Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2883 and Pf6N2E2_2884 are separated by 17 nucleotides Pf6N2E2_2884 and Pf6N2E2_2885 overlap by 14 nucleotides Pf6N2E2_2885 and Pf6N2E2_2886 are separated by 187 nucleotides
Pf6N2E2_2883 - 4-Hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) @ 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14), at 3,370,935 to 3,371,600
_2883
Pf6N2E2_2884 - 6-phosphogluconolactonase (EC 3.1.1.31), eukaryotic type, at 3,371,618 to 3,372,331
_2884
Pf6N2E2_2885 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 3,372,318 to 3,373,787
_2885
Pf6N2E2_2886 - Phosphogluconate repressor HexR, RpiR family, at 3,373,975 to 3,374,844
_2886
Position (kb)
3372
3373
3374 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3371.632 kb on - strand at 3371.632 kb on - strand at 3371.632 kb on - strand at 3371.645 kb on - strand at 3371.645 kb on - strand at 3372.332 kb on - strand at 3372.533 kb on - strand, within Pf6N2E2_2885 at 3372.560 kb on - strand, within Pf6N2E2_2885 at 3372.722 kb on - strand, within Pf6N2E2_2885 at 3372.722 kb on - strand, within Pf6N2E2_2885 at 3372.932 kb on - strand, within Pf6N2E2_2885 at 3372.932 kb on - strand, within Pf6N2E2_2885 at 3372.932 kb on - strand, within Pf6N2E2_2885 at 3372.932 kb on - strand, within Pf6N2E2_2885 at 3373.181 kb on - strand, within Pf6N2E2_2885 at 3373.262 kb on - strand, within Pf6N2E2_2885 at 3373.277 kb on - strand, within Pf6N2E2_2885 at 3373.277 kb on - strand, within Pf6N2E2_2885 at 3373.281 kb on - strand, within Pf6N2E2_2885 at 3373.281 kb on - strand, within Pf6N2E2_2885 at 3373.421 kb on - strand, within Pf6N2E2_2885 at 3373.421 kb on - strand, within Pf6N2E2_2885 at 3373.445 kb on - strand, within Pf6N2E2_2885 at 3373.586 kb on - strand, within Pf6N2E2_2885 at 3373.586 kb on - strand, within Pf6N2E2_2885 at 3373.586 kb on - strand, within Pf6N2E2_2885 at 3373.586 kb on - strand, within Pf6N2E2_2885 at 3373.837 kb on - strand at 3373.859 kb on - strand at 3373.862 kb on - strand at 3373.920 kb on - strand at 3373.920 kb on - strand at 3373.925 kb on - strand at 3373.945 kb on - strand at 3373.963 kb on + strand at 3373.964 kb on - strand at 3373.974 kb on + strand at 3373.974 kb on + strand at 3373.974 kb on + strand at 3373.974 kb on + strand at 3373.975 kb on - strand at 3373.975 kb on - strand at 3373.975 kb on - strand at 3373.975 kb