Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5318 and Pf6N2E2_5319 are separated by 3 nucleotides Pf6N2E2_5319 and Pf6N2E2_5320 are separated by 0 nucleotides Pf6N2E2_5320 and Pf6N2E2_5321 are separated by 11 nucleotides
Pf6N2E2_5318 - Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-), at 6,113,325 to 6,115,355
_5318
Pf6N2E2_5319 - Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-), at 6,115,359 to 6,115,985
_5319
Pf6N2E2_5320 - Cytochrome O ubiquinol oxidase subunit IV (EC 1.10.3.-), at 6,115,986 to 6,116,321
_5320
Pf6N2E2_5321 - Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB, at 6,116,333 to 6,117,220
_5321
Position (kb)
6115
6116
6117 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6115.068 kb on + strand, within Pf6N2E2_5318 at 6115.068 kb on + strand, within Pf6N2E2_5318 at 6115.068 kb on + strand, within Pf6N2E2_5318 at 6115.068 kb on + strand, within Pf6N2E2_5318 at 6115.068 kb on + strand, within Pf6N2E2_5318 at 6115.069 kb on - strand, within Pf6N2E2_5318 at 6115.069 kb on - strand, within Pf6N2E2_5318 at 6115.069 kb on - strand, within Pf6N2E2_5318 at 6115.069 kb on - strand, within Pf6N2E2_5318 at 6115.069 kb on - strand, within Pf6N2E2_5318 at 6115.069 kb on - strand, within Pf6N2E2_5318 at 6115.200 kb on + strand at 6115.201 kb on - strand at 6115.236 kb on + strand at 6115.236 kb on + strand at 6115.237 kb on - strand at 6115.237 kb on - strand at 6115.353 kb on + strand at 6115.353 kb on + strand at 6115.353 kb on + strand at 6115.354 kb on - strand at 6115.369 kb on + strand at 6115.369 kb on + strand at 6115.370 kb on - strand at 6115.370 kb on - strand at 6115.464 kb on + strand, within Pf6N2E2_5319 at 6115.465 kb on - strand, within Pf6N2E2_5319 at 6115.509 kb on + strand, within Pf6N2E2_5319 at 6115.524 kb on - strand, within Pf6N2E2_5319 at 6115.572 kb on + strand, within Pf6N2E2_5319 at 6115.573 kb on - strand, within Pf6N2E2_5319 at 6115.576 kb on + strand, within Pf6N2E2_5319 at 6115.576 kb on + strand, within Pf6N2E2_5319 at 6115.576 kb on + strand, within Pf6N2E2_5319 at 6115.577 kb on - strand, within Pf6N2E2_5319 at 6115.577 kb on - strand, within Pf6N2E2_5319 at 6115.617 kb on + strand, within Pf6N2E2_5319 at 6115.617 kb on + strand, within Pf6N2E2_5319 at 6115.618 kb on - strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.855 kb on - strand, within Pf6N2E2_5319 at 6115.855 kb on - strand, within Pf6N2E2_5319 at 6115.855 kb on - strand, within Pf6N2E2_5319 at 6115.855 kb on - strand, within Pf6N2E2_5319 at 6115.855 kb on - strand, within Pf6N2E2_5319 at 6115.965 kb on + strand at 6115.965 kb on + strand at 6115.965 kb on + strand at 6115.966 kb on - strand at 6115.966 kb on - strand at 6115.983 kb on + strand at 6115.983 kb on + strand at 6115.983 kb on + strand at 6115.983 kb on + strand at 6115.984 kb on - strand at 6115.984 kb on - strand at 6115.984 kb on - strand at 6116.044 kb on - strand, within Pf6N2E2_5320 at 6116.103 kb on + strand, within Pf6N2E2_5320 at 6116.103 kb on + strand, within Pf6N2E2_5320 at 6116.104 kb on - strand, within Pf6N2E2_5320 at 6116.104 kb on - strand, within Pf6N2E2_5320 at 6116.164 kb on + strand, within Pf6N2E2_5320 at 