Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3843 and Pf6N2E2_3844 are separated by 261 nucleotides Pf6N2E2_3844 and Pf6N2E2_3845 are separated by 270 nucleotides Pf6N2E2_3845 and Pf6N2E2_3846 overlap by 4 nucleotides
Pf6N2E2_3843 - MFS permease, at 4,474,717 to 4,476,378
_3843
Pf6N2E2_3844 - hypothetical protein, at 4,476,640 to 4,477,038
_3844
Pf6N2E2_3845 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 4,477,309 to 4,479,540
_3845
Pf6N2E2_3846 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 4,479,537 to 4,480,604
_3846
Position (kb)
4477
4478
4479
4480 Strain fitness (log2 ratio)
-2
-1
0
1 at 4476.383 kb on + strand at 4476.395 kb on - strand at 4476.455 kb on + strand at 4476.456 kb on - strand at 4476.456 kb on - strand at 4476.476 kb on - strand at 4476.522 kb on + strand at 4476.555 kb on + strand at 4476.742 kb on - strand, within Pf6N2E2_3844 at 4476.875 kb on + strand, within Pf6N2E2_3844 at 4476.875 kb on + strand, within Pf6N2E2_3844 at 4477.036 kb on + strand at 4477.036 kb on + strand at 4477.037 kb on - strand at 4477.037 kb on - strand at 4477.037 kb on - strand at 4477.037 kb on - strand at 4477.037 kb on - strand at 4477.040 kb on + strand at 4477.041 kb on - strand at 4477.041 kb on - strand at 4477.251 kb on + strand at 4477.251 kb on + strand at 4477.252 kb on - strand at 4477.264 kb on + strand at 4477.266 kb on + strand at 4477.267 kb on - strand at 4477.267 kb on - strand at 4477.267 kb on - strand at 4477.294 kb on + strand at 4477.294 kb on + strand at 4477.294 kb on + strand at 4477.294 kb on + strand at 4477.294 kb on + strand at 4477.295 kb on - strand at 4477.295 kb on - strand at 4477.295 kb on - strand at 4477.340 kb on + strand at 4477.340 kb on + strand at 4477.341 kb on - strand at 4477.341 kb on - strand at 4477.411 kb on + strand at 4477.411 kb on + strand at 4477.412 kb on - strand at 4477.412 kb on - strand at 4477.412 kb on - strand at 4477.412 kb on - strand at 4477.599 kb on - strand, within Pf6N2E2_3845 at 4477.619 kb on + strand, within Pf6N2E2_3845 at 4477.856 kb on - strand, within Pf6N2E2_3845 at 4477.856 kb on - strand, within Pf6N2E2_3845 at 4477.856 kb on - strand, within Pf6N2E2_3845 at 4477.988 kb on + strand, within Pf6N2E2_3845 at 4477.989 kb on - strand, within Pf6N2E2_3845 at 4478.038 kb on + strand, within Pf6N2E2_3845 at 4478.038 kb on + strand, within Pf6N2E2_3845 at 4478.039 kb on - strand, within Pf6N2E2_3845 at 4478.601 kb on + strand, within Pf6N2E2_3845 at 4478.601 kb on + strand, within Pf6N2E2_3845 at 4478.602 