Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6062 and Pf6N2E2_6063 are separated by 177 nucleotides Pf6N2E2_6063 and Pf6N2E2_6064 are separated by 42 nucleotides
Pf6N2E2_6062 - 2-methylcitrate synthase (EC 2.3.3.5) (from data) , at 6,881,787 to 6,882,914
_6062
Pf6N2E2_6063 - 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79), at 6,883,092 to 6,885,683
_6063
Pf6N2E2_6064 - 2-methylaconitate isomerase, at 6,885,726 to 6,886,919
_6064
Position (kb)
6883
6884
6885
6886 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 6882.193 kb on - strand, within Pf6N2E2_6062 at 6882.252 kb on - strand, within Pf6N2E2_6062 at 6882.389 kb on + strand, within Pf6N2E2_6062 at 6882.389 kb on + strand, within Pf6N2E2_6062 at 6882.390 kb on - strand, within Pf6N2E2_6062 at 6882.390 kb on - strand, within Pf6N2E2_6062 at 6882.576 kb on + strand, within Pf6N2E2_6062 at 6882.576 kb on + strand, within Pf6N2E2_6062 at 6882.576 kb on + strand, within Pf6N2E2_6062 at 6882.576 kb on + strand, within Pf6N2E2_6062 at 6882.576 kb on + strand, within Pf6N2E2_6062 at 6882.577 kb on - strand, within Pf6N2E2_6062 at 6882.578 kb on + strand, within Pf6N2E2_6062 at 6882.726 kb on + strand, within Pf6N2E2_6062 at 6882.727 kb on - strand, within Pf6N2E2_6062 at 6882.867 kb on + strand at 6882.868 kb on - strand at 6882.935 kb on - strand at 6882.935 kb on - strand at 6882.935 kb on - strand at 6883.137 kb on + strand at 6883.182 kb on + strand at 6883.183 kb on - strand at 6883.197 kb on + strand at 6883.984 kb on - strand, within Pf6N2E2_6063 at 6884.007 kb on + strand, within Pf6N2E2_6063 at 6884.007 kb on + strand, within Pf6N2E2_6063 at 6884.008 kb on - strand, within Pf6N2E2_6063 at 6884.031 kb on + strand, within Pf6N2E2_6063 at 6884.082 kb on + strand, within Pf6N2E2_6063 at 6884.130 kb on + strand, within Pf6N2E2_6063 at 6884.131 kb on - strand, within Pf6N2E2_6063 at 6884.439 kb on + strand, within Pf6N2E2_6063 at 6884.622 kb on - strand, within Pf6N2E2_6063 at 6884.631 kb on + strand, within Pf6N2E2_6063 at 6884.676 kb on + strand, within Pf6N2E2_6063 at 6884.676 kb on + strand, within Pf6N2E2_6063 at 6884.719 kb on + strand, within Pf6N2E2_6063 at 6884.720 kb on - strand, within Pf6N2E2_6063 at 6884.720 kb on - strand, within Pf6N2E2_6063 at 6884.832 kb on + strand, within Pf6N2E2_6063 at 6884.832 kb on + strand, within Pf6N2E2_6063 at 6884.832 kb on + strand, within Pf6N2E2_6063 at 6884.833 kb on - strand, within Pf6N2E2_6063 at 6884.833 kb on - strand, within Pf6N2E2_6063 at 6884.833 kb on - strand, within Pf6N2E2_6063 at 6884.833 kb on - strand, within Pf6N2E2_6063 at 6884.910 kb on + strand, within Pf6N2E2_6063 at 6884.911 kb