Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4161 and Pf6N2E2_4162 are separated by 374 nucleotides Pf6N2E2_4162 and Pf6N2E2_4163 are separated by 74 nucleotides Pf6N2E2_4163 and Pf6N2E2_4164 are separated by 59 nucleotides
Pf6N2E2_4161 - GTP-binding protein EngB, at 4,842,302 to 4,842,943
_4161
Pf6N2E2_4162 - DNA polymerase I (EC 2.7.7.7), at 4,843,318 to 4,846,086
_4162
Pf6N2E2_4163 - FIG00953549: hypothetical protein, at 4,846,161 to 4,846,448
_4163
Pf6N2E2_4164 - Homoserine kinase (EC 2.7.1.39), at 4,846,508 to 4,847,458
_4164
Position (kb)
4843
4844
4845
4846
4847 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4842.942 kb on - strand at 4842.979 kb on - strand at 4843.034 kb on + strand at 4843.034 kb on + strand at 4843.034 kb on + strand at 4843.034 kb on + strand at 4843.048 kb on + strand at 4843.048 kb on + strand at 4843.048 kb on + strand at 4843.048 kb on + strand at 4843.048 kb on + strand at 4843.048 kb on + strand at 4843.048 kb on + strand at 4843.049 kb on - strand at 4843.049 kb on - strand at 4843.049 kb on - strand at 4843.049 kb on - strand at 4843.049 kb on - strand at 4843.049 kb on - strand at 4843.049 kb on - strand at 4843.049 kb on - strand at 4843.058 kb on + strand at 4843.058 kb on + strand at 4843.058 kb on + strand at 4843.058 kb on + strand at 4843.058 kb on + strand at 4843.058 kb on + strand at 4843.058 kb on + strand at 4843.058 kb on + strand at 4843.059 kb on - strand at 4843.059 kb on - strand at 4843.059 kb on - strand at 4843.059 kb on - strand at 4843.059 kb on - strand at 4843.059 kb on - strand at 4843.059 kb on - strand at 4843.117 kb on + strand at 4843.118 kb on - strand at 4843.127 kb on + strand at 4843.139 kb on + strand at 4843.140 kb on - strand at 4843.140 kb on - strand at 4843.161 kb on + strand at 4843.161 kb on + strand at 4843.162 kb on - strand at 4843.162 kb on - strand at 4843.162 kb on - strand at 4843.162 kb on - strand at 4843.192 kb on + strand at 4843.192 kb on + strand at 4843.192 kb on + strand at 4843.192 kb on + strand at 4843.192 kb on + strand at 4843.192 kb on + strand at 4843.192 kb on + strand at 4843.192 kb on + strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.193 kb on - strand at 4843.313 kb on - strand at 4844.187 kb on - strand, within Pf6N2E2_4162 at 4844.187 kb on - strand, within Pf6N2E2_4162 at 4844.768 kb on + strand, within Pf6N2E2_4162 at 4844.768 kb on + strand, within Pf6N2E2_4162 at 4845.144 kb on - strand, within Pf6N2E2_4162 at 4845.144 kb on - strand, within Pf6N2E2_4162 at 4846.121 kb on - strand at 4846.157 kb on + strand at 4846.157 kb on + strand at 4846.158 kb on - strand at 4846.166 kb on + strand at 4846.166 kb on + strand at 4846.166 kb on + strand at 4846.166 