on - strand at 3374.224 kb on + strand, within Pf6N2E2_2886 at 3374.224 kb on + strand, within Pf6N2E2_2886 at 3374.224 kb on + strand, within Pf6N2E2_2886 at 3374.224 kb on + strand, within Pf6N2E2_2886 at 3374.224 kb on + strand, within Pf6N2E2_2886 at 3374.224 kb on + strand, within Pf6N2E2_2886 at 3374.225 kb on - strand, within Pf6N2E2_2886 at 3374.225 kb on - strand, within Pf6N2E2_2886 at 3374.225 kb on - strand, within Pf6N2E2_2886 at 3374.266 kb on + strand, within Pf6N2E2_2886 at 3374.266 kb on + strand, within Pf6N2E2_2886 at 3374.266 kb on + strand, within Pf6N2E2_2886 at 3374.266 kb on + strand, within Pf6N2E2_2886 at 3374.266 kb on + strand, within Pf6N2E2_2886 at 3374.267 kb on - strand, within Pf6N2E2_2886 at 3374.267 kb on - strand, within Pf6N2E2_2886 at 3374.474 kb on + strand, within Pf6N2E2_2886 at 3374.474 kb on + strand, within Pf6N2E2_2886 at 3374.474 kb on + strand, within Pf6N2E2_2886 at 3374.475 kb on - strand, within Pf6N2E2_2886 at 3374.475 kb on - strand, within Pf6N2E2_2886 at 3374.475 kb on - strand, within Pf6N2E2_2886 at 3374.475 kb on - strand, within Pf6N2E2_2886 at 3374.475 kb on - strand, within Pf6N2E2_2886 at 3374.682 kb on + strand, within Pf6N2E2_2886 at 3374.682 kb on + strand, within Pf6N2E2_2886 at 3374.682 kb on + strand, within Pf6N2E2_2886 at 3374.682 kb on + strand, within Pf6N2E2_2886 at 3374.682 kb on + strand, within Pf6N2E2_2886 at 3374.682 kb on + strand, within Pf6N2E2_2886 at 3374.682 kb on + strand, within Pf6N2E2_2886 at 3374.682 kb on + strand, within Pf6N2E2_2886 at 3374.682 kb on + strand, within Pf6N2E2_2886 at 3374.683 kb on - strand, within Pf6N2E2_2886 at 3374.683 kb on - strand, within Pf6N2E2_2886 at 3374.683 kb on - strand, within Pf6N2E2_2886 at 3374.683 kb on - strand, within Pf6N2E2_2886 at 3374.683 kb on - strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.686 kb on + strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.687 kb on - strand, within Pf6N2E2_2886 at 3374.688 kb on + strand, within Pf6N2E2_2886 at 3374.688 kb on + strand, within Pf6N2E2_2886 at 3374.688 kb on + strand, within Pf6N2E2_2886 at 3374.688 kb on + strand, within Pf6N2E2_2886 at 3374.689 kb on - strand, within Pf6N2E2_2886 at 3374.689 kb on - strand, within Pf6N2E2_2886
