Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4610 and Pf6N2E2_4611 are separated by 46 nucleotides Pf6N2E2_4611 and Pf6N2E2_4612 are separated by 77 nucleotides
Pf6N2E2_4610 - Mobile element protein, at 5,334,708 to 5,335,877
_4610
Pf6N2E2_4611 - Ferric siderophore transport system, periplasmic binding protein TonB, at 5,335,924 to 5,336,820
_4611
Pf6N2E2_4612 - Glutathione synthetase (EC 6.3.2.3), at 5,336,898 to 5,337,851
_4612
Position (kb) 5335
5336
5337 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5335.862 kb on + strand at 5335.896 kb on + strand at 5335.896 kb on + strand at 5335.896 kb on + strand at 5335.911 kb on + strand at 5335.911 kb on + strand at 5335.912 kb on - strand at 5335.912 kb on - strand at 5335.912 kb on - strand at 5336.083 kb on + strand, within Pf6N2E2_4611 at 5336.083 kb on + strand, within Pf6N2E2_4611 at 5336.099 kb on + strand, within Pf6N2E2_4611 at 5336.099 kb on + strand, within Pf6N2E2_4611 at 5336.100 kb on - strand, within Pf6N2E2_4611 at 5336.148 kb on - strand, within Pf6N2E2_4611 at 5336.291 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.863 kb on + strand at 5337.188 kb on - strand, within Pf6N2E2_4612 at 5337.188 kb on - strand, within Pf6N2E2_4612 at 5337.250 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.551 kb on - strand, within Pf6N2E2_4612 at 5337.551 kb on - strand, within Pf6N2E2_4612 at 5337.551 kb on - strand, within Pf6N2E2_4612 at 5337.559 kb on + strand, within Pf6N2E2_4612 at 5337.559 kb on + strand, within Pf6N2E2_4612 at 5337.740 kb on - strand, within Pf6N2E2_4612 at 5337.740 kb on - strand, within Pf6N2E2_4612 at 5337.792 kb on - strand at 5337.800 kb on + strand at 5337.803 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 5,335,862 + +1.1 5,335,896 + -0.0 5,335,896 + +0.7 5,335,896 + +0.7 5,335,911 + +0.1 5,335,911 + -0.2 5,335,912 - +0.4 5,335,912 - +0.0 5,335,912 - -0.3 5,336,083 + Pf6N2E2_4611 0.18 -0.8 5,336,083 + Pf6N2E2_4611 0.18 -1.2 5,336,099 + Pf6N2E2_4611 0.20 -1.0 5,336,099 + Pf6N2E2_4611 0.20 -1.3 5,336,100 - Pf6N2E2_4611 0.20 -1.5 5,336,148 - Pf6N2E2_4611 0.25 +0.0 5,336,291 + Pf6N2E2_4611 0.41 -0.9 5,336,517 + Pf6N2E2_4611 0.66 -0.3 5,336,517 + Pf6N2E2_4611 0.66 -0.9 5,336,517 + Pf6N2E2_4611 0.66 -1.9 5,336,517 + Pf6N2E2_4611 0.66 -2.0 5,336,517 + Pf6N2E2_4611 0.66 -1.6 5,336,517 + Pf6N2E2_4611 0.66 -1.0 5,336,863 + -0.1 5,337,188 - Pf6N2E2_4612 0.30 -0.6 5,337,188 - Pf6N2E2_4612 0.30 -1.6 5,337,250 + Pf6N2E2_4612 0.37 -0.6 5,337,550 + Pf6N2E2_4612 0.68 -0.9 5,337,550 + Pf6N2E2_4612 0.68 -0.9 5,337,550 + Pf6N2E2_4612 0.68 -2.0 5,337,550 + Pf6N2E2_4612 0.68 -0.1 5,337,550 + Pf6N2E2_4612 0.68 -1.0 5,337,551 - Pf6N2E2_4612 0.68 -3.7 5,337,551 - Pf6N2E2_4612 0.68 -2.3 5,337,551 - Pf6N2E2_4612 0.68 -3.5 5,337,559 + Pf6N2E2_4612 0.69 -1.9 5,337,559 + Pf6N2E2_4612 0.69 -1.9 5,337,740 - Pf6N2E2_4612 0.88 -2.2 5,337,740 - Pf6N2E2_4612 0.88 -1.3 5,337,792 - -0.8 5,337,800 + -2.2 5,337,803 - -1.7
Or see this region's nucleotide sequence