kb on + strand at 4846.167 kb on - strand at 4846.167 kb on - strand at 4846.171 kb on + strand at 4846.171 kb on + strand at 4846.171 kb on + strand at 4846.172 kb on - strand at 4846.280 kb on + strand, within Pf6N2E2_4163 at 4846.318 kb on - strand, within Pf6N2E2_4163 at 4846.353 kb on + strand, within Pf6N2E2_4163 at 4846.353 kb on + strand, within Pf6N2E2_4163 at 4846.353 kb on + strand, within Pf6N2E2_4163 at 4846.353 kb on + strand, within Pf6N2E2_4163 at 4846.353 kb on + strand, within Pf6N2E2_4163 at 4846.435 kb on + strand at 4846.435 kb on + strand at 4846.446 kb on + strand at 4846.446 kb on + strand at 4846.446 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 4,842,942 - +0.2 4,842,979 - +0.4 4,843,034 + +1.2 4,843,034 + +0.0 4,843,034 + -0.3 4,843,034 + +2.2 4,843,048 + +0.4 4,843,048 + -1.1 4,843,048 + -1.7 4,843,048 + -0.7 4,843,048 + -0.5 4,843,048 + -0.2 4,843,048 + -0.2 4,843,049 - +0.1 4,843,049 - +0.9 4,843,049 - -0.3 4,843,049 - +0.1 4,843,049 - -1.0 4,843,049 - -1.7 4,843,049 - +0.0 4,843,049 - -0.2 4,843,058 + -0.8 4,843,058 + +0.5 4,843,058 + -0.4 4,843,058 + +1.1 4,843,058 + -0.1 4,843,058 + -0.1 4,843,058 + +0.3 4,843,058 + +0.3 4,843,059 - -0.4 4,843,059 - +0.6 4,843,059 - +0.5 4,843,059 - +0.7 4,843,059 - +0.0 4,843,059 - +0.0 4,843,059 - -0.1 4,843,117 + -0.7 4,843,118 - +1.7 4,843,127 + -1.5 4,843,139 + -0.7 4,843,140 - +0.2 4,843,140 - +0.7 4,843,161 + -0.5 4,843,161 + +0.1 4,843,162 - +0.7 4,843,162 - +1.3 4,843,162 - +0.4 4,843,162 - +0.4 4,843,192 + +0.4 4,843,192 + -0.6 4,843,192 + -0.6 4,843,192 + -0.1 4,843,192 + +0.4 4,843,192 + -0.6 4,843,192 + -1.2 4,843,192 + +0.1 4,843,193 - -1.0 4,843,193 - -0.4 4,843,193 - +0.9 4,843,193 - +0.0 4,843,193 - -1.0 4,843,193 - +1.0 4,843,193 - +1.0 4,843,193 - +0.7 4,843,193 - +0.1 4,843,193 - +0.3 4,843,193 - +0.5 4,843,193 - -0.9 4,843,193 - +0.2 4,843,193 - -0.7 4,843,193 - -0.7 4,843,313 - +0.1 4,844,187 - Pf6N2E2_4162 0.31 +0.8 4,844,187 - Pf6N2E2_4162 0.31 +0.0 4,844,768 + Pf6N2E2_4162 0.52 -0.7 4,844,768 + Pf6N2E2_4162 0.52 -0.4 4,845,144 - Pf6N2E2_4162 0.66 -1.1 4,845,144 - Pf6N2E2_4162 0.66 -0.8 4,846,121 - -1.3 4,846,157 + -0.3 4,846,157 + -0.7 4,846,158 - +0.2 4,846,166 + +0.5 4,846,166 + +0.3 4,846,166 + -0.1 4,846,166 + -0.9 4,846,167 - +1.2 4,846,167 - +1.0 4,846,171 + +0.2 4,846,171 + -1.9 4,846,171 + -1.0 4,846,172 - -0.1 4,846,280 + Pf6N2E2_4163 0.41 +0.3 4,846,318 - Pf6N2E2_4163 0.55 -0.6 4,846,353 + Pf6N2E2_4163 0.67 -0.8 4,846,353 + Pf6N2E2_4163 0.67 +0.0 4,846,353 + Pf6N2E2_4163 0.67 +1.1 4,846,353 + Pf6N2E2_4163 0.67 +0.0 4,846,353 + Pf6N2E2_4163 0.67 +0.2 4,846,435 + +1.3 4,846,435 + +0.1 4,846,446 + -1.0 4,846,446 + -0.4 4,846,446 + +0.1
Or see this region's nucleotide sequence