Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2419 and Pf6N2E2_2420 are separated by 30 nucleotides Pf6N2E2_2420 and Pf6N2E2_2421 are separated by 263 nucleotides Pf6N2E2_2421 and Pf6N2E2_2422 are separated by 59 nucleotides Pf6N2E2_2422 and Pf6N2E2_2423 overlap by 17 nucleotides Pf6N2E2_2423 and Pf6N2E2_2424 are separated by 78 nucleotides
Pf6N2E2_2419 - Transcription regulator [contains diacylglycerol kinase catalytic domain], at 2,871,202 to 2,872,119
_2419
Pf6N2E2_2420 - hypothetical protein, at 2,872,150 to 2,872,317
_2420
Pf6N2E2_2421 - hypothetical protein, at 2,872,581 to 2,872,775
_2421
Pf6N2E2_2422 - Molybdopterin biosynthesis protein MoeA, at 2,872,835 to 2,874,061
_2422
Pf6N2E2_2423 - Molybdenum cofactor biosynthesis protein MoaB, at 2,874,045 to 2,874,584
_2423
Pf6N2E2_2424 - Molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 2,874,663 to 2,875,265
_2424
Position (kb)
2872
2873
2874
2875 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2871.862 kb on + strand, within Pf6N2E2_2419 at 2871.948 kb on + strand, within Pf6N2E2_2419 at 2871.948 kb on + strand, within Pf6N2E2_2419 at 2871.949 kb on - strand, within Pf6N2E2_2419 at 2871.949 kb on - strand, within Pf6N2E2_2419 at 2871.949 kb on - strand, within Pf6N2E2_2419 at 2871.949 kb on - strand, within Pf6N2E2_2419 at 2871.949 kb on - strand, within Pf6N2E2_2419 at 2872.195 kb on - strand, within Pf6N2E2_2420 at 2872.195 kb on - strand, within Pf6N2E2_2420 at 2872.281 kb on + strand, within Pf6N2E2_2420 at 2872.282 kb on - strand, within Pf6N2E2_2420 at 2872.359 kb on - strand at 2872.386 kb on + strand at 2872.387 kb on - strand at 2872.387 kb on - strand at 2872.400 kb on + strand at 2872.400 kb on + strand at 2872.401 kb on - strand at 2872.401 kb on - strand at 2872.442 kb on + strand at 2872.442 kb on + strand at 2872.442 kb on + strand at 2872.472 kb on + strand at 2872.473 kb on - strand at 2872.482 kb on + strand at 2872.482 kb on + strand at 2872.482 kb on + strand at 2872.482 kb on + strand at 2872.482 kb on + strand at 2872.483 kb on - strand at 2872.483 kb on - strand at 2872.532 kb on + strand at 2872.533 kb on - strand at 2872.559 kb on + strand at 2872.560 kb on - strand at 2872.620 kb on + strand, within Pf6N2E2_2421 at 2872.620 kb on + strand, within Pf6N2E2_2421 at 2872.620 kb on + strand, within Pf6N2E2_2421 at 2872.620 kb on + strand, within Pf6N2E2_2421 at 2872.621 kb on - strand, within Pf6N2E2_2421 at 2872.621 kb on - strand, within Pf6N2E2_2421 at 2872.621 kb on - strand, within Pf6N2E2_2421 at 2872.621 kb