Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4485 and Pf6N2E2_4486 are separated by 53 nucleotides Pf6N2E2_4486 and Pf6N2E2_4487 are separated by 355 nucleotides
Pf6N2E2_4485 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 5,218,266 to 5,219,348
_4485
Pf6N2E2_4486 - Glycine cleavage system H protein, at 5,219,402 to 5,219,785
_4486
Pf6N2E2_4487 - YD repeat protein, at 5,220,141 to 5,221,238
_4487
Position (kb)
5219
5220 Strain fitness (log2 ratio)
-2
-1
0
1 at 5218.412 kb on - strand, within Pf6N2E2_4485 at 5218.412 kb on - strand, within Pf6N2E2_4485 at 5218.412 kb on - strand, within Pf6N2E2_4485 at 5218.518 kb on + strand, within Pf6N2E2_4485 at 5218.518 kb on + strand, within Pf6N2E2_4485 at 5218.519 kb on - strand, within Pf6N2E2_4485 at 5218.565 kb on + strand, within Pf6N2E2_4485 at 5218.565 kb on + strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.566 kb on - strand, within Pf6N2E2_4485 at 5218.569 kb on + strand, within Pf6N2E2_4485 at 5218.569 kb on + strand, within Pf6N2E2_4485 at 5218.569 kb on + strand, within Pf6N2E2_4485 at 5218.570 kb on - strand, within Pf6N2E2_4485 at 5218.570 kb on - strand, within Pf6N2E2_4485 at 5218.570 kb on - strand, within Pf6N2E2_4485 at 5218.827 kb on + strand, within Pf6N2E2_4485 at 5218.969 kb on - strand, within Pf6N2E2_4485 at 5219.095 kb on + strand, within Pf6N2E2_4485 at 5219.096 kb on - strand, within Pf6N2E2_4485 at 5219.096 kb on - strand, within Pf6N2E2_4485 at 5219.096 kb on - strand, within Pf6N2E2_4485 at 5219.096 kb on - strand, within Pf6N2E2_4485 at 5219.096 kb on - strand, within Pf6N2E2_4485 at 5219.198 kb on + strand, within Pf6N2E2_4485 at 5219.198 kb on + strand, within Pf6N2E2_4485 at 5219.198 kb on + strand, within Pf6N2E2_4485 at 5219.199 kb on - strand, within Pf6N2E2_4485 at 5219.210 kb on + strand, within Pf6N2E2_4485 at 5219.211 kb on - strand, within Pf6N2E2_4485 at 5219.211 kb on - strand, within Pf6N2E2_4485 at 5219.216 kb on + strand, within Pf6N2E2_4485 at 5219.217 kb on - strand, within Pf6N2E2_4485 at 5219.273 kb on - strand at 5219.285 kb on + strand at 5219.286 kb on - strand at 5219.346 kb on + strand at 5219.346 kb on + strand at 5219.347 kb on - strand at 5219.347 kb on - strand at 5219.347 kb on - strand at 5219.385 kb on + strand at 5219.385 kb on + strand at 5219.386 kb on - strand at 5219.386 kb on - strand at 5219.401 kb on + strand at 5219.401 kb on + strand at 5219.401 kb on + strand at 5219.401 kb on + strand at 5219.401 kb on + strand at 5219.401 kb on + strand at 5219.402 kb on - strand at 5219.402 kb on - strand at 5219.410 kb on + strand at 5219.612 kb on + strand, within Pf6N2E2_4486 at 5219.612 kb on + strand, within Pf6N2E2_4486 at 5219.612 kb on + strand, within Pf6N2E2_4486 at 5219.612 kb on + strand, within Pf6N2E2_4486 at 5219.612 kb on + strand, within Pf6N2E2_4486 at 5219.613 kb on - strand, within Pf6N2E2_4486 at 5219.613 kb on - strand, within Pf6N2E2_4486 at 