kb on - strand, within Pf6N2E2_3845 at 4478.602 kb on - strand, within Pf6N2E2_3845 at 4478.803 kb on + strand, within Pf6N2E2_3845 at 4478.803 kb on + strand, within Pf6N2E2_3845 at 4478.804 kb on - strand, within Pf6N2E2_3845 at 4478.804 kb on - strand, within Pf6N2E2_3845 at 4478.804 kb on - strand, within Pf6N2E2_3845 at 4478.851 kb on - strand, within Pf6N2E2_3845 at 4478.851 kb on - strand, within Pf6N2E2_3845 at 4479.254 kb on + strand, within Pf6N2E2_3845 at 4479.255 kb on - strand, within Pf6N2E2_3845 at 4479.255 kb on - strand, within Pf6N2E2_3845 at 4479.340 kb on + strand at 4479.341 kb on - strand at 4479.341 kb on - strand at 4479.341 kb on - strand at 4479.687 kb on + strand, within Pf6N2E2_3846 at 4479.687 kb on + strand, within Pf6N2E2_3846 at 4479.687 kb on + strand, within Pf6N2E2_3846 at 4479.688 kb on - strand, within Pf6N2E2_3846 at 4479.688 kb on - strand, within Pf6N2E2_3846 at 4479.688 kb on - strand, within Pf6N2E2_3846 at 4479.781 kb on - strand, within Pf6N2E2_3846 at 4479.781 kb on - strand, within Pf6N2E2_3846 at 4479.781 kb on - strand, within Pf6N2E2_3846 at 4479.783 kb on + strand, within Pf6N2E2_3846 at 4479.783 kb on + strand, within Pf6N2E2_3846 at 4479.783 kb on + strand, within Pf6N2E2_3846 at 4479.783 kb on + strand, within Pf6N2E2_3846 at 4479.784 kb on - strand, within Pf6N2E2_3846 at 4479.784 kb on - strand, within Pf6N2E2_3846 at 4479.784 kb on - strand, within Pf6N2E2_3846 at 4479.784 kb on - strand, within Pf6N2E2_3846 at 4479.784 kb on - strand, within Pf6N2E2_3846 at 4480.074 kb on + strand, within Pf6N2E2_3846 at 4480.074 kb on + strand, within Pf6N2E2_3846 at 4480.075 kb on - strand, within Pf6N2E2_3846 at 4480.219 kb on + strand, within Pf6N2E2_3846 at 4480.219 kb on + strand, within Pf6N2E2_3846 at 4480.220 kb on - strand, within Pf6N2E2_3846 at 4480.220 kb on - strand, within Pf6N2E2_3846 at 4480.506 kb on + strand at 4480.506 kb on + strand at 4480.506 kb on + strand at 4480.507 kb on - strand at 4480.507 kb on - strand at 4480.507 kb on - strand at 4480.507 kb on - strand at 4480.507 kb on - strand at 4480.508 kb on + strand at 4480.509 kb on - strand at 4480.509 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 4,476,383 + -0.2 4,476,395 - -0.7 4,476,455 + -0.5 4,476,456 - -1.3 4,476,456 - +0.6 4,476,476 - -0.4 4,476,522 + +0.2 4,476,555 + +0.1 4,476,742 - Pf6N2E2_3844 0.26 +0.3 4,476,875 + Pf6N2E2_3844 0.59 +1.0 4,476,875 + Pf6N2E2_3844 0.59 +0.4 4,477,036 + +0.1 4,477,036 + -0.6 4,477,037 - +0.0 4,477,037 - -0.5 4,477,037 - -0.2 