Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5224 and Pf6N2E2_5225 are separated by 114 nucleotides Pf6N2E2_5225 and Pf6N2E2_5226 are separated by 59 nucleotides Pf6N2E2_5226 and Pf6N2E2_5227 are separated by 27 nucleotides Pf6N2E2_5227 and Pf6N2E2_5228 are separated by 123 nucleotides
Pf6N2E2_5224 - Glutamate racemase (EC 5.1.1.3), at 6,022,588 to 6,023,379
_5224
Pf6N2E2_5225 - Lipid A 3-O-deacylase, at 6,023,494 to 6,024,012
_5225
Pf6N2E2_5226 - FIG00955589: hypothetical protein, at 6,024,072 to 6,024,377
_5226
Pf6N2E2_5227 - Oxidoreductase, short-chain dehydrogenase/reductase family (EC 1.1.1.-), at 6,024,405 to 6,025,178
_5227
Pf6N2E2_5228 - Deoxyribodipyrimidine photolyase (EC 4.1.99.3), at 6,025,302 to 6,026,753
_5228
Position (kb)
6024
6025 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6023.334 kb on + strand at 6023.334 kb on + strand at 6023.334 kb on + strand at 6023.339 kb on + strand at 6023.436 kb on - strand at 6023.437 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.467 kb on - strand at 6023.467 kb on - strand at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.549 kb on - strand, within Pf6N2E2_5225 at 6023.549 kb on - strand, within Pf6N2E2_5225 at 6023.549 kb on - strand, within Pf6N2E2_5225 at 6023.611 kb on + strand, within Pf6N2E2_5225 at 6023.612 kb on - strand, within Pf6N2E2_5225 at 6023.695 kb on + strand, within Pf6N2E2_5225 at 6023.695 kb on + strand, within Pf6N2E2_5225 at 6023.696 kb on - strand, within Pf6N2E2_5225 at 6023.696 kb on - strand, within Pf6N2E2_5225 at 6023.696 kb on - strand, within Pf6N2E2_5225 at 6023.696 kb on - strand, within Pf6N2E2_5225 at 6023.696 kb on - strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.698 kb on - strand, within Pf6N2E2_5225 at 6023.701 kb on + strand, within Pf6N2E2_5225 at 6023.701 kb on + strand, within Pf6N2E2_5225 at 6023.701 kb on + strand, within Pf6N2E2_5225 at 6023.701 kb on + strand, within Pf6N2E2_5225 at 6023.701 kb on + strand, within Pf6N2E2_5225 at 6023.702 kb on - strand, within Pf6N2E2_5225 at 6023.702 kb on - strand, within Pf6N2E2_5225 at 6023.702 kb on - strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.792 kb on - strand, within Pf6N2E2_5225 at 6023.792 kb on - strand, within Pf6N2E2_5225 at 6023.792 kb on - strand, within Pf6N2E2_5225 at 6023.792 kb on - strand, within Pf6N2E2_5225 at 6023.938 kb on + strand, within Pf6N2E2_5225 at 6023.938 kb on + strand, within Pf6N2E2_5225 at 6023.975 kb on + strand at 6023.975 kb on + strand at 6023.975 kb on + strand at 6023.975 kb on + strand at 6023.976 kb on - strand at 6023.976 kb on - strand at 6024.008 kb on + strand at 6024.011 kb on - strand at 6024.011 kb on - strand at 6024.055 kb on + strand at 6024.055 kb on + strand at 6024.055 kb on + strand at 6024.056 kb on - strand at 6024.056 kb on - strand at 6024.056 kb on - strand at 6024.056 kb on - strand at 6024.164 kb on - strand, within Pf6N2E2_5226 at 6024.164 kb on - strand, within Pf6N2E2_5226 at 6024.389 kb on + strand at 6024.390 kb on - strand at 6024.390 kb on - strand at 6024.390 kb on - strand at 6024.390 kb on - strand at 6024.390 kb on - strand at 6024.638 kb on - strand, within Pf6N2E2_5227 at 6024.648 kb on + strand, within Pf6N2E2_5227 at 6024.751 kb on + strand, within Pf6N2E2_5227 at 6024.752 kb on - strand, within Pf6N2E2_5227 at 6024.771 kb on - strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.861 kb on - strand, within Pf6N2E2_5227 at 6024.861 kb on - strand, within Pf6N2E2_5227 at 6024.861 kb on - strand, within Pf6N2E2_5227 at 6024.861 kb on - strand, within Pf6N2E2_5227 at 6024.901 kb on + strand, within Pf6N2E2_5227 at 6024.901 kb on + strand, within Pf6N2E2_5227 at 6024.901 kb on + strand, within Pf6N2E2_5227 at 6024.902 kb on - strand, within Pf6N2E2_5227 at 6025.035 kb on + strand, within Pf6N2E2_5227 at 6025.036 kb on - strand, within Pf6N2E2_5227 at 6025.084 kb on + strand, within Pf6N2E2_5227 at 6025.084 kb on + strand, within Pf6N2E2_5227 at 6025.084 kb on + strand, within Pf6N2E2_5227 at 6025.085 kb on - strand, within Pf6N2E2_5227 at 6025.085 kb on - strand, within Pf6N2E2_5227 at 6025.085 kb on - strand, within Pf6N2E2_5227 at 6025.205 kb on + strand at 6025.205 kb on + strand at 6025.205 kb on + strand at 6025.206 kb on - strand at 6025.206 kb on - strand at 6025.206 kb on - strand at 6025.206 kb on - strand at 6025.206 kb on - strand at 6025.227 kb on - strand at 6025.227 kb on - strand at 6025.244 kb on + strand at 6025.245 kb on - strand at 6025.250 kb on + strand at 6025.250 kb on + strand at 6025.250 kb on + strand at 6025.250 kb on + strand at 6025.250 kb on + strand at 6025.250 kb on + strand at 6025.250 kb on + strand at 6025.251 kb on - strand at 6025.251 kb on - strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.264 kb on + strand at 6025.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 6,023,334 + -1.1 6,023,334 + -1.0 6,023,334 + -1.1 6,023,339 + +0.4 6,023,436 - -0.4 6,023,437 + -0.1 6,023,466 + +0.1 6,023,466 + +0.2 6,023,466 + -0.7 6,023,466 + -0.7 6,023,466 + -0.5 6,023,467 - +0.0 6,023,467 - -0.5 6,023,548 + Pf6N2E2_5225 0.10 -0.9 6,023,548 + Pf6N2E2_5225 0.10 -0.0 6,023,548 + Pf6N2E2_5225 0.10 +0.7 6,023,548 + Pf6N2E2_5225 0.10 +0.6 6,023,548 + Pf6N2E2_5225 0.10 -0.3 6,023,548 + Pf6N2E2_5225 0.10 -0.1 6,023,548 + Pf6N2E2_5225 0.10 +0.1 6,023,548 + Pf6N2E2_5225 0.10 +0.2 6,023,548 + Pf6N2E2_5225 0.10 +0.7 6,023,549 - Pf6N2E2_5225 0.11 -0.0 6,023,549 - Pf6N2E2_5225 0.11 +0.7 6,023,549 - Pf6N2E2_5225 0.11 -0.7 6,023,611 + Pf6N2E2_5225 0.23 -0.0 6,023,612 - Pf6N2E2_5225 0.23 -0.2 6,023,695 + Pf6N2E2_5225 0.39 +0.2 6,023,695 + Pf6N2E2_5225 0.39 +0.6 6,023,696 - Pf6N2E2_5225 0.39 +0.4 6,023,696 - Pf6N2E2_5225 0.39 +0.5 6,023,696 - Pf6N2E2_5225 0.39 +0.7 6,023,696 - Pf6N2E2_5225 0.39 -1.3 6,023,696 - Pf6N2E2_5225 0.39 -0.3 6,023,697 + Pf6N2E2_5225 0.39 +0.9 6,023,697 + Pf6N2E2_5225 0.39 -0.3 6,023,697 + Pf6N2E2_5225 0.39 -1.0 6,023,697 + Pf6N2E2_5225 0.39 +0.4 6,023,697 + Pf6N2E2_5225 0.39 +0.3 6,023,697 + Pf6N2E2_5225 0.39 -0.2 6,023,697 + Pf6N2E2_5225 0.39 +0.9 6,023,697 + Pf6N2E2_5225 0.39 -0.6 6,023,698 - Pf6N2E2_5225 0.39 -0.2 6,023,701 + Pf6N2E2_5225 0.40 -1.7 6,023,701 + Pf6N2E2_5225 0.40 -0.4 6,023,701 + Pf6N2E2_5225 0.40 +0.1 6,023,701 + Pf6N2E2_5225 0.40 -1.2 6,023,701 + Pf6N2E2_5225 0.40 +0.5 6,023,702 - Pf6N2E2_5225 0.40 +0.5 6,023,702 - Pf6N2E2_5225 0.40 +0.1 6,023,702 - Pf6N2E2_5225 0.40 -1.8 6,023,791 + Pf6N2E2_5225 0.57 -0.4 6,023,791 + Pf6N2E2_5225 0.57 +0.1 6,023,791 + Pf6N2E2_5225 0.57 -0.0 6,023,791 + Pf6N2E2_5225 0.57 -0.1 6,023,791 + Pf6N2E2_5225 0.57 +0.9 6,023,791 + Pf6N2E2_5225 0.57 +0.0 6,023,791 + Pf6N2E2_5225 0.57 -0.6 6,023,792 - Pf6N2E2_5225 0.57 -0.7 6,023,792 - Pf6N2E2_5225 0.57 -0.2 6,023,792 - Pf6N2E2_5225 0.57 +0.0 6,023,792 - Pf6N2E2_5225 0.57 -0.0 6,023,938 + Pf6N2E2_5225 0.86 +0.8 6,023,938 + Pf6N2E2_5225 0.86 -0.0 6,023,975 + -0.2 6,023,975 + -0.5 6,023,975 + -0.2 6,023,975 + +0.6 6,023,976 - +0.6 6,023,976 - -1.0 6,024,008 + +0.7 6,024,011 - +0.2 6,024,011 - -0.1 6,024,055 + -1.0 6,024,055 + +0.0 6,024,055 + -0.1 6,024,056 - +1.2 6,024,056 - +0.1 6,024,056 - -0.5 6,024,056 - -0.2 6,024,164 - Pf6N2E2_5226 0.30 -2.0 6,024,164 - Pf6N2E2_5226 0.30 -0.4 6,024,389 + +1.2 6,024,390 - -0.1 6,024,390 - +1.5 6,024,390 - -0.8 6,024,390 - -0.2 6,024,390 - +0.4 6,024,638 - Pf6N2E2_5227 0.30 -0.1 6,024,648 + Pf6N2E2_5227 0.31 +1.1 6,024,751 + Pf6N2E2_5227 0.45 -0.3 6,024,752 - Pf6N2E2_5227 0.45 -0.1 6,024,771 - Pf6N2E2_5227 0.47 -0.9 6,024,860 + Pf6N2E2_5227 0.59 -0.2 6,024,860 + Pf6N2E2_5227 0.59 +0.2 6,024,860 + Pf6N2E2_5227 0.59 +1.1 6,024,860 + Pf6N2E2_5227 0.59 +0.6 6,024,860 + Pf6N2E2_5227 0.59 -0.3 6,024,860 + Pf6N2E2_5227 0.59 -0.1 6,024,861 - Pf6N2E2_5227 0.59 -0.1 6,024,861 - Pf6N2E2_5227 0.59 -0.3 6,024,861 - Pf6N2E2_5227 0.59 +1.1 6,024,861 - Pf6N2E2_5227 0.59 -0.5 6,024,901 + Pf6N2E2_5227 0.64 +0.5 6,024,901 + Pf6N2E2_5227 0.64 -0.2 6,024,901 + Pf6N2E2_5227 0.64 +0.8 6,024,902 - Pf6N2E2_5227 0.64 +0.1 6,025,035 + Pf6N2E2_5227 0.81 -1.0 6,025,036 - Pf6N2E2_5227 0.82 +0.2 6,025,084 + Pf6N2E2_5227 0.88 +0.3 6,025,084 + Pf6N2E2_5227 0.88 +0.7 6,025,084 + Pf6N2E2_5227 0.88 -0.4 6,025,085 - Pf6N2E2_5227 0.88 -0.1 6,025,085 - Pf6N2E2_5227 0.88 +1.0 6,025,085 - Pf6N2E2_5227 0.88 -0.1 6,025,205 + +0.5 6,025,205 + +0.4 6,025,205 + +1.0 6,025,206 - +0.6 6,025,206 - +0.1 6,025,206 - +0.5 6,025,206 - +0.3 6,025,206 - -0.2 6,025,227 - +1.6 6,025,227 - -0.3 6,025,244 + -0.2 6,025,245 - +0.3 6,025,250 + -0.4 6,025,250 + +0.4 6,025,250 + -0.5 6,025,250 + +0.8 6,025,250 + +0.9 6,025,250 + +0.1 6,025,250 + +0.4 6,025,251 - +0.4 6,025,251 - +0.9 6,025,252 + +0.3 6,025,252 + +0.6 6,025,252 + -0.6 6,025,252 + -1.0 6,025,252 + -1.3 6,025,252 + +0.3 6,025,252 + -0.1 6,025,252 + +0.8 6,025,252 + -3.0 6,025,252 + +0.4 6,025,252 + +1.1 6,025,252 + +0.8 6,025,252 + +0.3 6,025,253 - +0.2 6,025,253 - +0.2 6,025,253 - +1.0 6,025,253 - +0.4 6,025,253 - -0.4 6,025,253 - +1.1 6,025,253 - +0.4 6,025,253 - +0.1 6,025,253 - +0.7 6,025,264 + +0.0 6,025,281 - -0.7
Or see this region's nucleotide sequence