5219.613 kb on - strand, within Pf6N2E2_4486 at 5219.613 kb on - strand, within Pf6N2E2_4486 at 5219.613 kb on - strand, within Pf6N2E2_4486 at 5219.690 kb on + strand, within Pf6N2E2_4486 at 5219.740 kb on + strand, within Pf6N2E2_4486 at 5219.762 kb on + strand at 5219.773 kb on + strand at 5219.905 kb on + strand at 5219.906 kb on - strand at 5219.921 kb on - strand at 5219.921 kb on - strand at 5220.032 kb on - strand at 5220.033 kb on + strand at 5220.033 kb on + strand at 5220.033 kb on + strand at 5220.034 kb on - strand at 5220.034 kb on - strand at 5220.034 kb on - strand at 5220.034 kb on - strand at 5220.034 kb on - strand at 5220.034 kb on - strand at 5220.034 kb on - strand at 5220.036 kb on + strand at 5220.036 kb on + strand at 5220.049 kb on - strand at 5220.068 kb on + strand at 5220.068 kb on + strand at 5220.108 kb on - strand at 5220.111 kb on + strand at 5220.112 kb on - strand at 5220.112 kb on - strand at 5220.112 kb on - strand at 5220.124 kb on + strand at 5220.124 kb on + strand at 5220.213 kb on + strand at 5220.235 kb on + strand at 5220.302 kb on + strand, within Pf6N2E2_4487 at 5220.302 kb on + strand, within Pf6N2E2_4487 at 5220.346 kb on + strand, within Pf6N2E2_4487 at 5220.346 kb on + strand, within Pf6N2E2_4487 at 5220.438 kb on + strand, within Pf6N2E2_4487 at 5220.438 kb on + strand, within Pf6N2E2_4487 at 5220.438 kb on + strand, within Pf6N2E2_4487 at 5220.438 kb on + strand, within Pf6N2E2_4487 at 5220.438 kb on + strand, within Pf6N2E2_4487 at 5220.438 kb on + strand, within Pf6N2E2_4487 at 5220.439 kb on - strand, within Pf6N2E2_4487 at 5220.439 kb on - strand, within Pf6N2E2_4487 at 5220.439 kb on - strand, within Pf6N2E2_4487 at 5220.439 kb on - strand, within Pf6N2E2_4487 at 5220.440 kb on + strand, within Pf6N2E2_4487 at 5220.440 kb on + strand, within Pf6N2E2_4487 at 5220.441 kb on - strand, within Pf6N2E2_4487 at 5220.441 kb on - strand, within Pf6N2E2_4487 at 5220.528 kb on + strand, within Pf6N2E2_4487 at 5220.528 kb on + strand, within Pf6N2E2_4487 at 5220.528 kb on + strand, within Pf6N2E2_4487 at 5220.528 kb on + strand, within Pf6N2E2_4487 at 5220.529 kb on - strand, within Pf6N2E2_4487 at 5220.529 kb on - strand, within Pf6N2E2_4487 at 5220.529 kb on - strand, within Pf6N2E2_4487 at 5220.532 kb on + strand, within Pf6N2E2_4487 at 5220.532 kb on + strand, within Pf6N2E2_4487 at 5220.533 kb on - strand, within Pf6N2E2_4487 at 5220.555 kb on + strand, within Pf6N2E2_4487 at 5220.555 kb on + strand, within Pf6N2E2_4487 at 5220.556 kb on - strand, within Pf6N2E2_4487 at 5220.556 kb on - strand, within Pf6N2E2_4487 at 5220.556 kb on - strand, within Pf6N2E2_4487 at 5220.557 kb on + strand, within Pf6N2E2_4487 at 5220.557 kb on + strand, within Pf6N2E2_4487 at 5220.557 kb on + strand, within Pf6N2E2_4487 at 5220.558 kb on - strand, within Pf6N2E2_4487 at 5220.633 kb on + strand, within Pf6N2E2_4487 at 5220.633 kb on + strand, within