Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_571 and Pf6N2E2_572 are separated by 184 nucleotides Pf6N2E2_572 and Pf6N2E2_573 are separated by 67 nucleotides Pf6N2E2_573 and Pf6N2E2_574 are separated by 143 nucleotides Pf6N2E2_574 and Pf6N2E2_575 are separated by 218 nucleotides
Pf6N2E2_571 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 645,326 to 646,369
_571
Pf6N2E2_572 - hypothetical protein, at 646,554 to 647,033
_572
Pf6N2E2_573 - Potassium uptake protein TrkH, at 647,101 to 648,555
_573
Pf6N2E2_574 - Transporter, LysE family, at 648,699 to 649,286
_574
Pf6N2E2_575 - Transcriptional regulator, AraC family, at 649,505 to 650,329
_575
Position (kb) 647
648
649 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 646.298 kb on - strand at 646.400 kb on + strand at 646.400 kb on + strand at 646.420 kb on + strand at 646.420 kb on + strand at 646.420 kb on + strand at 646.420 kb on + strand at 646.421 kb on - strand at 646.421 kb on - strand at 646.517 kb on - strand at 646.565 kb on + strand at 646.565 kb on + strand at 646.566 kb on - strand at 646.656 kb on + strand, within Pf6N2E2_572 at 646.656 kb on + strand, within Pf6N2E2_572 at 646.926 kb on - strand, within Pf6N2E2_572 at 646.973 kb on + strand, within Pf6N2E2_572 at 647.106 kb on - strand at 647.174 kb on - strand at 647.347 kb on - strand, within Pf6N2E2_573 at 647.347 kb on - strand, within Pf6N2E2_573 at 647.412 kb on - strand, within Pf6N2E2_573 at 647.467 kb on + strand, within Pf6N2E2_573 at 647.468 kb on - strand, within Pf6N2E2_573 at 647.480 kb on - strand, within Pf6N2E2_573 at 647.480 kb on - strand, within Pf6N2E2_573 at 647.546 kb on + strand, within Pf6N2E2_573 at 647.547 kb on - strand, within Pf6N2E2_573 at 647.547 kb on - strand, within Pf6N2E2_573 at 647.547 kb on - strand, within Pf6N2E2_573 at 647.579 kb on - strand, within Pf6N2E2_573 at 647.585 kb on + strand, within Pf6N2E2_573 at 647.585 kb on + strand, within Pf6N2E2_573 at 647.585 kb on + strand, within Pf6N2E2_573 at 647.586 kb on - strand, within Pf6N2E2_573 at 647.586 kb on - strand, within Pf6N2E2_573 at 647.790 kb on - strand, within Pf6N2E2_573 at 647.990 kb on + strand, within Pf6N2E2_573 at 647.990 kb on + strand, within Pf6N2E2_573 at 647.990 kb on + strand, within Pf6N2E2_573 at 647.990 kb on + strand, within Pf6N2E2_573 at 647.991 kb on - strand, within Pf6N2E2_573 at 647.991 kb on - strand, within Pf6N2E2_573 at 648.005 kb on + strand, within Pf6N2E2_573 at 648.005 kb on + strand, within Pf6N2E2_573 at 648.005 kb on + strand, within Pf6N2E2_573 at 648.005 kb on + strand, within Pf6N2E2_573 at 648.006 kb on - strand, within Pf6N2E2_573 at 648.006 kb on - strand, within Pf6N2E2_573 at 648.006 kb on - strand, within Pf6N2E2_573 at 648.006 kb on - strand, within Pf6N2E2_573 at 648.006 kb on - strand, within Pf6N2E2_573 at 648.006 kb on - strand, within Pf6N2E2_573 at 648.257 kb on + strand, within Pf6N2E2_573 at 