Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4982 and Pf6N2E2_4983 are separated by 8 nucleotides Pf6N2E2_4983 and Pf6N2E2_4984 overlap by 8 nucleotides Pf6N2E2_4984 and Pf6N2E2_4985 are separated by 250 nucleotides Pf6N2E2_4985 and Pf6N2E2_4986 are separated by 27 nucleotides
Pf6N2E2_4982 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (EC 6.3.2.-), at 5,749,338 to 5,750,687
_4982
Pf6N2E2_4983 - 3-polyprenyl-4-hydroxybenzoate carboxy-lyase UbiX (EC 4.1.1.-), at 5,750,696 to 5,751,331
_4983
Pf6N2E2_4984 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,751,324 to 5,751,608
_4984
Pf6N2E2_4985 - Nucleoside-diphosphate-sugar epimerases, at 5,751,859 to 5,752,500
_4985
Pf6N2E2_4986 - MORN repeat family protein, at 5,752,528 to 5,754,246
_4986
Position (kb)
5751
5752 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5750.579 kb on + strand at 5751.329 kb on + strand at 5751.329 kb on + strand at 5751.330 kb on - strand at 5751.330 kb on - strand at 5751.330 kb on - strand at 5751.330 kb on - strand at 5751.420 kb on + strand, within Pf6N2E2_4984 at 5751.445 kb on - strand, within Pf6N2E2_4984 at 5751.445 kb on - strand, within Pf6N2E2_4984 at 5751.445 kb on - strand, within Pf6N2E2_4984 at 5751.606 kb on + strand at 5751.634 kb on + strand at 5751.634 kb on + strand at 5751.634 kb on + strand at 5751.634 kb on + strand at 5751.635 kb on - strand at 5751.635 kb on - strand at 5751.635 kb on - strand at 5751.635 kb on - strand at 5751.722 kb on + strand at 5751.723 kb on - strand at 5751.723 kb on - strand at 5751.769 kb on + strand at 5751.786 kb on + strand at 5751.786 kb on + strand at 5751.786 kb on + strand at 5751.786 kb on + strand at 5751.787 kb on - strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.856 kb on + strand at 5751.857 kb on - strand at 5751.857 kb on - strand at 5751.860 kb on + strand at 5751.860 kb on + strand at 5751.860 kb on + strand at 5751.860 kb on + strand at 5751.860 kb on + strand at 5751.860 kb on + strand at 5751.860 kb on + strand at 5751.861 kb on - strand at 5751.861 kb on - strand at 5751.861 kb on - strand at 5751.861 kb on - strand at 5751.861 kb on - strand at 5751.861 kb on - strand at 5751.861 kb on - strand at 5751.873 kb on + strand at 5751.873 kb on + strand at 5751.874 kb on - strand at 5751.972 kb on - strand, within Pf6N2E2_4985 at 5751.972 kb on - strand, within Pf6N2E2_4985 at 5751.972 kb on - strand, within Pf6N2E2_4985 at 5751.972 kb on - strand, within Pf6N2E2_4985 at 5752.127 kb on + strand, within Pf6N2E2_4985 at 5752.128 kb on - strand, within Pf6N2E2_4985 at 5752.128 kb on - strand, within Pf6N2E2_4985 at 5752.128 kb on - strand, within Pf6N2E2_4985 at 5752.496 kb on + strand at 5752.496 kb on + strand at 5752.496 kb on + strand at 5752.496 kb on + strand at 5752.496 kb on + strand at 5752.496 kb on + strand at 5752.496 kb on + strand at 5752.496 kb on + strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.497 kb on - strand at 5752.500 kb on + strand at 5752.500 kb on + strand at 5752.500 kb on + strand at 5752.500 kb on + strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.501 kb on - strand at 5752.518 kb on - strand at 5752.529 kb on + strand at 5752.529 kb on + strand at 5752.530 kb on - strand at 5752.530 kb on - strand at 5752.530 kb on - strand at 5752.578 kb on + strand at 5752.578 kb on + strand at 5752.579 kb on - strand at 5752.579 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 5,750,579 + +2.5 5,751,329 + +0.5 5,751,329 + -0.3 5,751,330 - -0.2 5,751,330 - +1.9 5,751,330 - +0.4 5,751,330 - +0.4 5,751,420 + Pf6N2E2_4984 0.34 -0.5 5,751,445 - Pf6N2E2_4984 0.42 -0.9 5,751,445 - Pf6N2E2_4984 0.42 -0.1 5,751,445 - Pf6N2E2_4984 0.42 -2.8 5,751,606 + -0.2 5,751,634 + +0.7 5,751,634 + -0.8 5,751,634 + +0.5 5,751,634 + +0.1 5,751,635 - -0.2 5,751,635 - +0.1 5,751,635 - +0.1 5,751,635 - -0.4 5,751,722 + +0.5 5,751,723 - -0.1 5,751,723 - +0.4 5,751,769 + +0.8 5,751,786 + +1.1 5,751,786 + +0.3 5,751,786 + -0.5 5,751,786 + -0.2 5,751,787 - -0.5 5,751,856 + -1.1 5,751,856 + +0.9 5,751,856 + -1.1 5,751,856 + -0.3 5,751,856 + +0.3 5,751,856 + +1.7 5,751,856 + -1.5 5,751,856 + +0.3 5,751,856 + -0.2 5,751,857 - +0.6 5,751,857 - -0.3 5,751,860 + -0.6 5,751,860 + -0.8 5,751,860 + -0.3 5,751,860 + +1.3 5,751,860 + -0.9 5,751,860 + -1.3 5,751,860 + -0.6 5,751,861 - -0.1 5,751,861 - -0.8 5,751,861 - +0.4 5,751,861 - -0.0 5,751,861 - -0.0 5,751,861 - -0.2 5,751,861 - +0.2 5,751,873 + -0.3 5,751,873 + +0.0 5,751,874 - -0.1 5,751,972 - Pf6N2E2_4985 0.18 +0.9 5,751,972 - Pf6N2E2_4985 0.18 -0.1 5,751,972 - Pf6N2E2_4985 0.18 -0.4 5,751,972 - Pf6N2E2_4985 0.18 +0.8 5,752,127 + Pf6N2E2_4985 0.42 +0.6 5,752,128 - Pf6N2E2_4985 0.42 -1.1 5,752,128 - Pf6N2E2_4985 0.42 +0.2 5,752,128 - Pf6N2E2_4985 0.42 +0.6 5,752,496 + +0.0 5,752,496 + +0.9 5,752,496 + +1.0 5,752,496 + -0.1 5,752,496 + +0.0 5,752,496 + +0.2 5,752,496 + +0.6 5,752,496 + -1.3 5,752,497 - -0.5 5,752,497 - -0.2 5,752,497 - -0.5 5,752,497 - +0.5 5,752,497 - +0.8 5,752,497 - +0.6 5,752,497 - -0.4 5,752,497 - -0.2 5,752,497 - +0.2 5,752,497 - +0.5 5,752,497 - -0.3 5,752,497 - -1.5 5,752,500 + +1.4 5,752,500 + -0.5 5,752,500 + +0.5 5,752,500 + +0.6 5,752,501 - -1.0 5,752,501 - +0.0 5,752,501 - -0.0 5,752,501 - -0.6 5,752,501 - +0.8 5,752,501 - +0.9 5,752,501 - -0.5 5,752,501 - +0.3 5,752,501 - +0.0 5,752,501 - +0.4 5,752,518 - -0.2 5,752,529 + +0.3 5,752,529 + +0.2 5,752,530 - +0.1 5,752,530 - +0.9 5,752,530 - +0.9 5,752,578 + +0.1 5,752,578 + +0.7 5,752,579 - +0.3 5,752,579 - +0.1
Or see this region's nucleotide sequence