Pf6N2E2_4487 at 5220.633 kb on + strand, within Pf6N2E2_4487 at 5220.633 kb on + strand, within Pf6N2E2_4487 at 5220.634 kb on - strand, within Pf6N2E2_4487 at 5220.634 kb on - strand, within Pf6N2E2_4487 at 5220.634 kb on - strand, within Pf6N2E2_4487 at 5220.653 kb on + strand, within Pf6N2E2_4487 at 5220.654 kb on - strand, within Pf6N2E2_4487 at 5220.656 kb on + strand, within Pf6N2E2_4487 at 5220.656 kb on + strand, within Pf6N2E2_4487 at 5220.657 kb on - strand, within Pf6N2E2_4487 at 5220.668 kb on + strand, within Pf6N2E2_4487 at 5220.686 kb on - strand, within Pf6N2E2_4487 at 5220.698 kb on + strand, within Pf6N2E2_4487 at 5220.707 kb on + strand, within Pf6N2E2_4487 at 5220.708 kb on - strand, within Pf6N2E2_4487 at 5220.708 kb on - strand, within Pf6N2E2_4487 at 5220.711 kb on + strand, within Pf6N2E2_4487 at 5220.719 kb on + strand, within Pf6N2E2_4487 at 5220.738 kb on + strand, within Pf6N2E2_4487 at 5220.741 kb on + strand, within Pf6N2E2_4487 at 5220.743 kb on + strand, within Pf6N2E2_4487
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 5,218,412 - Pf6N2E2_4485 0.13 +0.6 5,218,412 - Pf6N2E2_4485 0.13 -0.2 5,218,412 - Pf6N2E2_4485 0.13 +1.1 5,218,518 + Pf6N2E2_4485 0.23 +0.4 5,218,518 + Pf6N2E2_4485 0.23 +0.6 5,218,519 - Pf6N2E2_4485 0.23 +0.4 5,218,565 + Pf6N2E2_4485 0.28 +1.0 5,218,565 + Pf6N2E2_4485 0.28 +0.7 5,218,566 - Pf6N2E2_4485 0.28 -0.1 5,218,566 - Pf6N2E2_4485 0.28 +1.2 5,218,566 - Pf6N2E2_4485 0.28 +1.6 5,218,566 - Pf6N2E2_4485 0.28 -0.4 5,218,566 - Pf6N2E2_4485 0.28 +0.2 5,218,566 - Pf6N2E2_4485 0.28 -0.1 5,218,566 - Pf6N2E2_4485 0.28 +1.1 5,218,566 - Pf6N2E2_4485 0.28 +0.4 5,218,569 + Pf6N2E2_4485 0.28 -0.2 5,218,569 + Pf6N2E2_4485 0.28 +0.3 5,218,569 + Pf6N2E2_4485 0.28 +0.2 5,218,570 - Pf6N2E2_4485 0.28 +0.3 5,218,570 - Pf6N2E2_4485 0.28 +0.7 5,218,570 - Pf6N2E2_4485 0.28 +1.1 5,218,827 + Pf6N2E2_4485 0.52 +0.5 5,218,969 - Pf6N2E2_4485 0.65 +0.6 5,219,095 + Pf6N2E2_4485 0.77 +0.0 5,219,096 - Pf6N2E2_4485 0.77 +1.5 5,219,096 - Pf6N2E2_4485 0.77 +0.9 5,219,096 - Pf6N2E2_4485 0.77 +1.0 5,219,096 - Pf6N2E2_4485 0.77 +0.8 5,219,096 - Pf6N2E2_4485 0.77 +0.1 5,219,198 + Pf6N2E2_4485 0.86 +0.6 5,219,198 + Pf6N2E2_4485 0.86 +0.6 5,219,198 + Pf6N2E2_4485 0.86 +0.9 5,219,199 - Pf6N2E2_4485 0.86 +1.2 5,219,210 + Pf6N2E2_4485 0.87 +0.8 5,219,211 - Pf6N2E2_4485 0.87 +0.2 5,219,211 - Pf6N2E2_4485 0.87 +0.8 5,219,216 + Pf6N2E2_4485 0.88 +0.2 5,219,217 - Pf6N2E2_4485 0.88 +0.8 5,219,273 - +1.5 5,219,285 + +0.9 5,219,286 - +0.0 5,219,346 + +0.7 5,219,346 + +0.4 5,219,347 - +0.7 5,219,347 - -1.0 5,219,347 - +0.1 5,219,385 + +0.3 5,219,385 + +1.0 5,219,386 - +0.8 5,219,386 - +0.4 5,219,401 + -1.9 5,219,401 + -0.5 5,219,401 + +1.1 5,219,401 + -0.1 5,219,401 + +0.0 5,219,401 + -0.8 5,219,402 - -0.3 5,219,402 - -1.0 5,219,410 + -1.8 5,219,612 + Pf6N2E2_4486 0.55 +0.4 5,219,612 + Pf6N2E2_4486 0.55 +1.1 5,219,612 + Pf6N2E2_4486 0.55 -1.6 5,219,612 + Pf6N2E2_4486 0.55 -2.2 5,219,612 + Pf6N2E2_4486 0.55 -2.1 5,219,613 - Pf6N2E2_4486 0.55 -0.5 5,219,613 - Pf6N2E2_4486 0.55 -1.1 5,219,613 - Pf6N2E2_4486 0.55 -0.2 5,219,613 - Pf6N2E2_4486 0.55 -1.0 5,219,613 - Pf6N2E2_4486 0.55 +0.7 5,219,690 + Pf6N2E2_4486 0.75 -1.4 5,219,740 + Pf6N2E2_4486 0.88 -1.6 5,219,762 + -0.9 5,219,773 + +0.0 5,219,905 + +0.5 5,219,906 - +0.3 5,219,921 - -0.8 5,219,921 - +0.5 5,220,032 - +0.0 5,220,033 + +0.2 5,220,033 + +0.0 5,220,033 + -0.4 5,220,034 - -0.4 5,220,034 - +1.1 5,220,034 - -0.0 5,220,034 - +0.6 5,220,034 - -0.5 5,220,034 - +0.8 5,220,034 - +1.7 5,220,036 + +0.8 5,220,036 + +0.3 5,220,049 - +0.3 5,220,068 + -0.5 5,220,068 + +0.1 5,220,108 - +0.0 5,220,111 + -0.2 5,220,112 - +0.8 5,220,112 - +0.1 5,220,112 - +1.2 5,220,124 + -0.7 5,220,124 + +1.5 5,220,213 + -0.4 5,220,235 + +0.2 5,220,302 + Pf6N2E2_4487 0.15 +0.5 5,220,302 + Pf6N2E2_4487 0.15 +0.5 5,220,346 + Pf6N2E2_4487 0.19 +1.2 5,220,346 + Pf6N2E2_4487 0.19 +0.2 5,220,438 + Pf6N2E2_4487 0.27 -0.1 5,220,438 + Pf6N2E2_4487 0.27 -0.3 5,220,438 + Pf6N2E2_4487 0.27 +0.1 5,220,438 + Pf6N2E2_4487 0.27 +0.3 5,220,438 + Pf6N2E2_4487 0.27 +0.6 5,220,438 + Pf6N2E2_4487 0.27 +0.9 5,220,439 - Pf6N2E2_4487 0.27 +0.9 5,220,439 - Pf6N2E2_4487 0.27 +0.2 5,220,439 - Pf6N2E2_4487 0.27 -0.3 5,220,439 - Pf6N2E2_4487 0.27 +0.5 5,220,440 + Pf6N2E2_4487 0.27 +0.0 5,220,440 + Pf6N2E2_4487 0.27 -0.5 5,220,441 - Pf6N2E2_4487 0.27 +1.3 5,220,441 - Pf6N2E2_4487 0.27 +0.1 5,220,528 + Pf6N2E2_4487 0.35 +0.1 5,220,528 + Pf6N2E2_4487 0.35 +0.5 5,220,528 + Pf6N2E2_4487 0.35 -0.3 5,220,528 + Pf6N2E2_4487 0.35 -0.2 5,220,529 - Pf6N2E2_4487 0.35 +0.5 5,220,529 - Pf6N2E2_4487 0.35 -0.0 5,220,529 - Pf6N2E2_4487 0.35 +0.5 5,220,532 + Pf6N2E2_4487 0.36 -1.4 5,220,532 + Pf6N2E2_4487 0.36 -0.1 5,220,533 - Pf6N2E2_4487 0.36 -1.3 5,220,555 + Pf6N2E2_4487 0.38 +0.2 5,220,555 + Pf6N2E2_4487 0.38 +0.4 5,220,556 - Pf6N2E2_4487 0.38 +0.7 5,220,556 - Pf6N2E2_4487 0.38 +0.5 5,220,556 - Pf6N2E2_4487 0.38 +0.5 5,220,557 + Pf6N2E2_4487 0.38 -0.9 5,220,557 + Pf6N2E2_4487 0.38 +0.2 5,220,557 + Pf6N2E2_4487 0.38 -0.2 5,220,558 - Pf6N2E2_4487 0.38 -0.3 5,220,633 + Pf6N2E2_4487 0.45 -0.0 5,220,633 + Pf6N2E2_4487 0.45 +0.6 5,220,633 + Pf6N2E2_4487 0.45 +0.5 5,220,633 + Pf6N2E2_4487 0.45 -0.1 5,220,634 - Pf6N2E2_4487 0.45 -0.6 5,220,634 - Pf6N2E2_4487 0.45 -0.1 5,220,634 - Pf6N2E2_4487 0.45 +0.3 5,220,653 + Pf6N2E2_4487 0.47 +0.2 5,220,654 - Pf6N2E2_4487 0.47 -0.4 5,220,656 + Pf6N2E2_4487 0.47 -0.8 5,220,656 + Pf6N2E2_4487 0.47 +0.6 5,220,657 - Pf6N2E2_4487 0.47 +1.2 5,220,668 + Pf6N2E2_4487 0.48 +0.2 5,220,686 - Pf6N2E2_4487 0.50 -1.3 5,220,698 + Pf6N2E2_4487 0.51 +0.1 5,220,707 + Pf6N2E2_4487 0.52 -0.0 5,220,708 - Pf6N2E2_4487 0.52 +0.5 5,220,708 - Pf6N2E2_4487 0.52 +0.1 5,220,711 + Pf6N2E2_4487 0.52 +0.1 5,220,719 + Pf6N2E2_4487 0.53 +0.6 5,220,738 + Pf6N2E2_4487 0.54 +0.3 5,220,741 + Pf6N2E2_4487 0.55 -0.3 5,220,743 + Pf6N2E2_4487 0.55 +0.8
Or see this region's nucleotide sequence