Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5480 and Pf6N2E2_5481 are separated by 25 nucleotides Pf6N2E2_5481 and Pf6N2E2_5482 are separated by 328 nucleotides Pf6N2E2_5482 and Pf6N2E2_5483 are separated by 7 nucleotides
Pf6N2E2_5480 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 6,298,058 to 6,299,113
_5480
Pf6N2E2_5481 - Transcriptional regulator, AraC family, at 6,299,139 to 6,300,068
_5481
Pf6N2E2_5482 - FIG00954888: hypothetical protein, at 6,300,397 to 6,300,750
_5482
Pf6N2E2_5483 - hypothetical protein, at 6,300,758 to 6,301,027
_5483
Position (kb)
6299
6300
6301 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.722 kb on - strand, within Pf6N2E2_5480 at 6299.026 kb on + strand at 6299.026 kb on + strand at 6299.027 kb on - strand at 6299.027 kb on - strand at 6299.080 kb on - strand at 6299.200 kb on + strand at 6299.317 kb on + strand, within Pf6N2E2_5481 at 6299.317 kb on + strand, within Pf6N2E2_5481 at 6299.317 kb on + strand, within Pf6N2E2_5481 at 6299.528 kb on - strand, within Pf6N2E2_5481 at 6299.580 kb on - strand, within Pf6N2E2_5481 at 6299.641 kb on + strand, within Pf6N2E2_5481 at 6299.641 kb on + strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.671 kb on - strand, within Pf6N2E2_5481 at 6299.809 kb on + strand, within Pf6N2E2_5481 at 6299.824 kb on + strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.912 kb on + strand, within Pf6N2E2_5481 at 6299.913 kb on - strand, within Pf6N2E2_5481 at 6299.913 kb on - strand, within Pf6N2E2_5481 at 6299.962 kb on + strand, within Pf6N2E2_5481 at 6299.963 kb on - strand, within Pf6N2E2_5481 at 6299.963 kb on - strand, within Pf6N2E2_5481 at 6299.963 kb on - strand, within Pf6N2E2_5481 at 6300.028 kb on + strand at 6300.029 kb on - strand at 6300.029 kb on - strand at 6300.109 kb on + strand at 6300.109 kb on + strand at 6300.109 kb on + strand at 6300.110 kb on - strand at 6300.110 kb on - strand at 6300.110 kb on - strand at 6300.133 kb on - strand at 6300.263 kb on + strand at 6300.263 kb on + strand at 6300.264 kb on - strand at 6300.264 kb on - strand at 6300.334 kb on + strand at 6300.334 kb on + strand at 6300.402 kb on + strand at 6300.402 kb on + strand at 6300.402 kb on + strand at 6300.403 kb on - strand at 6300.403 kb on - strand at 6300.403 kb on - strand at 6300.480 kb on + strand, within Pf6N2E2_5482 at 6300.481 kb on - strand, within Pf6N2E2_5482 at 6300.604 kb on + strand, within Pf6N2E2_5482 at 6300.605 kb on - strand, within Pf6N2E2_5482 at 6300.605 kb on - strand, within Pf6N2E2_5482 at 6300.605 kb on - strand, within Pf6N2E2_5482 at 6300.605 kb on - strand, within Pf6N2E2_5482 at 6300.629 kb on + strand, within Pf6N2E2_5482 at 6300.630 kb on - strand, within Pf6N2E2_5482 at 6300.630 kb on - strand, within Pf6N2E2_5482 at 6300.678 kb on + strand, within Pf6N2E2_5482 at 6300.678 kb on + strand, within Pf6N2E2_5482 at 6300.678 kb on + strand, within Pf6N2E2_5482 at 6300.679 kb on - strand, within Pf6N2E2_5482 at 6300.679 kb on - strand, within Pf6N2E2_5482 at 6300.731 kb on + strand at 6300.802 kb on + strand, within Pf6N2E2_5483 at 6300.802 kb on + strand, within Pf6N2E2_5483 at 6300.803 kb on - strand, within Pf6N2E2_5483 at 6300.803 kb on - strand, within Pf6N2E2_5483 at 6300.803 kb on - strand, within Pf6N2E2_5483 at 6300.837 kb on + strand, within Pf6N2E2_5483 at 6300.838 kb on - strand, within Pf6N2E2_5483