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 3,371,632 - -0.2 3,371,632 - -0.6 3,371,632 - -1.0 3,371,645 - +1.4 3,371,645 - -2.8 3,372,332 - -0.3 3,372,533 - Pf6N2E2_2885 0.15 -1.9 3,372,560 - Pf6N2E2_2885 0.16 -1.0 3,372,722 - Pf6N2E2_2885 0.27 -0.7 3,372,722 - Pf6N2E2_2885 0.27 -2.6 3,372,932 - Pf6N2E2_2885 0.42 -0.6 3,372,932 - Pf6N2E2_2885 0.42 -0.7 3,372,932 - Pf6N2E2_2885 0.42 -0.5 3,372,932 - Pf6N2E2_2885 0.42 -1.6 3,373,181 - Pf6N2E2_2885 0.59 -0.3 3,373,262 - Pf6N2E2_2885 0.64 -0.3 3,373,277 - Pf6N2E2_2885 0.65 -0.3 3,373,277 - Pf6N2E2_2885 0.65 -0.5 3,373,281 - Pf6N2E2_2885 0.66 -1.2 3,373,281 - Pf6N2E2_2885 0.66 +0.3 3,373,421 - Pf6N2E2_2885 0.75 -0.3 3,373,421 - Pf6N2E2_2885 0.75 +1.0 3,373,445 - Pf6N2E2_2885 0.77 -2.0 3,373,586 - Pf6N2E2_2885 0.86 -0.1 3,373,586 - Pf6N2E2_2885 0.86 -0.9 3,373,586 - Pf6N2E2_2885 0.86 -0.8 3,373,586 - Pf6N2E2_2885 0.86 -1.1 3,373,837 - +0.1 3,373,859 - -0.7 3,373,862 - -0.4 3,373,920 - -0.6 3,373,920 - +0.9 3,373,925 - +0.4 3,373,945 - +0.1 3,373,963 + -0.6 3,373,964 - +0.3 3,373,974 + -0.7 3,373,974 + -0.2 3,373,974 + +0.5 3,373,974 + +0.5 3,373,975 - +0.8 3,373,975 - +0.7 3,373,975 - -0.1 3,373,975 - +0.5 3,374,224 + Pf6N2E2_2886 0.29 +0.7 3,374,224 + Pf6N2E2_2886 0.29 +0.3 3,374,224 + Pf6N2E2_2886 0.29 +0.9 3,374,224 + Pf6N2E2_2886 0.29 +1.9 3,374,224 + Pf6N2E2_2886 0.29 +0.5 3,374,224 + Pf6N2E2_2886 0.29 +1.1 3,374,225 - Pf6N2E2_2886 0.29 +0.3 3,374,225 - Pf6N2E2_2886 0.29 -0.2 3,374,225 - Pf6N2E2_2886 0.29 +0.3 3,374,266 + Pf6N2E2_2886 0.33 -1.0 3,374,266 + Pf6N2E2_2886 0.33 -1.1 3,374,266 + Pf6N2E2_2886 0.33 +0.5 3,374,266 + Pf6N2E2_2886 0.33 -0.2 3,374,266 + Pf6N2E2_2886 0.33 +0.6 3,374,267 - Pf6N2E2_2886 0.34 +0.3 3,374,267 - Pf6N2E2_2886 0.34 -0.4 3,374,474 + Pf6N2E2_2886 0.57 +0.7 3,374,474 + Pf6N2E2_2886 0.57 -0.3 3,374,474 + Pf6N2E2_2886 0.57 +0.5 3,374,475 - Pf6N2E2_2886 0.57 -0.0 3,374,475 - Pf6N2E2_2886 0.57 +0.1 3,374,475 - Pf6N2E2_2886 0.57 -0.6 3,374,475 - Pf6N2E2_2886 0.57 -0.6 3,374,475 - Pf6N2E2_2886 0.57 -0.0 3,374,682 + Pf6N2E2_2886 0.81 +0.5 3,374,682 + Pf6N2E2_2886 0.81 +0.7 3,374,682 + Pf6N2E2_2886 0.81 -0.4 3,374,682 + Pf6N2E2_2886 0.81 +1.0 3,374,682 + Pf6N2E2_2886 0.81 +0.2 3,374,682 + Pf6N2E2_2886 0.81 +1.3 3,374,682 + Pf6N2E2_2886 0.81 +0.5 3,374,682 + Pf6N2E2_2886 0.81 +0.6 3,374,682 + Pf6N2E2_2886 0.81 +0.2 3,374,683 - Pf6N2E2_2886 0.81 +0.2 3,374,683 - Pf6N2E2_2886 0.81 +0.9 3,374,683 - Pf6N2E2_2886 0.81 +0.5 3,374,683 - Pf6N2E2_2886 0.81 +0.6 3,374,683 - Pf6N2E2_2886 