4,477,037 - -0.4 4,477,037 - -0.4 4,477,040 + -0.4 4,477,041 - -0.9 4,477,041 - -0.4 4,477,251 + +0.0 4,477,251 + +0.5 4,477,252 - -0.8 4,477,264 + +0.0 4,477,266 + -1.1 4,477,267 - +0.5 4,477,267 - -1.0 4,477,267 - +0.8 4,477,294 + +0.1 4,477,294 + +0.2 4,477,294 + -0.2 4,477,294 + -0.3 4,477,294 + -0.1 4,477,295 - +0.4 4,477,295 - +0.3 4,477,295 - -0.1 4,477,340 + +0.2 4,477,340 + +1.3 4,477,341 - -0.1 4,477,341 - +0.4 4,477,411 + -1.0 4,477,411 + +0.0 4,477,412 - -0.2 4,477,412 - +0.3 4,477,412 - +1.1 4,477,412 - -0.5 4,477,599 - Pf6N2E2_3845 0.13 -0.2 4,477,619 + Pf6N2E2_3845 0.14 +0.4 4,477,856 - Pf6N2E2_3845 0.25 +0.1 4,477,856 - Pf6N2E2_3845 0.25 -0.3 4,477,856 - Pf6N2E2_3845 0.25 -0.5 4,477,988 + Pf6N2E2_3845 0.30 +0.2 4,477,989 - Pf6N2E2_3845 0.30 -0.3 4,478,038 + Pf6N2E2_3845 0.33 +0.8 4,478,038 + Pf6N2E2_3845 0.33 +1.1 4,478,039 - Pf6N2E2_3845 0.33 +0.2 4,478,601 + Pf6N2E2_3845 0.58 -0.0 4,478,601 + Pf6N2E2_3845 0.58 -0.0 4,478,602 - Pf6N2E2_3845 0.58 +0.0 4,478,602 - Pf6N2E2_3845 0.58 -0.3 4,478,803 + Pf6N2E2_3845 0.67 -1.6 4,478,803 + Pf6N2E2_3845 0.67 -1.4 4,478,804 - Pf6N2E2_3845 0.67 +0.2 4,478,804 - Pf6N2E2_3845 0.67 +0.0 4,478,804 - Pf6N2E2_3845 0.67 +0.0 4,478,851 - Pf6N2E2_3845 0.69 +0.6 4,478,851 - Pf6N2E2_3845 0.69 +0.8 4,479,254 + Pf6N2E2_3845 0.87 +0.4 4,479,255 - Pf6N2E2_3845 0.87 +0.0 4,479,255 - Pf6N2E2_3845 0.87 +0.6 4,479,340 + -0.4 4,479,341 - +0.5 4,479,341 - -0.5 4,479,341 - -0.5 4,479,687 + Pf6N2E2_3846 0.14 -0.1 4,479,687 + Pf6N2E2_3846 0.14 -0.5 4,479,687 + Pf6N2E2_3846 0.14 +0.0 4,479,688 - Pf6N2E2_3846 0.14 -1.2 4,479,688 - Pf6N2E2_3846 0.14 +0.4 4,479,688 - Pf6N2E2_3846 0.14 -0.1 4,479,781 - Pf6N2E2_3846 0.23 -0.7 4,479,781 - Pf6N2E2_3846 0.23 +0.2 4,479,781 - Pf6N2E2_3846 0.23 +0.3 4,479,783 + Pf6N2E2_3846 0.23 +0.3 4,479,783 + Pf6N2E2_3846 0.23 -0.0 4,479,783 + Pf6N2E2_3846 0.23 +0.7 4,479,783 + Pf6N2E2_3846 0.23 +0.4 4,479,784 - Pf6N2E2_3846 0.23 +0.0 4,479,784 - Pf6N2E2_3846 0.23 -0.6 4,479,784 - Pf6N2E2_3846 0.23 -0.4 4,479,784 - Pf6N2E2_3846 0.23 +0.3 4,479,784 - Pf6N2E2_3846 0.23 -0.0 4,480,074 + Pf6N2E2_3846 0.50 +0.1 4,480,074 + Pf6N2E2_3846 0.50 -2.4 4,480,075 - Pf6N2E2_3846 0.50 -0.1 4,480,219 + Pf6N2E2_3846 0.64 +1.4 4,480,219 + Pf6N2E2_3846 0.64 +1.1 4,480,220 - Pf6N2E2_3846 0.64 +0.3 4,480,220 - Pf6N2E2_3846 0.64 +0.7 4,480,506 + -0.7 4,480,506 + +0.3 4,480,506 + +0.0 4,480,507 - -0.8 4,480,507 - -1.4 4,480,507 - +0.2 4,480,507 - +0.9 4,480,507 - -0.3 4,480,508 + +1.2 4,480,509 - +0.5 4,480,509 - +0.4
Or see this region's nucleotide sequence