Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3659 and Pf6N2E2_3660 are separated by 221 nucleotides Pf6N2E2_3660 and Pf6N2E2_3661 are separated by 18 nucleotides Pf6N2E2_3661 and Pf6N2E2_3662 are separated by 192 nucleotides Pf6N2E2_3662 and Pf6N2E2_3663 are separated by 89 nucleotides
Pf6N2E2_3659 - Protein fixF, at 4,261,130 to 4,262,236
_3659
Pf6N2E2_3660 - Oligosaccharide repeat unit polymerase Wzy; O-antigen ligase, at 4,262,458 to 4,263,090
_3660
Pf6N2E2_3661 - hypothetical protein, at 4,263,109 to 4,263,969
_3661
Pf6N2E2_3662 - hypothetical protein, at 4,264,162 to 4,264,302
_3662
Pf6N2E2_3663 - Glycosyl transferase, at 4,264,392 to 4,265,294
_3663
Position (kb)
4263
4264 Strain fitness (log2 ratio)
-2
-1
0
1 at 4262.144 kb on - strand at 4262.144 kb on - strand at 4262.144 kb on - strand at 4262.147 kb on + strand at 4262.147 kb on + strand at 4262.147 kb on + strand at 4262.148 kb on - strand at 4262.148 kb on - strand at 4262.148 kb on - strand at 4262.148 kb on - strand at 4262.148 kb on - strand at 4262.148 kb on - strand at 4262.190 kb on + strand at 4262.190 kb on + strand at 4262.191 kb on - strand at 4262.191 kb on - strand at 4262.252 kb on + strand at 4262.264 kb on - strand at 4262.279 kb on + strand at 4262.279 kb on + strand at 4262.295 kb on - strand at 4262.376 kb on - strand at 4262.377 kb on + strand at 4262.377 kb on + strand at 4262.377 kb on + strand at 4262.412 kb on + strand at 4262.412 kb on + strand at 4262.417 kb on + strand at 4262.417 kb on + strand at 4263.097 kb on + strand at 4263.097 kb on + strand at 4263.097 kb on + strand at 4263.216 kb on - strand, within Pf6N2E2_3661 at 4263.216 kb on - strand, within Pf6N2E2_3661 at 4263.216 kb on - strand, within Pf6N2E2_3661 at 4263.493 kb on + strand, within Pf6N2E2_3661 at 4263.493 kb on + strand, within Pf6N2E2_3661 at 4263.494 kb on - strand, within Pf6N2E2_3661 at 4263.494 kb on - strand, within Pf6N2E2_3661 at 4263.494 kb on - strand, within Pf6N2E2_3661 at 4263.563 kb on - strand, within Pf6N2E2_3661 at 4263.601 kb on + strand, within Pf6N2E2_3661 at 4263.601 kb on + strand, within Pf6N2E2_3661 at 4263.601 kb on + strand, within Pf6N2E2_3661 at 4263.602 kb on - strand, within Pf6N2E2_3661 at 4263.608 kb on + strand, within Pf6N2E2_3661 at 4263.609 kb on - strand, within Pf6N2E2_3661 at 4263.609 kb on - strand, within Pf6N2E2_3661 at 4263.609 kb on - strand, within Pf6N2E2_3661 at 4263.702 kb on - strand, within Pf6N2E2_3661 at 4263.720 kb on - strand, within Pf6N2E2_3661 at 4263.806 kb on + strand, within Pf6N2E2_3661 at 4263.962 kb on + strand at 4263.963 kb on - strand at 4263.995 kb on + strand at 4263.998 kb on - strand at 4263.998 kb on - strand at 4264.426 kb on + strand at 4264.427 kb on - strand at 4264.427 kb on - strand at 4264.475 kb on - strand at 4264.475 kb on - strand at 4264.495 kb on + strand, within Pf6N2E2_3663 at 4264.496 kb on - strand, within Pf6N2E2_3663 at 4264.667 kb on + strand, within Pf6N2E2_3663 at 4264.668 kb on - strand, within Pf6N2E2_3663 at 4264.668 kb on - strand, within Pf6N2E2_3663 at 4264.669 kb on + strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.768 kb on + strand, within Pf6N2E2_3663 at 4264.844 kb on - strand, within Pf6N2E2_3663 at 4264.941 kb on - strand, within Pf6N2E2_3663
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 4,262,144 - -0.9 4,262,144 - -0.4 4,262,144 - -0.2 4,262,147 + +0.4 4,262,147 + -0.9 4,262,147 + -0.2 4,262,148 - +1.2 4,262,148 - -0.0 4,262,148 - -0.4 4,262,148 - +0.1 4,262,148 - -2.1 4,262,148 - +0.3 4,262,190 + -1.2 4,262,190 + -1.3 4,262,191 - +0.4 4,262,191 - -2.0 4,262,252 + -0.4 4,262,264 - -0.9 4,262,279 + +0.2 4,262,279 + -0.8 4,262,295 - -0.9 4,262,376 - +0.4 4,262,377 + +0.1 4,262,377 + -2.2 4,262,377 + -1.2 4,262,412 + +0.6 4,262,412 + +1.2 4,262,417 + -0.1 4,262,417 + -0.7 4,263,097 + -0.0 4,263,097 + +0.9 4,263,097 + +1.3 4,263,216 - Pf6N2E2_3661 0.12 +0.8 4,263,216 - Pf6N2E2_3661 0.12 +1.2 4,263,216 - Pf6N2E2_3661 0.12 -1.9 4,263,493 + Pf6N2E2_3661 0.45 +1.4 4,263,493 + Pf6N2E2_3661 0.45 -0.3 4,263,494 - Pf6N2E2_3661 0.45 -0.5 4,263,494 - Pf6N2E2_3661 0.45 -1.6 4,263,494 - Pf6N2E2_3661 0.45 -1.4 4,263,563 - Pf6N2E2_3661 0.53 -0.2 4,263,601 + Pf6N2E2_3661 0.57 -0.9 4,263,601 + Pf6N2E2_3661 0.57 -0.3 4,263,601 + Pf6N2E2_3661 0.57 -1.6 4,263,602 - Pf6N2E2_3661 0.57 -0.6 4,263,608 + Pf6N2E2_3661 0.58 -1.6 4,263,609 - Pf6N2E2_3661 0.58 -1.0 4,263,609 - Pf6N2E2_3661 0.58 -0.8 4,263,609 - Pf6N2E2_3661 0.58 -0.7 4,263,702 - Pf6N2E2_3661 0.69 -0.8 4,263,720 - Pf6N2E2_3661 0.71 -1.0 4,263,806 + Pf6N2E2_3661 0.81 +0.8 4,263,962 + -2.5 4,263,963 - +0.4 4,263,995 + -1.0 4,263,998 - +0.6 4,263,998 - -0.5 4,264,426 + -1.5 4,264,427 - +1.2 4,264,427 - -1.1 4,264,475 - -0.4 4,264,475 - +1.0 4,264,495 + Pf6N2E2_3663 0.11 -1.5 4,264,496 - Pf6N2E2_3663 0.12 +0.8 4,264,667 + Pf6N2E2_3663 0.30 +0.6 4,264,668 - Pf6N2E2_3663 0.31 -0.7 4,264,668 - Pf6N2E2_3663 0.31 -1.3 4,264,669 + Pf6N2E2_3663 0.31 -0.3 4,264,670 - Pf6N2E2_3663 0.31 +1.0 4,264,670 - Pf6N2E2_3663 0.31 -0.0 4,264,670 - Pf6N2E2_3663 0.31 +1.1 4,264,670 - Pf6N2E2_3663 0.31 -0.2 4,264,670 - Pf6N2E2_3663 0.31 -0.0 4,264,670 - Pf6N2E2_3663 0.31 -0.7 4,264,768 + Pf6N2E2_3663 0.42 -1.1 4,264,844 - Pf6N2E2_3663 0.50 +0.7 4,264,941 - Pf6N2E2_3663 0.61 -0.0
Or see this region's nucleotide sequence