +0.2 126,193 + +0.7 126,194 - +0.6 126,276 - +0.3 126,372 - -0.1 126,388 - +0.0 126,388 - -0.1 126,394 - +0.6 126,394 - +0.2 126,394 - -0.5 126,394 - +0.5 126,424 + +0.9 126,470 + -0.1 126,553 - -0.6 126,569 - Pf6N2E2_81 0.13 +0.0 126,569 - Pf6N2E2_81 0.13 -0.1 126,693 - Pf6N2E2_81 0.72 -0.3 126,747 + -0.4 126,748 - -1.9 126,748 - +0.0 126,815 - -0.3 126,815 - -0.0 126,815 - -0.3 126,881 - +0.3 126,964 + +0.3 126,964 + +0.5 126,964 + -0.3 126,964 + -0.1 126,965 - +0.4 126,965 - +0.9 126,965 - +0.6 126,965 - -2.3 126,965 - -0.3 126,965 - +0.5 126,965 - +0.5 126,965 - +0.5 126,965 - -0.7 126,988 + +0.4 126,988 + -0.2 126,989 - +0.9 126,989 - +0.9 126,989 - -0.2 126,989 - +0.1 126,989 - -0.1 126,989 - -0.9 127,050 - Pf6N2E2_82 0.33 -1.3 127,093 + Pf6N2E2_82 0.61 +0.1 127,094 - Pf6N2E2_82 0.62 -1.4 127,094 - Pf6N2E2_82 0.62 -1.6 127,226 + -0.1 127,302 - -0.3 127,349 - -0.5 127,397 + -0.5 127,398 - +0.5 127,398 - -0.4 127,412 + +0.9 127,412 + +0.3 127,412 + -0.6 127,413 - -0.7 127,413 - -0.0 127,413 - -0.7 127,413 - -0.2 127,413 - -0.4 127,413 - +0.5 127,529 + -0.1 127,529 + +0.8 127,529 + +0.5 127,529 + +1.1 127,529 + -0.4 127,530 - -0.4 127,530 - -0.1 127,530 - -0.4 127,530 - +0.7 127,530 - -2.5 127,530 - +0.1 127,530 - +0.8 127,530 - -1.0 127,530 - +0.8 127,568 + +0.7 127,569 - +0.2 127,580 + -0.6 127,580 + +0.2 127,581 - -0.6 127,581 - +0.6 127,649 + Pf6N2E2_83 0.16 +0.2 127,890 + Pf6N2E2_83 0.43 -0.3 127,986 + Pf6N2E2_83 0.54 +0.2 127,986 + Pf6N2E2_83 0.54 +1.1 127,986 + Pf6N2E2_83 0.54 -0.9 127,986 + Pf6N2E2_83 0.54 -0.1 127,986 + Pf6N2E2_83 0.54 +0.5 127,986 + Pf6N2E2_83 0.54 -0.6 127,987 - Pf6N2E2_83 0.54 +0.6 127,987 - Pf6N2E2_83 0.54 +1.1 127,987 - Pf6N2E2_83 0.54 +0.0 127,987 - Pf6N2E2_83 0.54 -0.7 128,140 + Pf6N2E2_83 0.72 +1.3
Or see this region's nucleotide sequence