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 6,298,648 + Pf6N2E2_5480 0.56 +0.1 6,298,648 + Pf6N2E2_5480 0.56 +0.1 6,298,648 + Pf6N2E2_5480 0.56 -1.3 6,298,648 + Pf6N2E2_5480 0.56 +0.3 6,298,648 + Pf6N2E2_5480 0.56 -0.1 6,298,649 - Pf6N2E2_5480 0.56 +0.4 6,298,649 - Pf6N2E2_5480 0.56 +0.5 6,298,649 - Pf6N2E2_5480 0.56 +1.1 6,298,649 - Pf6N2E2_5480 0.56 -0.0 6,298,649 - Pf6N2E2_5480 0.56 -0.2 6,298,722 - Pf6N2E2_5480 0.63 -0.1 6,299,026 + +0.2 6,299,026 + -0.0 6,299,027 - +0.2 6,299,027 - +0.7 6,299,080 - +0.3 6,299,200 + +3.9 6,299,317 + Pf6N2E2_5481 0.19 +3.9 6,299,317 + Pf6N2E2_5481 0.19 +3.9 6,299,317 + Pf6N2E2_5481 0.19 +4.3 6,299,528 - Pf6N2E2_5481 0.42 +4.4 6,299,580 - Pf6N2E2_5481 0.47 +4.1 6,299,641 + Pf6N2E2_5481 0.54 +4.2 6,299,641 + Pf6N2E2_5481 0.54 +3.5 6,299,642 - Pf6N2E2_5481 0.54 +3.9 6,299,642 - Pf6N2E2_5481 0.54 +4.7 6,299,642 - Pf6N2E2_5481 0.54 +4.4 6,299,642 - Pf6N2E2_5481 0.54 +4.0 6,299,671 - Pf6N2E2_5481 0.57 +4.2 6,299,809 + Pf6N2E2_5481 0.72 +3.8 6,299,824 + Pf6N2E2_5481 0.74 +4.6 6,299,825 - Pf6N2E2_5481 0.74 +3.8 6,299,825 - Pf6N2E2_5481 0.74 +4.2 6,299,825 - Pf6N2E2_5481 0.74 +3.8 6,299,825 - Pf6N2E2_5481 0.74 +4.2 6,299,912 + Pf6N2E2_5481 0.83 +4.4 6,299,913 - Pf6N2E2_5481 0.83 +3.8 6,299,913 - Pf6N2E2_5481 0.83 +4.6 6,299,962 + Pf6N2E2_5481 0.88 +3.6 6,299,963 - Pf6N2E2_5481 0.89 +4.0 6,299,963 - Pf6N2E2_5481 0.89 +4.5 6,299,963 - Pf6N2E2_5481 0.89 +4.4 6,300,028 + +3.9 6,300,029 - +3.9 6,300,029 - +4.0 6,300,109 + +3.5 6,300,109 + +3.9 6,300,109 + +3.7 6,300,110 - +0.7 6,300,110 - +1.0 6,300,110 - +2.3 6,300,133 - +1.5 6,300,263 + -1.8 6,300,263 + -0.3 6,300,264 - -1.0 6,300,264 - -2.2 6,300,334 + +4.8 6,300,334 + +4.6 6,300,402 + +4.9 6,300,402 + +4.2 6,300,402 + +4.1 6,300,403 - +4.1 6,300,403 - +4.7 6,300,403 - +4.4 6,300,480 + Pf6N2E2_5482 0.23 +4.3 6,300,481 - Pf6N2E2_5482 0.24 +4.2 6,300,604 + Pf6N2E2_5482 0.58 +3.6 6,300,605 - Pf6N2E2_5482 0.59 +4.3 6,300,605 - Pf6N2E2_5482 0.59 +4.4 6,300,605 - Pf6N2E2_5482 0.59 +4.1 6,300,605 - Pf6N2E2_5482 0.59 +3.9 6,300,629 + Pf6N2E2_5482 0.66 +4.6 6,300,630 - Pf6N2E2_5482 0.66 +4.2 6,300,630 - Pf6N2E2_5482 0.66 +4.0 6,300,678 + Pf6N2E2_5482 0.79 +3.8 6,300,678 + Pf6N2E2_5482 0.79 +3.2 6,300,678 + Pf6N2E2_5482 0.79 +4.0 6,300,679 - Pf6N2E2_5482 0.80 +3.4 6,300,679 - Pf6N2E2_5482 0.80 +4.3 6,300,731 + +1.5 6,300,802 + Pf6N2E2_5483 0.16 +2.2 6,300,802 + Pf6N2E2_5483 0.16 +2.4 6,300,803 - Pf6N2E2_5483 0.17 +1.6 6,300,803 - Pf6N2E2_5483 0.17 +1.3 6,300,803 - Pf6N2E2_5483 0.17 +2.2 6,300,837 + Pf6N2E2_5483 0.29 +2.5 6,300,838 - Pf6N2E2_5483 0.30 +1.1
Or see this region's nucleotide sequence