on - strand, within Pf6N2E2_6063 at 6884.911 kb on - strand, within Pf6N2E2_6063 at 6884.911 kb on - strand, within Pf6N2E2_6063 at 6884.911 kb on - strand, within Pf6N2E2_6063 at 6885.060 kb on + strand, within Pf6N2E2_6063 at 6885.060 kb on + strand, within Pf6N2E2_6063 at 6885.060 kb on + strand, within Pf6N2E2_6063 at 6885.268 kb on - strand, within Pf6N2E2_6063 at 6885.268 kb on - strand, within Pf6N2E2_6063 at 6885.268 kb on - strand, within Pf6N2E2_6063 at 6885.268 kb on - strand, within Pf6N2E2_6063 at 6885.268 kb on - strand, within Pf6N2E2_6063 at 6885.495 kb on + strand at 6885.618 kb on + strand at 6885.618 kb on + strand at 6885.619 kb on - strand at 6885.619 kb on - strand at 6885.619 kb on - strand at 6885.681 kb on + strand at 6885.682 kb on - strand at 6885.759 kb on - strand at 6885.762 kb on + strand at 6885.762 kb on + strand at 6885.762 kb on + strand at 6885.762 kb on + strand at 6885.762 kb on + strand at 6885.762 kb on + strand at 6885.762 kb on + strand at 6885.762 kb on + strand at 6885.763 kb on - strand at 6885.763 kb on - strand at 6885.763 kb on - strand at 6885.763 kb on - strand at 6885.763 kb on - strand at 6885.763 kb on - strand at 6885.763 kb on - strand at 6885.763 kb on - strand at 6885.763 kb on - strand at 6885.763 kb on - strand at 6885.763 kb on - strand at 6885.764 kb on + strand at 6885.765 kb on - strand at 6885.765 kb on - strand at 6885.765 kb on - strand at 6885.885 kb on + strand, within Pf6N2E2_6064 at 6885.982 kb on - strand, within Pf6N2E2_6064 at 6885.982 kb on - strand, within Pf6N2E2_6064 at 6886.126 kb on + strand, within Pf6N2E2_6064 at 6886.587 kb on + strand, within Pf6N2E2_6064 at 6886.587 kb on + strand, within Pf6N2E2_6064 at 6886.588 kb on - strand, within Pf6N2E2_6064 at 6886.588 kb on - strand, within Pf6N2E2_6064 at 6886.588 kb on - strand, within Pf6N2E2_6064 at 6886.588 kb on - strand, within Pf6N2E2_6064 at 6886.633 kb on + strand, within Pf6N2E2_6064 at 6886.633 kb on + strand, within Pf6N2E2_6064 at 6886.634 kb on - strand, within Pf6N2E2_6064 at 6886.634 kb on - strand, within Pf6N2E2_6064 at 6886.634 kb on - strand, within Pf6N2E2_6064 at 6886.634 kb on - strand, within Pf6N2E2_6064
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 6,882,193 - Pf6N2E2_6062 0.36 -0.1 6,882,252 - Pf6N2E2_6062 0.41 -1.3 6,882,389 + Pf6N2E2_6062 0.53 -0.7 6,882,389 + Pf6N2E2_6062 0.53 -0.6 6,882,390 - Pf6N2E2_6062 0.53 +0.1 6,882,390 - Pf6N2E2_6062 0.53 +0.3 6,882,576 + Pf6N2E2_6062 0.70 -0.6 6,882,576 + Pf6N2E2_6062 0.70 -0.1 6,882,576 + Pf6N2E2_6062 0.70 -0.4 6,882,576 + Pf6N2E2_6062 0.70 -0.1 6,882,576 + Pf6N2E2_6062 0.70 +0.6 6,882,577 - Pf6N2E2_6062 0.70 +0.4 6,882,578 + Pf6N2E2_6062 0.70 -0.1 6,882,726 + Pf6N2E2_6062 0.83 +0.3 6,882,727 - Pf6N2E2_6062 0.83 +1.6 6,882,867 + +0.1 6,882,868 - +0.2 6,882,935 - -0.1 6,882,935 - +0.4 6,882,935 - -1.9 6,883,137 + -2.3 6,883,182 + -3.4 6,883,183 - -2.6 6,883,197 + -1.5 6,883,984 - Pf6N2E2_6063 0.34 -1.6 6,884,007 + Pf6N2E2_6063 0.35 -2.9 6,884,007 + Pf6N2E2_6063 0.35 -0.6 6,884,008 - Pf6N2E2_6063 0.35 -1.0 6,884,031 + Pf6N2E2_6063 0.36 -0.1 6,884,082 + Pf6N2E2_6063 0.38 -0.6 6,884,130 + Pf6N2E2_6063 0.40 -1.4 6,884,131 - Pf6N2E2_6063 0.40 -0.6 6,884,439 + Pf6N2E2_6063 0.52 -1.3 6,884,622 - Pf6N2E2_6063 0.59 -2.6 6,884,631 + Pf6N2E2_6063 0.59 -2.9 6,884,676 + Pf6N2E2_6063 0.61 -1.6 6,884,676 + Pf6N2E2_6063 0.61 -1.8 6,884,719 + Pf6N2E2_6063 0.63 -1.9 6,884,720 - Pf6N2E2_6063 0.63 -2.4 6,884,720 - Pf6N2E2_6063 0.63 -0.8 6,884,832 + Pf6N2E2_6063 0.67 -0.6 6,884,832 + Pf6N2E2_6063 0.67 -1.9 6,884,832 + Pf6N2E2_6063 0.67 +0.4 6,884,833 - Pf6N2E2_6063 0.67 -0.9 6,884,833 - Pf6N2E2_6063 0.67 +1.2 6,884,833 - Pf6N2E2_6063 0.67 -0.9 6,884,833 - Pf6N2E2_6063 0.67 -0.5 6,884,910 + Pf6N2E2_6063 0.70 -1.3 6,884,911 - Pf6N2E2_6063 0.70 -3.9 6,884,911 - Pf6N2E2_6063 0.70 -1.0 6,884,911 - Pf6N2E2_6063 0.70 -1.6 6,884,911 - Pf6N2E2_6063 0.70 -1.9 6,885,060 + Pf6N2E2_6063 0.76 -1.9 6,885,060 + Pf6N2E2_6063 0.76 -1.1 6,885,060 + Pf6N2E2_6063 0.76 -1.0 6,885,268 - Pf6N2E2_6063 0.84 -0.8 6,885,268 - Pf6N2E2_6063 0.84 -1.1 6,885,268 - Pf6N2E2_6063 0.84 -2.0 6,885,268 - Pf6N2E2_6063 0.84 -1.9 6,885,268 - Pf6N2E2_6063 0.84 -0.9 6,885,495 + -1.2 6,885,618 + -1.5 6,885,618 + -1.5 6,885,619 - -1.7 6,885,619 - -2.9 6,885,619 - -0.3 6,885,681 + +1.2 6,885,682 - -0.6 6,885,759 - -1.7 6,885,762 + -1.4 6,885,762 + -0.8 6,885,762 + -2.8 6,885,762 + -0.5 6,885,762 + -0.9 6,885,762 + -1.4 6,885,762 + -2.6 6,885,762 + -2.2 6,885,763 - -2.6 6,885,763 - -0.3 6,885,763 - -2.4 6,885,763 - -0.7 6,885,763 - -2.5 6,885,763 - -1.7 6,885,763 - -1.2 6,885,763 - -0.7 6,885,763 - -1.4 6,885,763 - -1.2 6,885,763 - -2.4 6,885,764 + -0.5 6,885,765 - -3.7 6,885,765 - -1.3 6,885,765 - -1.1 6,885,885 + Pf6N2E2_6064 0.13 -0.9 6,885,982 - Pf6N2E2_6064 0.21 -0.6 6,885,982 - Pf6N2E2_6064 0.21 -1.7 6,886,126 + Pf6N2E2_6064 0.34 -3.1 6,886,587 + Pf6N2E2_6064 0.72 -0.1 6,886,587 + Pf6N2E2_6064 0.72 -0.8 6,886,588 - Pf6N2E2_6064 0.72 -1.3 6,886,588 - Pf6N2E2_6064 0.72 -0.1 6,886,588 - Pf6N2E2_6064 0.72 -2.2 6,886,588 - Pf6N2E2_6064 0.72 -1.5 6,886,633 + Pf6N2E2_6064 0.76 -1.7 6,886,633 + Pf6N2E2_6064 0.76 -1.0 6,886,634 - Pf6N2E2_6064 0.76 -1.2 6,886,634 - Pf6N2E2_6064 0.76 -2.0 6,886,634 - Pf6N2E2_6064 0.76 -2.4 6,886,634 - Pf6N2E2_6064 0.76 -1.0
Or see this region's nucleotide sequence