6116.164 kb on + strand, within Pf6N2E2_5320 at 6116.164 kb on + strand, within Pf6N2E2_5320 at 6116.165 kb on - strand, within Pf6N2E2_5320 at 6116.181 kb on + strand, within Pf6N2E2_5320 at 6116.181 kb on + strand, within Pf6N2E2_5320 at 6116.181 kb on + strand, within Pf6N2E2_5320 at 6116.182 kb on - strand, within Pf6N2E2_5320 at 6116.182 kb on - strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.636 kb on + strand, within Pf6N2E2_5321 at 6116.691 kb on - strand, within Pf6N2E2_5321 at 6116.691 kb on - strand, within Pf6N2E2_5321 at 6116.691 kb on - strand, within Pf6N2E2_5321 at 6116.703 kb on - strand, within Pf6N2E2_5321 at 6116.703 kb on - strand, within Pf6N2E2_5321 at 6116.711 kb on + strand, within Pf6N2E2_5321 at 6116.784 kb on - strand, within Pf6N2E2_5321 at 6116.864 kb on + strand, within Pf6N2E2_5321 at 6116.865 kb on - strand, within Pf6N2E2_5321 at 6116.865 kb on - strand, within Pf6N2E2_5321 at 6116.865 kb on - strand, within Pf6N2E2_5321 at 6116.966 kb on + strand, within Pf6N2E2_5321 at 6116.966 kb on + strand, within Pf6N2E2_5321 at 6116.967 kb on - strand, within Pf6N2E2_5321 at 6116.967 kb on - strand, within Pf6N2E2_5321 at 6117.029 kb on + strand, within Pf6N2E2_5321 at 6117.029 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.060 kb on - strand, within Pf6N2E2_5321 at 6117.060 kb on - strand, within Pf6N2E2_5321 at 6117.060 kb on - strand, within Pf6N2E2_5321 at 6117.060 kb on - strand, within Pf6N2E2_5321 at 6117.060 kb on - strand, within Pf6N2E2_5321 at 6117.068 kb on + strand, within Pf6N2E2_5321 at 6117.068 kb on + strand, within Pf6N2E2_5321 at 6117.068 kb on + strand, within Pf6N2E2_5321 at 6117.068 kb on + strand, within Pf6N2E2_5321 at 6117.086 kb on + strand, within Pf6N2E2_5321 at 6117.209 kb on + strand at 6117.209 kb on + strand at 6117.209 kb on + strand at 6117.209 kb on + strand at 6117.210 kb on - strand at 6117.210 kb on - strand at 6117.218 kb on + strand at 6117.218 kb on + strand at 6117.218 kb on + strand at 6117.219 kb on - strand at 6117.219 kb on - strand at 6117.219 kb on - strand at 6117.219 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 6,115,068 + Pf6N2E2_5318 0.86 +0.3 6,115,068 + Pf6N2E2_5318 0.86 -1.0 6,115,068 + Pf6N2E2_5318 0.86 -1.6 6,115,068 + Pf6N2E2_5318 0.86 -0.9 6,115,068 + Pf6N2E2_5318 0.86 -3.4 6,115,069 - Pf6N2E2_5318 0.86 -0.3 6,115,069 - Pf6N2E2_5318 0.86 -0.3 6,115,069 - Pf6N2E2_5318 0.86 -2.1 6,115,069 - Pf6N2E2_5318 0.86 +0.3 6,115,069 - Pf6N2E2_5318 0.86 -0.3 6,115,069 - Pf6N2E2_5318 0.86 -1.0 6,115,200 + -1.0 6,115,201 - -1.9 6,115,236 + -2.6 6,115,236 + -1.9 6,115,237 - -0.2 6,115,237 - -0.8 6,115,353 + -1.5 6,115,353 + -1.0 6,115,353 + -1.2 6,115,354 - -1.7 6,115,369 + -3.5 6,115,369 + -2.7 6,115,370 - -2.0 6,115,370 - -2.6 6,115,464 + Pf6N2E2_5319 0.17 -1.0 6,115,465 - Pf6N2E2_5319 0.17 +0.1 6,115,509 + Pf6N2E2_5319 0.24 -1.4 6,115,524 - Pf6N2E2_5319 0.26 +0.2 6,115,572 + Pf6N2E2_5319 0.34 +0.0 6,115,573 - Pf6N2E2_5319 0.34 -1.8 6,115,576 + Pf6N2E2_5319 0.35 +0.2 6,115,576 + Pf6N2E2_5319 0.35 -3.1 6,115,576 + Pf6N2E2_5319 0.35 -3.8 6,115,577 - Pf6N2E2_5319 0.35 -1.4 6,115,577 - Pf6N2E2_5319 0.35 -0.3 6,115,617 + Pf6N2E2_5319 