Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2525 and Pf6N2E2_2526 are separated by 4 nucleotides Pf6N2E2_2526 and Pf6N2E2_2527 are separated by 167 nucleotides Pf6N2E2_2527 and Pf6N2E2_2528 are separated by 353 nucleotides
Pf6N2E2_2525 - 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64), at 2,982,418 to 2,983,116
_2525
Pf6N2E2_2526 - Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase, at 2,983,121 to 2,983,792
_2526
Pf6N2E2_2527 - Oxidoreductase, short chain dehydrogenase/reductase family, at 2,983,960 to 2,984,700
_2527
Pf6N2E2_2528 - GGDEF domain protein, at 2,985,054 to 2,985,980
_2528
Position (kb)
2983
2984
2985 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2983.114 kb on + strand at 2983.114 kb on + strand at 2983.115 kb on - strand at 2983.115 kb on - strand at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.683 kb on - strand, within Pf6N2E2_2526 at 2983.683 kb on - strand, within Pf6N2E2_2526 at 2983.688 kb on + strand, within Pf6N2E2_2526 at 2983.689 kb on - strand, within Pf6N2E2_2526 at 2983.942 kb on + strand at 2983.942 kb on + strand at 2983.942 kb on + strand at 2983.959 kb on + strand at 2983.959 kb on + strand at 2983.960 kb on - strand at 2984.057 kb on - strand, within Pf6N2E2_2527 at 2984.119 kb on + strand, within Pf6N2E2_2527 at 2984.119 kb on + strand, within Pf6N2E2_2527 at 2984.197 kb on + strand, within Pf6N2E2_2527 at 2984.197 kb on + strand, within Pf6N2E2_2527 at 2984.198 kb on - strand, within Pf6N2E2_2527 at 2984.198 kb on - strand, within Pf6N2E2_2527 at 2984.280 kb on + strand, within Pf6N2E2_2527 at 2984.355 kb on + strand, within Pf6N2E2_2527 at 2984.444 kb on - strand, within Pf6N2E2_2527 at 2984.455 kb on + strand, within Pf6N2E2_2527 at 2984.644 kb on + strand at 2984.727 kb on + strand at 2984.727 kb on + strand at 2984.728 kb on - strand at 2984.794 kb on + strand at 2984.795 kb on - strand at 2984.836 kb on + strand at 2984.902 kb on + strand at 2984.903 kb on - strand at 2984.903 kb on - strand at 2984.924 kb on + strand at 2984.924 kb on + strand at 2984.925 kb on - strand at 2984.925 kb on - strand at 2984.925 kb on - strand at 2984.925 kb on - strand at 2984.925 kb on - strand at 2984.985 kb on + strand at 2984.985 kb on + strand at 2984.986 kb on - strand at 2985.033 kb on + strand at 2985.033 kb on + strand at 2985.033 kb on + strand at 2985.034 kb on - strand at 2985.034 kb on - strand at 2985.034 kb on - strand at 2985.034 kb on - strand at 2985.034 kb on - strand at 2985.034 kb on - strand at 2985.034 kb on - strand at 2985.054 kb on - strand at 2985.274 kb on - strand, within Pf6N2E2_2528 at 2985.336 kb on + strand, within Pf6N2E2_2528 at 2985.336 kb on + strand, within Pf6N2E2_2528 at 2985.336 kb on + strand, within Pf6N2E2_2528 at 2985.336 kb on + strand, within Pf6N2E2_2528 at 2985.336 kb on + strand, within Pf6N2E2_2528 at 2985.336 kb on + strand, within Pf6N2E2_2528 at 2985.336 kb on + strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.339 kb on - strand, within Pf6N2E2_2528
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 2,983,114 + +0.4 2,983,114 + -1.0 2,983,115 - -1.3 2,983,115 - -1.6 2,983,682 + Pf6N2E2_2526 0.83 -2.0 2,983,682 + Pf6N2E2_2526 0.83 -1.0 2,983,682 + Pf6N2E2_2526 0.83 -2.0 2,983,682 + Pf6N2E2_2526 0.83 -2.3 2,983,682 + Pf6N2E2_2526 0.83 -1.5 2,983,682 + Pf6N2E2_2526 0.83 -3.2 2,983,683 - Pf6N2E2_2526 0.84 -0.9 2,983,683 - Pf6N2E2_2526 0.84 -3.5 2,983,688 + Pf6N2E2_2526 0.84 -2.1 2,983,689 - Pf6N2E2_2526 0.85 -1.2 2,983,942 + -1.8 2,983,942 + -0.9 2,983,942 + -0.9 2,983,959 + -0.5 2,983,959 + -0.3 2,983,960 - -1.5 2,984,057 - Pf6N2E2_2527 0.13 -1.7 2,984,119 + Pf6N2E2_2527 0.21 -3.7 2,984,119 + Pf6N2E2_2527 0.21 -1.4 2,984,197 + Pf6N2E2_2527 0.32 -1.7 2,984,197 + Pf6N2E2_2527 0.32 -0.6 2,984,198 - Pf6N2E2_2527 0.32 -1.7 2,984,198 - Pf6N2E2_2527 0.32 -1.1 2,984,280 + Pf6N2E2_2527 0.43 -0.9 2,984,355 + Pf6N2E2_2527 0.53 -0.6 2,984,444 - Pf6N2E2_2527 0.65 -0.8 2,984,455 + Pf6N2E2_2527 0.67 -1.2 2,984,644 + -0.1 2,984,727 + -0.2 2,984,727 + -1.3 2,984,728 - +0.3 2,984,794 + +1.1 2,984,795 - -0.7 2,984,836 + +0.9 2,984,902 + -0.5 2,984,903 - +0.5 2,984,903 - +0.5 2,984,924 + +0.6 2,984,924 + -0.1 2,984,925 - +0.9 2,984,925 - +0.7 2,984,925 - +0.0 2,984,925 - +0.2 2,984,925 - +0.2 2,984,985 + -0.6 2,984,985 + -0.5 2,984,986 - +1.0 2,985,033 + -1.6 2,985,033 + +0.6 2,985,033 + +0.2 2,985,034 - +0.6 2,985,034 - -1.5 2,985,034 - -0.2 2,985,034 - +1.1 2,985,034 - +0.1 2,985,034 - -0.7 2,985,034 - -0.1 2,985,054 - +1.3 2,985,274 - Pf6N2E2_2528 0.24 +0.3 2,985,336 + Pf6N2E2_2528 0.30 +1.1 2,985,336 + Pf6N2E2_2528 0.30 -1.1 2,985,336 + Pf6N2E2_2528 0.30 +0.6 2,985,336 + Pf6N2E2_2528 0.30 +1.1 2,985,336 + Pf6N2E2_2528 0.30 +1.5 2,985,336 + Pf6N2E2_2528 0.30 +0.3 2,985,336 + Pf6N2E2_2528 0.30 +0.3 2,985,337 - Pf6N2E2_2528 0.31 +0.9 2,985,337 - Pf6N2E2_2528 0.31 +0.7 2,985,337 - Pf6N2E2_2528 0.31 +0.4 2,985,337 - Pf6N2E2_2528 0.31 -0.6 2,985,337 - Pf6N2E2_2528 0.31 -1.3 2,985,337 - Pf6N2E2_2528 0.31 -1.3 2,985,337 - Pf6N2E2_2528 0.31 -1.3 2,985,337 - Pf6N2E2_2528 0.31 +0.3 2,985,337 - Pf6N2E2_2528 0.31 -0.6 2,985,339 - Pf6N2E2_2528 0.31 -0.6
Or see this region's nucleotide sequence