on - strand, within Pf6N2E2_2421 at 2872.621 kb on - strand, within Pf6N2E2_2421 at 2872.657 kb on + strand, within Pf6N2E2_2421 at 2872.657 kb on + strand, within Pf6N2E2_2421 at 2872.657 kb on + strand at 2872.658 kb on - strand, within Pf6N2E2_2421 at 2872.683 kb on + strand, within Pf6N2E2_2421 at 2872.683 kb on + strand, within Pf6N2E2_2421 at 2872.684 kb on - strand, within Pf6N2E2_2421 at 2872.684 kb on - strand, within Pf6N2E2_2421 at 2873.003 kb on + strand, within Pf6N2E2_2422 at 2873.004 kb on + strand, within Pf6N2E2_2422 at 2873.004 kb on + strand, within Pf6N2E2_2422 at 2873.004 kb on + strand, within Pf6N2E2_2422 at 2873.005 kb on - strand, within Pf6N2E2_2422 at 2873.005 kb on - strand, within Pf6N2E2_2422 at 2873.078 kb on - strand, within Pf6N2E2_2422 at 2873.093 kb on + strand, within Pf6N2E2_2422 at 2873.093 kb on + strand, within Pf6N2E2_2422 at 2873.093 kb on + strand, within Pf6N2E2_2422 at 2873.100 kb on + strand, within Pf6N2E2_2422 at 2873.159 kb on + strand, within Pf6N2E2_2422 at 2873.159 kb on + strand, within Pf6N2E2_2422 at 2873.178 kb on + strand, within Pf6N2E2_2422 at 2873.351 kb on + strand, within Pf6N2E2_2422 at 2873.369 kb on - strand, within Pf6N2E2_2422 at 2873.435 kb on - strand, within Pf6N2E2_2422 at 2873.435 kb on - strand, within Pf6N2E2_2422 at 2873.436 kb on + strand, within Pf6N2E2_2422 at 2873.436 kb on + strand, within Pf6N2E2_2422 at 2873.436 kb on + strand, within Pf6N2E2_2422 at 2873.436 kb on + strand, within Pf6N2E2_2422 at 2873.436 kb on + strand, within Pf6N2E2_2422 at 2873.437 kb on - strand, within Pf6N2E2_2422 at 2873.437 kb on - strand, within Pf6N2E2_2422 at 2873.437 kb on - strand, within Pf6N2E2_2422 at 2873.437 kb on - strand, within Pf6N2E2_2422 at 2873.437 kb on - strand, within Pf6N2E2_2422 at 2873.437 kb on - strand, within Pf6N2E2_2422 at 2873.857 kb on - strand, within Pf6N2E2_2422 at 2873.857 kb on - strand, within Pf6N2E2_2422 at 2873.867 kb on - strand, within Pf6N2E2_2422 at 2874.186 kb on + strand, within Pf6N2E2_2423 at 2874.186 kb on + strand, within Pf6N2E2_2423 at 2874.186 kb on + strand, within Pf6N2E2_2423 at 2874.187 kb on - strand, within Pf6N2E2_2423 at 2874.187 kb on - strand, within Pf6N2E2_2423 at 2874.418 kb on + strand, within Pf6N2E2_2423 at 2874.418 kb on + strand, within Pf6N2E2_2423 at 2874.418 kb on + strand, within Pf6N2E2_2423 at 2874.419 kb on - strand, within Pf6N2E2_2423 at 2874.501 kb on - strand, within Pf6N2E2_2423 at 2874.501 kb on - strand, within Pf6N2E2_2423 at 2874.515 kb on + strand, within Pf6N2E2_2423 