Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2493 and Pf6N2E2_2494 are separated by 10 nucleotides Pf6N2E2_2494 and Pf6N2E2_2495 are separated by 490 nucleotides
Pf6N2E2_2493 - Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 2,940,552 to 2,942,606
_2493
Pf6N2E2_2494 - Potassium-transporting ATPase A chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 2,942,617 to 2,944,311
_2494
Pf6N2E2_2495 - Ethanolamine permease, at 2,944,802 to 2,946,166
_2495
Position (kb)
2942
2943
2944
2945 Strain fitness (log2 ratio)
-1
0
1 at 2941.627 kb on + strand, within Pf6N2E2_2493 at 2941.627 kb on + strand, within Pf6N2E2_2493 at 2941.628 kb on - strand, within Pf6N2E2_2493 at 2941.628 kb on - strand, within Pf6N2E2_2493 at 2941.628 kb on - strand, within Pf6N2E2_2493 at 2941.637 kb on + strand, within Pf6N2E2_2493 at 2942.057 kb on + strand, within Pf6N2E2_2493 at 2942.600 kb on + strand at 2942.600 kb on + strand at 2942.600 kb on + strand at 2942.600 kb on + strand at 2942.601 kb on - strand at 2942.601 kb on - strand at 2942.601 kb on - strand at 2942.921 kb on + strand, within Pf6N2E2_2494 at 2942.921 kb on + strand, within Pf6N2E2_2494 at 2942.921 kb on + strand, within Pf6N2E2_2494 at 2942.921 kb on + strand, within Pf6N2E2_2494 at 2942.921 kb on + strand, within Pf6N2E2_2494 at 2942.921 kb on + strand, within Pf6N2E2_2494 at 2942.922 kb on - strand, within Pf6N2E2_2494 at 2942.922 kb on - strand, within Pf6N2E2_2494 at 2942.922 kb on - strand, within Pf6N2E2_2494 at 2942.927 kb on + strand, within Pf6N2E2_2494 at 2942.928 kb on - strand, within Pf6N2E2_2494 at 2942.928 kb on - strand, within Pf6N2E2_2494 at 2943.090 kb on - strand, within Pf6N2E2_2494 at 2943.139 kb on + strand, within Pf6N2E2_2494 at 2943.139 kb on + strand, within Pf6N2E2_2494 at 2943.482 kb on + strand, within Pf6N2E2_2494 at 2943.482 kb on + strand, within Pf6N2E2_2494 at 2943.483 kb on - strand, within Pf6N2E2_2494 at 2943.483 kb on - strand, within Pf6N2E2_2494 at 2943.483 kb on - strand, within Pf6N2E2_2494 at 2943.483 kb on - strand, within Pf6N2E2_2494 at 2943.698 kb on + strand, within Pf6N2E2_2494 at 2943.699 kb on - strand, within Pf6N2E2_2494 at 2943.699 kb on - strand, within Pf6N2E2_2494 at 2943.923 kb on + strand, within Pf6N2E2_2494 at 2943.924 kb on - strand, within Pf6N2E2_2494 at 2943.947 kb on + strand, within Pf6N2E2_2494 at 2943.947 kb on + strand, within Pf6N2E2_2494 at 2943.947 kb on + strand, within Pf6N2E2_2494 at 2943.947 kb on + strand, within Pf6N2E2_2494 at 2943.947 kb on + strand, within Pf6N2E2_2494 at 2944.029 kb on + strand, within Pf6N2E2_2494 at 2944.030 kb on - strand, within Pf6N2E2_2494 at 2944.116 kb on - strand, within Pf6N2E2_2494 at 2944.116 kb on - strand, within Pf6N2E2_2494 at 2944.230 kb on - strand at 2944.232 kb on + strand at 2944.232 kb on + strand at 2944.233 kb on - strand at 2944.295 kb on + strand at 2944.295 kb on + strand at 2944.311 kb on + strand at 2944.321 kb on + strand at 2944.321 kb on + strand at 2944.322 kb on - strand at 2944.322 kb on - strand at 2944.357 kb on + strand at 2944.357 kb on + strand at 2944.723 kb on - strand at 2944.781 kb on - strand at 2944.843 kb on - strand at 2944.895 kb on + strand at 2944.896 kb on - strand at 2944.896 kb on - strand at 2944.896 kb on - strand at 2944.896 kb on - strand at 2944.958 kb on + strand, within