648.257 kb on + strand, within Pf6N2E2_573 at 648.335 kb on + strand, within Pf6N2E2_573 at 648.335 kb on + strand, within Pf6N2E2_573 at 648.335 kb on + strand, within Pf6N2E2_573 at 648.335 kb on + strand, within Pf6N2E2_573 at 648.335 kb on + strand, within Pf6N2E2_573 at 648.335 kb on + strand, within Pf6N2E2_573 at 648.336 kb on - strand, within Pf6N2E2_573 at 648.336 kb on - strand, within Pf6N2E2_573 at 648.336 kb on - strand, within Pf6N2E2_573 at 648.336 kb on - strand, within Pf6N2E2_573 at 648.336 kb on - strand, within Pf6N2E2_573 at 648.336 kb on - strand, within Pf6N2E2_573 at 648.336 kb on - strand, within Pf6N2E2_573 at 648.336 kb on - strand, within Pf6N2E2_573 at 648.336 kb on - strand, within Pf6N2E2_573 at 648.336 kb on - strand, within Pf6N2E2_573 at 648.336 kb on - strand, within Pf6N2E2_573 at 648.336 kb on - strand, within Pf6N2E2_573 at 648.336 kb on - strand, within Pf6N2E2_573 at 648.336 kb on - strand, within Pf6N2E2_573 at 648.336 kb on - strand, within Pf6N2E2_573 at 648.455 kb on + strand at 648.455 kb on + strand at 648.455 kb on + strand at 648.455 kb on + strand at 648.455 kb on + strand at 648.455 kb on + strand at 648.455 kb on + strand at 648.455 kb on + strand at 648.456 kb on - strand at 648.456 kb on - strand at 648.456 kb on - strand at 648.555 kb on + strand at 648.555 kb on + strand at 648.555 kb on + strand at 648.555 kb on + strand at 648.555 kb on + strand at 648.555 kb on + strand at 648.555 kb on + strand at 648.556 kb on - strand at 648.556 kb on - strand at 648.556 kb on - strand at 648.556 kb on - strand at 648.587 kb on - strand at 648.659 kb on + strand at 648.659 kb on + strand at 648.682 kb on + strand at 648.682 kb on + strand at 648.683 kb on - strand at 648.683 kb on - strand at 648.683 kb on - strand at 648.693 kb on + strand at 648.746 kb on + strand at 648.746 kb on + strand at 648.747 kb on - strand at 648.747 kb on - strand at 649.020 kb on + strand, within Pf6N2E2_574 at 649.020 kb on + strand, within Pf6N2E2_574 at 649.134 kb on + strand, within Pf6N2E2_574 at 649.135 kb on - strand, within Pf6N2E2_574 at 649.135 kb on - strand, within Pf6N2E2_574 at 649.149 kb on + strand, within Pf6N2E2_574 at 649.205 kb on + strand, within Pf6N2E2_574 at 649.269 kb on + strand at 649.269 kb on + strand at 649.273 kb on + strand at 649.273 kb on + strand at 649.273 kb on + strand at 649.273 kb on + strand at 649.326 kb on + strand at 649.326 kb on + strand at 649.326 kb on + strand at 649.326 kb on + strand at 649.327 kb on - strand at 649.327 kb on - strand at 649.337 kb on + strand at 649.504 kb on - strand at 649.516 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 646,298 - -0.2 646,400 + -0.7 646,400 + +1.1 646,420 + -0.1 646,420 + +1.0 646,420 + +0.3 646,420 + +0.4 646,421 - -0.3 646,421 - -0.6 646,517 - -0.2 646,565 + -0.6 646,565 + +0.5 646,566 - -3.0 646,656 + Pf6N2E2_572 0.21 -0.9 646,656 + Pf6N2E2_572 0.21 +0.1 