0.41 -1.7 6,115,617 + Pf6N2E2_5319 0.41 -1.0 6,115,618 - Pf6N2E2_5319 0.41 -1.1 6,115,854 + Pf6N2E2_5319 0.79 -3.0 6,115,854 + Pf6N2E2_5319 0.79 -1.3 6,115,854 + Pf6N2E2_5319 0.79 -0.9 6,115,854 + Pf6N2E2_5319 0.79 -3.2 6,115,854 + Pf6N2E2_5319 0.79 -0.9 6,115,854 + Pf6N2E2_5319 0.79 -0.5 6,115,854 + Pf6N2E2_5319 0.79 -0.7 6,115,854 + Pf6N2E2_5319 0.79 -2.4 6,115,855 - Pf6N2E2_5319 0.79 -1.9 6,115,855 - Pf6N2E2_5319 0.79 -0.0 6,115,855 - Pf6N2E2_5319 0.79 -1.8 6,115,855 - Pf6N2E2_5319 0.79 -2.3 6,115,855 - Pf6N2E2_5319 0.79 -2.7 6,115,965 + -1.0 6,115,965 + -0.2 6,115,965 + -0.7 6,115,966 - -0.4 6,115,966 - -3.1 6,115,983 + -2.5 6,115,983 + -2.3 6,115,983 + -1.4 6,115,983 + -1.0 6,115,984 - -0.1 6,115,984 - +0.2 6,115,984 - -2.7 6,116,044 - Pf6N2E2_5320 0.17 -0.0 6,116,103 + Pf6N2E2_5320 0.35 -0.6 6,116,103 + Pf6N2E2_5320 0.35 -2.2 6,116,104 - Pf6N2E2_5320 0.35 -1.7 6,116,104 - Pf6N2E2_5320 0.35 -2.4 6,116,164 + Pf6N2E2_5320 0.53 -1.7 6,116,164 + Pf6N2E2_5320 0.53 -1.2 6,116,164 + Pf6N2E2_5320 0.53 -0.9 6,116,165 - Pf6N2E2_5320 0.53 -0.7 6,116,181 + Pf6N2E2_5320 0.58 -1.7 6,116,181 + Pf6N2E2_5320 0.58 -2.0 6,116,181 + Pf6N2E2_5320 0.58 -0.7 6,116,182 - Pf6N2E2_5320 0.58 -2.3 6,116,182 - Pf6N2E2_5320 0.58 -1.4 6,116,216 + Pf6N2E2_5320 0.68 -2.3 6,116,216 + Pf6N2E2_5320 0.68 -2.4 6,116,216 + Pf6N2E2_5320 0.68 -1.3 6,116,216 + Pf6N2E2_5320 0.68 -2.6 6,116,216 + Pf6N2E2_5320 0.68 -1.1 6,116,216 + Pf6N2E2_5320 0.68 -0.6 6,116,216 + Pf6N2E2_5320 0.68 +0.3 6,116,217 - Pf6N2E2_5320 0.69 -2.2 6,116,217 - Pf6N2E2_5320 0.69 -1.1 6,116,217 - Pf6N2E2_5320 0.69 -1.5 6,116,217 - Pf6N2E2_5320 0.69 -1.3 6,116,217 - Pf6N2E2_5320 0.69 -1.0 6,116,217 - Pf6N2E2_5320 0.69 -1.2 6,116,636 + Pf6N2E2_5321 0.34 -1.1 6,116,691 - Pf6N2E2_5321 0.40 -1.4 6,116,691 - Pf6N2E2_5321 0.40 -0.0 6,116,691 - Pf6N2E2_5321 0.40 -1.6 6,116,703 - Pf6N2E2_5321 0.42 -2.3 6,116,703 - Pf6N2E2_5321 0.42 -2.4 6,116,711 + Pf6N2E2_5321 0.43 -1.2 6,116,784 - Pf6N2E2_5321 0.51 -1.1 6,116,864 + Pf6N2E2_5321 0.60 -1.6 6,116,865 - Pf6N2E2_5321 0.60 -0.3 6,116,865 - Pf6N2E2_5321 0.60 -0.2 6,116,865 - Pf6N2E2_5321 0.60 -1.3 6,116,966 + Pf6N2E2_5321 0.71 -0.9 6,116,966 + Pf6N2E2_5321 0.71 -1.9 6,116,967 - Pf6N2E2_5321 0.71 -1.8 6,116,967 - Pf6N2E2_5321 0.71 -1.8 6,117,029 + Pf6N2E2_5321 0.78 -1.8 6,117,029 + Pf6N2E2_5321 0.78 -2.0 6,117,059 + Pf6N2E2_5321 0.82 -1.7 6,117,059 + Pf6N2E2_5321 0.82 -2.1 6,117,059 + Pf6N2E2_5321 0.82 -2.4 6,117,059 + Pf6N2E2_5321 0.82 -1.8 6,117,059 + Pf6N2E2_5321 0.82 -1.7 6,117,059 + Pf6N2E2_5321 0.82 -1.7 6,117,059 + Pf6N2E2_5321 0.82 -2.9 6,117,060 - Pf6N2E2_5321 0.82 -2.2 6,117,060 - Pf6N2E2_5321 0.82 -0.1 6,117,060 - Pf6N2E2_5321 0.82 -0.8 6,117,060 - Pf6N2E2_5321 0.82 -1.3 6,117,060 - Pf6N2E2_5321 0.82 -1.8 6,117,068 + Pf6N2E2_5321 0.83 -0.2 6,117,068 + Pf6N2E2_5321 0.83 -2.6 6,117,068 + Pf6N2E2_5321 0.83 -1.1 6,117,068 + Pf6N2E2_5321 0.83 +0.3 6,117,086 + Pf6N2E2_5321 0.85 -1.5 6,117,209 + +0.6 6,117,209 + +0.2 6,117,209 + -0.2 6,117,209 + +1.8 6,117,210 - -0.2 6,117,210 - -0.2 6,117,218 + +0.3 6,117,218 + -0.8 6,117,218 + +0.9 6,117,219 - -0.5 6,117,219 - +0.1 6,117,219 - +0.6 6,117,219 - +0.2
Or see this region's nucleotide sequence