at 2874.521 kb on + strand, within Pf6N2E2_2423 at 2874.521 kb on + strand, within Pf6N2E2_2423 at 2874.521 kb on + strand, within Pf6N2E2_2423 at 2874.522 kb on - strand, within Pf6N2E2_2423 at 2874.564 kb on + strand at 2874.564 kb on + strand at 2874.564 kb on + strand at 2874.564 kb on + strand at 2874.565 kb on - strand at 2874.565 kb on - strand at 2874.565 kb on - strand at 2874.620 kb on + strand at 2874.620 kb on + strand at 2874.620 kb on + strand at 2874.620 kb on + strand at 2874.620 kb on + strand at 2874.620 kb on + strand at 2874.620 kb on + strand at 2874.620 kb on + strand at 2874.621 kb on - strand at 2874.621 kb on - strand at 2874.621 kb on - strand at 2874.621 kb on - strand at 2874.621 kb on - strand at 2874.621 kb on - strand at 2874.621 kb on - strand at 2874.621 kb on - strand at 2874.622 kb on + strand at 2874.622 kb on + strand at 2874.623 kb on - strand at 2874.840 kb on - strand, within Pf6N2E2_2424 at 2874.861 kb on + strand, within Pf6N2E2_2424 at 2874.861 kb on + strand, within Pf6N2E2_2424 at 2874.861 kb on + strand, within Pf6N2E2_2424 at 2874.862 kb on - strand, within Pf6N2E2_2424 at 2874.951 kb on + strand, within Pf6N2E2_2424 at 2874.952 kb on - strand, within Pf6N2E2_2424
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 2,871,862 + Pf6N2E2_2419 0.72 -0.8 2,871,948 + Pf6N2E2_2419 0.81 +0.7 2,871,948 + Pf6N2E2_2419 0.81 -0.3 2,871,949 - Pf6N2E2_2419 0.81 +1.2 2,871,949 - Pf6N2E2_2419 0.81 +0.4 2,871,949 - Pf6N2E2_2419 0.81 -0.5 2,871,949 - Pf6N2E2_2419 0.81 -0.5 2,871,949 - Pf6N2E2_2419 0.81 -0.4 2,872,195 - Pf6N2E2_2420 0.27 +1.1 2,872,195 - Pf6N2E2_2420 0.27 +1.5 2,872,281 + Pf6N2E2_2420 0.78 -0.0 2,872,282 - Pf6N2E2_2420 0.79 -0.5 2,872,359 - +0.2 2,872,386 + -0.7 2,872,387 - +0.4 2,872,387 - +0.7 2,872,400 + -0.3 2,872,400 + +0.3 2,872,401 - +0.9 2,872,401 - -1.2 2,872,442 + -0.9 2,872,442 + -0.4 2,872,442 + -0.4 2,872,472 + -1.0 2,872,473 - +0.3 2,872,482 + -1.1 2,872,482 + -0.0 2,872,482 + +0.2 2,872,482 + +0.2 2,872,482 + -1.0 2,872,483 - -0.6 2,872,483 - -0.8 2,872,532 + +0.1 2,872,533 - -0.7 2,872,559 + +0.3 2,872,560 - -0.6 2,872,620 + Pf6N2E2_2421 0.20 -0.3 2,872,620 + Pf6N2E2_2421 0.20 +0.6 2,872,620 + Pf6N2E2_2421 0.20 -0.2 2,872,620 + Pf6N2E2_2421 0.20 +0.0 2,872,621 - Pf6N2E2_2421 0.21 +0.2 2,872,621 - Pf6N2E2_2421 0.21 -0.1 2,872,621 - Pf6N2E2_2421 0.21 -0.1 2,872,621 - Pf6N2E2_2421 0.21 +0.0 2,872,621 - Pf6N2E2_2421 0.21 +0.6 2,872,657 + Pf6N2E2_2421 0.39 -1.6 2,872,657 + Pf6N2E2_2421 0.39 -0.9 2,872,657 + +3.1 2,872,658 - Pf6N2E2_2421 0.39 -0.4 2,872,683 + Pf6N2E2_2421 0.52 +0.2 2,872,683 + Pf6N2E2_2421 0.52 -0.7 2,872,684 - Pf6N2E2_2421 0.53 -0.2 2,872,684 - Pf6N2E2_2421 0.53 -0.1 2,873,003 + Pf6N2E2_2422 0.14 +0.5 2,873,004 + Pf6N2E2_2422 0.14 -0.4 2,873,004 + Pf6N2E2_2422 0.14 -1.4 2,873,004 + Pf6N2E2_2422 0.14 -1.8 2,873,005 - Pf6N2E2_2422 0.14 -1.1 2,873,005 - Pf6N2E2_2422 0.14 -1.8 2,873,078 - Pf6N2E2_2422 0.20 -1.8 2,873,093 + Pf6N2E2_2422 0.21 +0.6 2,873,093 + Pf6N2E2_2422 0.21 -1.0 2,873,093 + Pf6N2E2_2422 0.21 -1.5 2,873,100 + Pf6N2E2_2422 0.22 -0.3 2,873,159 + Pf6N2E2_2422 0.26 -1.0 2,873,159 + Pf6N2E2_2422 0.26 -0.6 2,873,178 + Pf6N2E2_2422 0.28 -0.6 2,873,351 + Pf6N2E2_2422 0.42 -1.3 2,873,369 - Pf6N2E2_2422 0.44 -3.5 2,873,435 - Pf6N2E2_2422 0.49 +0.2 2,873,435 - Pf6N2E2_2422 0.49 -3.1 2,873,436 + Pf6N2E2_2422 0.49 +0.6 2,873,436 + Pf6N2E2_2422 0.49 -0.8 2,873,436 + Pf6N2E2_2422 0.49 -1.1 2,873,436 + Pf6N2E2_2422 0.49 -1.0 2,873,436 + Pf6N2E2_2422 0.49 -1.7 2,873,437 - Pf6N2E2_2422 0.49 -1.4 2,873,437 - Pf6N2E2_2422 0.49 +0.2 2,873,437 - Pf6N2E2_2422 0.49 -1.2 2,873,437 - Pf6N2E2_2422 0.49 -0.6 2,873,437 - Pf6N2E2_2422 0.49 +0.1 2,873,437 - Pf6N2E2_2422 0.49 -1.7 2,873,857 - Pf6N2E2_2422 0.83 -1.3 2,873,857 - Pf6N2E2_2422 0.83 -1.8 2,873,867 - Pf6N2E2_2422 0.84 -1.1 2,874,186 + Pf6N2E2_2423 0.26 -1.0 2,874,186 + Pf6N2E2_2423 0.26 -0.9 2,874,186 + Pf6N2E2_2423 0.26 +0.0 2,874,187 - Pf6N2E2_2423 0.26 -1.5 2,874,187 - Pf6N2E2_2423 0.26 -0.5 2,874,418 + Pf6N2E2_2423 0.69 -1.7 2,874,418 + Pf6N2E2_2423 0.69 -0.2 2,874,418 + Pf6N2E2_2423 0.69 -0.2 2,874,419 - Pf6N2E2_2423 0.69 +0.5 2,874,501 - Pf6N2E2_2423 0.84 -0.7 2,874,501 - Pf6N2E2_2423 0.84 -1.3 2,874,515 + Pf6N2E2_2423 0.87 +0.6 2,874,521 + Pf6N2E2_2423 0.88 -1.4 2,874,521 + Pf6N2E2_2423 0.88 -1.1 2,874,521 + Pf6N2E2_2423 0.88 +0.1 2,874,522 - Pf6N2E2_2423 0.88 -1.4 2,874,564 + -0.9 2,874,564 + -0.7 2,874,564 + -1.0 2,874,564 + +0.0 2,874,565 - -0.9 2,874,565 - -0.9 2,874,565 - -0.8 2,874,620 + +0.2 2,874,620 + -0.4 2,874,620 + -1.3 2,874,620 + -0.4 2,874,620 + -0.2 2,874,620 + -1.3 2,874,620 + -0.5 2,874,620 + -0.6 2,874,621 - +0.8 2,874,621 - -1.8 2,874,621 - +0.0 2,874,621 - -0.2 2,874,621 - -0.4 2,874,621 - +0.0 2,874,621 - -1.1 2,874,621 - -0.4 2,874,622 + -3.1 2,874,622 + -0.3 2,874,623 - +1.0 2,874,840 - Pf6N2E2_2424 0.29 -1.4 2,874,861 + Pf6N2E2_2424 0.33 -0.3 2,874,861 + Pf6N2E2_2424 0.33 -0.6 2,874,861 + Pf6N2E2_2424 0.33 -0.5 2,874,862 - Pf6N2E2_2424 0.33 -0.0 2,874,951 + Pf6N2E2_2424 0.48 -0.2 2,874,952 - Pf6N2E2_2424 0.48 +0.7
Or see this region's nucleotide sequence