Pf6N2E2_2495 at 2944.958 kb on + strand, within Pf6N2E2_2495 at 2944.958 kb on + strand, within Pf6N2E2_2495 at 2944.958 kb on + strand, within Pf6N2E2_2495 at 2944.959 kb on - strand, within Pf6N2E2_2495 at 2944.959 kb on - strand, within Pf6N2E2_2495 at 2944.959 kb on - strand, within Pf6N2E2_2495 at 2944.959 kb on - strand, within Pf6N2E2_2495 at 2944.959 kb on - strand, within Pf6N2E2_2495 at 2945.027 kb on + strand, within Pf6N2E2_2495 at 2945.028 kb on - strand, within Pf6N2E2_2495 at 2945.186 kb on + strand, within Pf6N2E2_2495 at 2945.186 kb on + strand, within Pf6N2E2_2495 at 2945.187 kb on - strand, within Pf6N2E2_2495 at 2945.187 kb on - strand, within Pf6N2E2_2495 at 2945.187 kb on - strand, within Pf6N2E2_2495
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 2,941,627 + Pf6N2E2_2493 0.52 +0.2 2,941,627 + Pf6N2E2_2493 0.52 +1.2 2,941,628 - Pf6N2E2_2493 0.52 +0.3 2,941,628 - Pf6N2E2_2493 0.52 -0.1 2,941,628 - Pf6N2E2_2493 0.52 +0.7 2,941,637 + Pf6N2E2_2493 0.53 -0.4 2,942,057 + Pf6N2E2_2493 0.73 +0.0 2,942,600 + -0.0 2,942,600 + -1.0 2,942,600 + +1.0 2,942,600 + +1.5 2,942,601 - +1.2 2,942,601 - -0.4 2,942,601 - -0.0 2,942,921 + Pf6N2E2_2494 0.18 +0.0 2,942,921 + Pf6N2E2_2494 0.18 +0.4 2,942,921 + Pf6N2E2_2494 0.18 +0.0 2,942,921 + Pf6N2E2_2494 0.18 -0.4 2,942,921 + Pf6N2E2_2494 0.18 -1.1 2,942,921 + Pf6N2E2_2494 0.18 -0.4 2,942,922 - Pf6N2E2_2494 0.18 +0.6 2,942,922 - Pf6N2E2_2494 0.18 +0.4 2,942,922 - Pf6N2E2_2494 0.18 +0.9 2,942,927 + Pf6N2E2_2494 0.18 +0.3 2,942,928 - Pf6N2E2_2494 0.18 +0.2 2,942,928 - Pf6N2E2_2494 0.18 +0.9 2,943,090 - Pf6N2E2_2494 0.28 -0.4 2,943,139 + Pf6N2E2_2494 0.31 +0.6 2,943,139 + Pf6N2E2_2494 0.31 -0.4 2,943,482 + Pf6N2E2_2494 0.51 +0.7 2,943,482 + Pf6N2E2_2494 0.51 +0.7 2,943,483 - Pf6N2E2_2494 0.51 +0.3 2,943,483 - Pf6N2E2_2494 0.51 -0.8 2,943,483 - Pf6N2E2_2494 0.51 -0.2 2,943,483 - Pf6N2E2_2494 0.51 +0.3 2,943,698 + Pf6N2E2_2494 0.64 +0.0 2,943,699 - Pf6N2E2_2494 0.64 +0.2 2,943,699 - Pf6N2E2_2494 0.64 -0.7 2,943,923 + Pf6N2E2_2494 0.77 -0.4 2,943,924 - Pf6N2E2_2494 0.77 +0.9 2,943,947 + Pf6N2E2_2494 0.78 -1.0 2,943,947 + Pf6N2E2_2494 0.78 +0.4 2,943,947 + Pf6N2E2_2494 0.78 +0.0 2,943,947 + Pf6N2E2_2494 0.78 +0.1 2,943,947 + Pf6N2E2_2494 0.78 +1.0 2,944,029 + Pf6N2E2_2494 0.83 +1.2 2,944,030 - Pf6N2E2_2494 0.83 +0.9 2,944,116 - Pf6N2E2_2494 0.88 -0.2 2,944,116 - Pf6N2E2_2494 0.88 -0.3 2,944,230 - +0.2 2,944,232 + -0.3 2,944,232 + +0.9 2,944,233 - -0.5 2,944,295 + +0.6 2,944,295 + +1.3 2,944,311 + -0.9 2,944,321 + -1.0 2,944,321 + +0.9 2,944,322 - +0.7 2,944,322 - +0.4 2,944,357 + -0.8 2,944,357 + -0.1 2,944,723 - -0.5 2,944,781 - -0.2 2,944,843 - +0.2 2,944,895 + +0.1 2,944,896 - +0.7 2,944,896 - +0.6 2,944,896 - -0.7 2,944,896 - -0.7 2,944,958 + Pf6N2E2_2495 0.11 -0.1 2,944,958 + Pf6N2E2_2495 0.11 +0.5 2,944,958 + Pf6N2E2_2495 0.11 -0.2 2,944,958 + Pf6N2E2_2495 0.11 -0.1 2,944,959 - Pf6N2E2_2495 0.12 +0.4 2,944,959 - Pf6N2E2_2495 0.12 +0.7 2,944,959 - Pf6N2E2_2495 0.12 +0.6 2,944,959 - Pf6N2E2_2495 0.12 +0.9 2,944,959 - Pf6N2E2_2495 0.12 +0.2 2,945,027 + Pf6N2E2_2495 0.16 -0.9 2,945,028 - Pf6N2E2_2495 0.17 -1.1 2,945,186 + Pf6N2E2_2495 0.28 -0.6 2,945,186 + Pf6N2E2_2495 0.28 +0.7 2,945,187 - Pf6N2E2_2495 0.28 +0.1 2,945,187 - Pf6N2E2_2495 0.28 -0.1 2,945,187 - Pf6N2E2_2495 0.28 +0.3
Or see this region's nucleotide sequence