0.81 -0.2 3,374,686 + Pf6N2E2_2886 0.82 -0.5 3,374,686 + Pf6N2E2_2886 0.82 -0.5 3,374,686 + Pf6N2E2_2886 0.82 +0.4 3,374,686 + Pf6N2E2_2886 0.82 +1.1 3,374,686 + Pf6N2E2_2886 0.82 +1.0 3,374,686 + Pf6N2E2_2886 0.82 -0.3 3,374,686 + Pf6N2E2_2886 0.82 +0.4 3,374,686 + Pf6N2E2_2886 0.82 +0.5 3,374,686 + Pf6N2E2_2886 0.82 +0.6 3,374,686 + Pf6N2E2_2886 0.82 -0.0 3,374,686 + Pf6N2E2_2886 0.82 +0.4 3,374,686 + Pf6N2E2_2886 0.82 +0.1 3,374,686 + Pf6N2E2_2886 0.82 +0.5 3,374,686 + Pf6N2E2_2886 0.82 -0.2 3,374,686 + Pf6N2E2_2886 0.82 +0.1 3,374,686 + Pf6N2E2_2886 0.82 +0.2 3,374,686 + Pf6N2E2_2886 0.82 -0.1 3,374,686 + Pf6N2E2_2886 0.82 -0.5 3,374,686 + Pf6N2E2_2886 0.82 +1.3 3,374,686 + Pf6N2E2_2886 0.82 +0.5 3,374,686 + Pf6N2E2_2886 0.82 -0.4 3,374,686 + Pf6N2E2_2886 0.82 +0.6 3,374,686 + Pf6N2E2_2886 0.82 +1.2 3,374,686 + Pf6N2E2_2886 0.82 +0.0 3,374,686 + Pf6N2E2_2886 0.82 +0.3 3,374,686 + Pf6N2E2_2886 0.82 +0.4 3,374,686 + Pf6N2E2_2886 0.82 +0.2 3,374,686 + Pf6N2E2_2886 0.82 +0.4 3,374,686 + Pf6N2E2_2886 0.82 +1.4 3,374,686 + Pf6N2E2_2886 0.82 +1.2 3,374,687 - Pf6N2E2_2886 0.82 +0.4 3,374,687 - Pf6N2E2_2886 0.82 +0.9 3,374,687 - Pf6N2E2_2886 0.82 +0.4 3,374,687 - +0.7 3,374,687 - Pf6N2E2_2886 0.82 -0.9 3,374,687 - Pf6N2E2_2886 0.82 -0.4 3,374,687 - Pf6N2E2_2886 0.82 +0.6 3,374,687 - Pf6N2E2_2886 0.82 +0.6 3,374,687 - Pf6N2E2_2886 0.82 -1.0 3,374,687 - Pf6N2E2_2886 0.82 -0.1 3,374,687 - Pf6N2E2_2886 0.82 -0.1 3,374,687 - Pf6N2E2_2886 0.82 -0.4 3,374,687 - Pf6N2E2_2886 0.82 +0.4 3,374,687 - Pf6N2E2_2886 0.82 -0.5 3,374,687 - Pf6N2E2_2886 0.82 +0.3 3,374,687 - Pf6N2E2_2886 0.82 +0.4 3,374,687 - Pf6N2E2_2886 0.82 +0.9 3,374,687 - Pf6N2E2_2886 0.82 +1.0 3,374,687 - Pf6N2E2_2886 0.82 -0.7 3,374,687 - Pf6N2E2_2886 0.82 -0.9 3,374,687 - Pf6N2E2_2886 0.82 +0.3 3,374,687 - Pf6N2E2_2886 0.82 +0.1 3,374,687 - Pf6N2E2_2886 0.82 -0.2 3,374,687 - Pf6N2E2_2886 0.82 -0.1 3,374,687 - Pf6N2E2_2886 0.82 +0.2 3,374,687 - Pf6N2E2_2886 0.82 +0.3 3,374,687 - Pf6N2E2_2886 0.82 +0.9 3,374,687 - Pf6N2E2_2886 0.82 +0.4 3,374,687 - Pf6N2E2_2886 0.82 +0.4 3,374,687 - Pf6N2E2_2886 0.82 +0.1 3,374,687 - Pf6N2E2_2886 0.82 +0.5 3,374,688 + Pf6N2E2_2886 0.82 +0.2 3,374,688 + Pf6N2E2_2886 0.82 -0.0 3,374,688 + Pf6N2E2_2886 0.82 +0.8 3,374,688 + Pf6N2E2_2886 0.82 +0.7 3,374,689 - Pf6N2E2_2886 0.82 +0.1 3,374,689 - Pf6N2E2_2886 0.82 +0.3
Or see this region's nucleotide sequence