646,926 - Pf6N2E2_572 0.78 -0.2 646,973 + Pf6N2E2_572 0.87 -0.2 647,106 - +0.1 647,174 - +0.1 647,347 - Pf6N2E2_573 0.17 +0.5 647,347 - Pf6N2E2_573 0.17 +0.2 647,412 - Pf6N2E2_573 0.21 +0.6 647,467 + Pf6N2E2_573 0.25 +0.3 647,468 - Pf6N2E2_573 0.25 -0.8 647,480 - Pf6N2E2_573 0.26 +0.1 647,480 - Pf6N2E2_573 0.26 +0.1 647,546 + Pf6N2E2_573 0.31 -0.6 647,547 - Pf6N2E2_573 0.31 +0.5 647,547 - Pf6N2E2_573 0.31 -0.9 647,547 - Pf6N2E2_573 0.31 -1.3 647,579 - Pf6N2E2_573 0.33 -1.8 647,585 + Pf6N2E2_573 0.33 -0.7 647,585 + Pf6N2E2_573 0.33 +0.7 647,585 + Pf6N2E2_573 0.33 -0.2 647,586 - Pf6N2E2_573 0.33 -1.3 647,586 - Pf6N2E2_573 0.33 -0.7 647,790 - Pf6N2E2_573 0.47 +0.5 647,990 + Pf6N2E2_573 0.61 +0.1 647,990 + Pf6N2E2_573 0.61 +0.1 647,990 + Pf6N2E2_573 0.61 +0.4 647,990 + Pf6N2E2_573 0.61 -0.5 647,991 - Pf6N2E2_573 0.61 +0.6 647,991 - Pf6N2E2_573 0.61 +0.0 648,005 + Pf6N2E2_573 0.62 -0.1 648,005 + Pf6N2E2_573 0.62 -0.4 648,005 + Pf6N2E2_573 0.62 -0.3 648,005 + Pf6N2E2_573 0.62 +0.8 648,006 - Pf6N2E2_573 0.62 -0.3 648,006 - Pf6N2E2_573 0.62 -0.2 648,006 - Pf6N2E2_573 0.62 -0.3 648,006 - Pf6N2E2_573 0.62 +0.8 648,006 - Pf6N2E2_573 0.62 +1.0 648,006 - Pf6N2E2_573 0.62 -1.3 648,257 + Pf6N2E2_573 0.79 -0.3 648,257 + Pf6N2E2_573 0.79 -0.1 648,335 + Pf6N2E2_573 0.85 -1.1 648,335 + Pf6N2E2_573 0.85 +0.9 648,335 + Pf6N2E2_573 0.85 +0.4 648,335 + Pf6N2E2_573 0.85 -1.2 648,335 + Pf6N2E2_573 0.85 +0.3 648,335 + Pf6N2E2_573 0.85 +1.1 648,336 - Pf6N2E2_573 0.85 -0.0 648,336 - Pf6N2E2_573 0.85 -0.6 648,336 - Pf6N2E2_573 0.85 -0.8 648,336 - Pf6N2E2_573 0.85 -0.3 648,336 - Pf6N2E2_573 0.85 +1.3 648,336 - Pf6N2E2_573 0.85 -0.3 648,336 - Pf6N2E2_573 0.85 +0.4 648,336 - Pf6N2E2_573 0.85 +1.1 648,336 - Pf6N2E2_573 0.85 -1.0 648,336 - Pf6N2E2_573 0.85 +0.2 648,336 - Pf6N2E2_573 0.85 +0.1 648,336 - Pf6N2E2_573 0.85 -0.0 648,336 - Pf6N2E2_573 0.85 +0.1 648,336 - Pf6N2E2_573 0.85 +0.3 648,336 - Pf6N2E2_573 0.85 -0.7 648,455 + +0.6 648,455 + -0.7 648,455 + +0.5 648,455 + -0.3 648,455 + +0.7 648,455 + +0.7 648,455 + +0.5 648,455 + +1.9 648,456 - +0.5 648,456 - -0.0 648,456 - -0.1 648,555 + +0.2 648,555 + +0.4 648,555 + +0.1 648,555 + +2.3 648,555 + +0.1 648,555 + -0.6 648,555 + -0.2 648,556 - +0.8 648,556 - -0.0 648,556 - -0.2 648,556 - +0.1 648,587 - +0.0 648,659 + -0.8 648,659 + +0.2 648,682 + -1.5 648,682 + +0.2 648,683 - -0.4 648,683 - -0.2 648,683 - +0.1 648,693 + -0.3 648,746 + +0.5 648,746 + -0.4 648,747 - +0.3 648,747 - +0.1 649,020 + Pf6N2E2_574 0.55 -0.5 649,020 + Pf6N2E2_574 0.55 -1.1 649,134 + Pf6N2E2_574 0.74 -2.1 649,135 - Pf6N2E2_574 0.74 +0.1 649,135 - Pf6N2E2_574 0.74 -2.0 649,149 + Pf6N2E2_574 0.77 +1.2 649,205 + Pf6N2E2_574 0.86 +0.4 649,269 + -2.2 649,269 + -0.5 649,273 + +0.2 649,273 + +1.5 649,273 + +0.2 649,273 + -0.2 649,326 + -0.9 649,326 + +0.5 649,326 + +0.8 649,326 + +0.4 649,327 - +0.6 649,327 - -0.2 649,337 + +0.8 649,504 - +0.7 649,516 - -1.6
Or see this region's nucleotide sequence